Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACOFKY_RS12025 Genome accession   NZ_OZ241355
Coordinates   2588216..2589253 (+) Length   345 a.a.
NCBI ID   WP_336952893.1    Uniprot ID   -
Organism   MAG: Acinetobacter sp. isolate 91b77bb6-a760-4576-a270-dd86adb6cd71     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2583216..2594253
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACOFKY_RS12005 - 2583815..2584606 (-) 792 WP_419327571.1 hypothetical protein -
  ACOFKY_RS12010 - 2584897..2585700 (-) 804 WP_419819566.1 thioesterase family protein -
  ACOFKY_RS12015 - 2585888..2586721 (-) 834 WP_419819567.1 undecaprenyl-diphosphate phosphatase -
  ACOFKY_RS12020 - 2587124..2587816 (-) 693 WP_336952892.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACOFKY_RS12025 pilT 2588216..2589253 (+) 1038 WP_336952893.1 type IV pilus twitching motility protein PilT Machinery gene
  ACOFKY_RS12030 pilU 2589279..2590397 (+) 1119 WP_336952895.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACOFKY_RS12035 fur 2590475..2590912 (-) 438 WP_336952896.1 ferric iron uptake transcriptional regulator -
  ACOFKY_RS12040 - 2591025..2591423 (+) 399 WP_004722739.1 outer membrane protein assembly factor BamE -
  ACOFKY_RS12045 - 2591508..2591837 (-) 330 WP_336952898.1 RnfH family protein -
  ACOFKY_RS12050 - 2591846..2592913 (-) 1068 WP_336952899.1 hypothetical protein -
  ACOFKY_RS12055 - 2593132..2593587 (-) 456 WP_004819397.1 bacteriohemerythrin -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38330.07 Da        Isoelectric Point: 6.5057

>NTDB_id=1170773 ACOFKY_RS12025 WP_336952893.1 2588216..2589253(+) (pilT) [MAG: Acinetobacter sp. isolate 91b77bb6-a760-4576-a270-dd86adb6cd71]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKEVHRLVYDIMNDKQRRDFEEQLETDFSFEVPSIAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGQIFKDLCELPRGLILVTGPTGSGKSTTLASMIDFINDSRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANYGMTTLDQSL
KTLVAKGIISPQVARNVAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1170773 ACOFKY_RS12025 WP_336952893.1 2588216..2589253(+) (pilT) [MAG: Acinetobacter sp. isolate 91b77bb6-a760-4576-a270-dd86adb6cd71]
ATGGATATTACAGAATTATTGGCGTTTTCCGTCAAAAATGGTGCATCTGACTTACACCTTTCAGCCGGTATGCCACCGAT
GATTCGTGTCGATGGGGAAGTCCGTCGTATCAATTTACCAGCATTGGAACATAAAGAAGTTCACAGACTGGTTTATGACA
TCATGAATGATAAACAACGTCGTGATTTTGAAGAACAATTAGAAACAGACTTCTCATTTGAAGTTCCAAGTATTGCCCGT
TTTCGTGTAAACGCATTCAATCAAAATCGCGGTGCTGGTGCTGTATTCCGTACCATTCCGTCCAAAGTTCTGACCATGGA
AGATTTAGGTTTAGGACAAATCTTCAAAGATCTTTGTGAATTACCACGTGGTTTGATTCTTGTAACAGGCCCAACAGGTT
CGGGGAAGTCAACCACATTGGCCTCTATGATTGACTTTATCAATGACAGTCGCTATGACCATATTTTAACAGTCGAAGAC
CCAATCGAATTTGTACACCAATCTAAGAAGTGCCTCATCAACCAGCGTGAAGTGCATCGTGATACACATGGCTTTAATGA
AGCACTGCGTTCTGCGTTACGTGAAGACCCTGACATTATCTTGGTCGGTGAGATGCGAGACCTTGAAACCATTCGCCTTG
CATTAACTGCTGCAGAAACAGGTCACTTGGTTTTCGGCACGCTACATACCACATCTGCGGCAAAAACCATTGACCGTGTC
ATTGACGTATTCCCTGCTGAAGAAAAAGATATGGTACGCGCCATGTTGTCAGAATCATTACAAGCCGTCATTTCGCAAAC
ACTGCTTAAAAAGAATGGTGGAGGACGGGTTGCCGCACATGAAATTATGATTGGTATTCCTGCAATTCGTAACTTAATCC
GTGAAAACAAAGTTGCACAGATGTATTCAGCAATTCAAACGGGTGCAAACTATGGTATGACCACCTTAGATCAAAGTTTA
AAAACACTGGTTGCCAAAGGTATTATTAGTCCTCAAGTTGCAAGAAATGTAGCAAAACAACCAGAATCATTTTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

93.623

100

0.936
  pilT Acinetobacter baumannii strain A118

93.623

100

0.936
  pilT Acinetobacter nosocomialis M2

93.333

100

0.933
  pilT Acinetobacter baylyi ADP1

86.919

99.71

0.867
  pilT Pseudomonas aeruginosa PAK

83.14

99.71

0.829
  pilT Pseudomonas stutzeri DSM 10701

82.558

99.71

0.823
  pilT Legionella pneumophila strain Lp02

75.74

97.971

0.742
  pilT Legionella pneumophila strain ERS1305867

75.74

97.971

0.742
  pilT Neisseria meningitidis 8013

66.957

100

0.67
  pilT Neisseria gonorrhoeae MS11

66.377

100

0.664
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.976

96.232

0.664
  pilT Vibrio cholerae strain A1552

68.976

96.232

0.664
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

100

0.516
  pilU Vibrio cholerae strain A1552

41.298

98.261

0.406
  pilU Acinetobacter baylyi ADP1

39.884

100

0.4
  pilU Pseudomonas stutzeri DSM 10701

41.088

95.942

0.394

Multiple sequence alignment