Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACOFKY_RS12030 Genome accession   NZ_OZ241355
Coordinates   2589279..2590397 (+) Length   372 a.a.
NCBI ID   WP_336952895.1    Uniprot ID   -
Organism   MAG: Acinetobacter sp. isolate 91b77bb6-a760-4576-a270-dd86adb6cd71     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2584279..2595397
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACOFKY_RS12010 - 2584897..2585700 (-) 804 WP_419819566.1 thioesterase family protein -
  ACOFKY_RS12015 - 2585888..2586721 (-) 834 WP_419819567.1 undecaprenyl-diphosphate phosphatase -
  ACOFKY_RS12020 - 2587124..2587816 (-) 693 WP_336952892.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACOFKY_RS12025 pilT 2588216..2589253 (+) 1038 WP_336952893.1 type IV pilus twitching motility protein PilT Machinery gene
  ACOFKY_RS12030 pilU 2589279..2590397 (+) 1119 WP_336952895.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACOFKY_RS12035 fur 2590475..2590912 (-) 438 WP_336952896.1 ferric iron uptake transcriptional regulator -
  ACOFKY_RS12040 - 2591025..2591423 (+) 399 WP_004722739.1 outer membrane protein assembly factor BamE -
  ACOFKY_RS12045 - 2591508..2591837 (-) 330 WP_336952898.1 RnfH family protein -
  ACOFKY_RS12050 - 2591846..2592913 (-) 1068 WP_336952899.1 hypothetical protein -
  ACOFKY_RS12055 - 2593132..2593587 (-) 456 WP_004819397.1 bacteriohemerythrin -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41853.16 Da        Isoelectric Point: 6.2461

>NTDB_id=1170774 ACOFKY_RS12030 WP_336952895.1 2589279..2590397(+) (pilU) [MAG: Acinetobacter sp. isolate 91b77bb6-a760-4576-a270-dd86adb6cd71]
MDFNDLLNMMIEQKASDLFITADVEPSMKINGQIVPVVKTKLNGEIIMQLLNSIMSEKQRKEFAETRECNFAITNRDKTA
RFRVSAFQQRDMPGMVLRRIETQIPTMDELKLPPILKDLAMTKRGIIIFVGATGTGKSTSLASIIGYRNQNSKGHIITIE
DPIEFVHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVFATLHANNANQAIDR
IIHFFESDRHSQLFMDLSLNLKAMVAQQLIPTPDGNSRRAAIEILINSPLVSDLIRKGDVHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLQIKLDEEGGAQLLNANSKLTFDGQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=1170774 ACOFKY_RS12030 WP_336952895.1 2589279..2590397(+) (pilU) [MAG: Acinetobacter sp. isolate 91b77bb6-a760-4576-a270-dd86adb6cd71]
ATGGATTTTAATGACTTGCTCAATATGATGATTGAGCAAAAAGCATCAGACTTATTTATTACTGCCGATGTTGAACCATC
AATGAAAATTAATGGTCAAATTGTTCCTGTAGTAAAAACCAAGTTAAACGGCGAAATTATTATGCAACTGCTTAACTCAA
TTATGAGTGAAAAGCAGCGTAAAGAATTTGCTGAGACGCGTGAATGTAACTTTGCCATTACCAATCGCGATAAAACTGCT
CGTTTCCGTGTGAGTGCCTTTCAGCAGCGTGATATGCCAGGTATGGTTTTGCGTCGTATTGAAACTCAAATTCCAACTAT
GGATGAGCTTAAATTACCACCTATCCTCAAAGATCTGGCAATGACCAAACGTGGAATTATCATCTTTGTCGGAGCAACAG
GGACAGGTAAATCAACTTCGCTTGCGTCTATCATTGGTTATCGTAATCAAAACTCTAAAGGTCATATCATCACCATTGAA
GACCCGATTGAATTTGTGCATGAACATGCAGGATGCATTATTACGCAACGTGAAGTGGGAATCGATACAGATTCTTTTGA
AATTGCGCTAAAAAATACTTTGCGCCAAGCGCCAGATGTGATCTTGATTGGTGAAATTCGTTCTCGTGAAACCATGGACT
ATGCCATAGCCTTTGCTGAAACGGGACACTTAGTATTTGCCACACTGCATGCCAACAACGCAAACCAAGCAATTGATCGG
ATTATTCACTTCTTTGAGTCTGATCGTCATAGCCAGTTGTTTATGGATCTATCGCTCAATTTAAAAGCGATGGTAGCACA
GCAATTAATCCCAACACCTGATGGTAATTCACGTCGTGCTGCAATTGAAATTCTAATCAACTCACCATTGGTTTCAGACT
TAATCCGTAAAGGTGATGTGCATGAGATTAAGGATTTGATGAAACGCTCTCGTGAATTGGGTATGCAAACTTTTGACCAA
GCTTTATTTGACCTTTATAAAGCAGGACAAATTACCTATAAAGATGCACTTAAACATGCTGACTCTCCAAATGATTTACG
TCTACAAATCAAATTGGATGAAGAAGGTGGTGCACAACTACTTAATGCAAATAGTAAACTCACCTTTGATGGACAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-250)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

76.455

100

0.777
  pilU Pseudomonas stutzeri DSM 10701

68.464

99.731

0.683
  pilU Vibrio cholerae strain A1552

54.595

99.462

0.543
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.353

93.011

0.403
  pilT Legionella pneumophila strain Lp02

40.828

90.86

0.371
  pilT Legionella pneumophila strain ERS1305867

40.828

90.86

0.371
  pilT Acinetobacter baumannii D1279779

39.65

92.204

0.366
  pilT Acinetobacter baylyi ADP1

39.65

92.204

0.366
  pilT Acinetobacter nosocomialis M2

39.65

92.204

0.366
  pilT Acinetobacter baumannii strain A118

39.65

92.204

0.366
  pilT Pseudomonas aeruginosa PAK

39.306

93.011

0.366
  pilT Neisseria meningitidis 8013

39.412

91.398

0.36
  pilT Neisseria gonorrhoeae MS11

39.412

91.398

0.36

Multiple sequence alignment