Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACOCLE_RS19025 Genome accession   NZ_OZ239981
Coordinates   4224081..4225115 (-) Length   344 a.a.
NCBI ID   WP_419533792.1    Uniprot ID   -
Organism   MAG: Endozoicomonas sp. isolate 83fe6ec3-ea9b-4b46-8e1a-20fbcf80582c     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4219081..4230115
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACOCLE_RS19000 gshB 4219634..4220590 (+) 957 WP_419533787.1 glutathione synthase -
  ACOCLE_RS19005 - 4220762..4221652 (+) 891 WP_419533788.1 energy transducer TonB -
  ACOCLE_RS19010 - 4221723..4222295 (+) 573 WP_419533789.1 YqgE/AlgH family protein -
  ACOCLE_RS19015 ruvX 4222307..4222756 (+) 450 WP_419533790.1 Holliday junction resolvase RuvX -
  ACOCLE_RS19020 pilU 4222801..4223925 (-) 1125 WP_419533791.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACOCLE_RS19025 pilT 4224081..4225115 (-) 1035 WP_419533792.1 type IV pilus twitching motility protein PilT Machinery gene
  ACOCLE_RS19030 - 4225184..4225882 (+) 699 WP_419533793.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACOCLE_RS19035 proC 4225889..4226710 (+) 822 WP_419533794.1 pyrroline-5-carboxylate reductase -
  ACOCLE_RS19040 - 4226890..4227384 (+) 495 WP_419533795.1 YggT family protein -
  ACOCLE_RS19045 - 4227528..4230008 (+) 2481 WP_419533796.1 glycoside hydrolase domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38240.15 Da        Isoelectric Point: 7.6294

>NTDB_id=1170743 ACOCLE_RS19025 WP_419533792.1 4224081..4225115(-) (pilT) [MAG: Endozoicomonas sp. isolate 83fe6ec3-ea9b-4b46-8e1a-20fbcf80582c]
MDITELLAFSFKQGASDLHLSAGLPPIIRMDGDVRRINLPPMDQKKIHGLIYDIMNDKQRKDFEEHLETDFSFEVPGVAR
FRVNAFNHNRGTGAVFRTIPSKVLTMDDLSMGQVFKTIADKPRGLVLVTGPTGSGKSTTLAAMLDYINDTKYHHILTIED
PIEFIHTPKKCLINQREVHRDTHSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRI
VDVFPAEEKSMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMLGTAGIRNLIREDKVAQMYSAIQTGGGLGMQTLDQCL
MNLMKKGLITRDVAREKAKDRDAF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1170743 ACOCLE_RS19025 WP_419533792.1 4224081..4225115(-) (pilT) [MAG: Endozoicomonas sp. isolate 83fe6ec3-ea9b-4b46-8e1a-20fbcf80582c]
ATGGATATTACTGAGCTGTTGGCATTCAGCTTTAAACAGGGGGCTTCGGATCTGCATCTGTCAGCAGGGCTTCCGCCAAT
TATTCGCATGGATGGCGATGTGCGTCGTATTAATCTGCCGCCCATGGATCAGAAGAAAATTCATGGGTTGATCTACGATA
TCATGAATGACAAGCAGCGAAAGGACTTCGAGGAACATCTGGAAACAGACTTCTCCTTTGAGGTACCTGGTGTTGCCCGT
TTCCGTGTGAATGCCTTCAATCATAACCGGGGTACAGGTGCTGTATTTCGTACCATTCCCAGTAAAGTGCTGACCATGGA
TGATTTGAGCATGGGGCAGGTGTTTAAAACCATTGCCGACAAGCCCAGAGGGCTCGTACTGGTTACCGGCCCTACTGGCT
CTGGTAAGAGTACAACGCTGGCTGCCATGCTGGATTACATCAATGACACCAAATACCACCATATCCTGACCATTGAAGAC
CCTATTGAATTTATCCACACTCCGAAGAAATGTCTGATAAACCAGCGGGAAGTACACCGGGATACCCATAGCTTCAGTAA
CGCTCTGAGGTCGGCGCTGCGTGAAGACCCGGATATTATTCTCGTTGGTGAGATGCGAGACCTGGAAACTATCCGTCTGG
CGATGACTGCGGCTGAAACCGGTCACCTGGTGTTTGGTACTCTGCACACCACTTCGGCAGCCAAAACCATTGACCGTATT
GTCGATGTCTTCCCAGCGGAAGAGAAGTCGATGGTTCGTGCCATGCTTTCTGAATCGCTTCAGGCTGTGATCTCGCAAAC
ACTTTTGAAGAAAAACGGTGGAGGCCGTGTTGCGGCCCATGAGATCATGCTCGGTACTGCAGGTATTCGAAATCTGATTC
GGGAAGATAAGGTTGCCCAGATGTACTCGGCCATTCAGACTGGTGGTGGGTTAGGCATGCAAACATTGGATCAGTGTCTG
ATGAACCTGATGAAAAAGGGACTGATCACCCGTGATGTTGCTCGTGAAAAGGCCAAGGATCGAGACGCATTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

85.756

100

0.858
  pilT Pseudomonas aeruginosa PAK

84.884

100

0.849
  pilT Acinetobacter nosocomialis M2

79.942

100

0.799
  pilT Acinetobacter baumannii D1279779

79.942

100

0.799
  pilT Acinetobacter baumannii strain A118

79.942

100

0.799
  pilT Acinetobacter baylyi ADP1

77.907

100

0.779
  pilT Legionella pneumophila strain Lp02

71.221

100

0.712
  pilT Legionella pneumophila strain ERS1305867

71.221

100

0.712
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.087

100

0.663
  pilT Vibrio cholerae strain A1552

66.087

100

0.663
  pilT Neisseria meningitidis 8013

65.217

100

0.654
  pilT Neisseria gonorrhoeae MS11

64.638

100

0.648
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.333

100

0.535
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.384

0.404
  pilU Acinetobacter baylyi ADP1

40.923

94.477

0.387
  pilU Vibrio cholerae strain A1552

40.308

94.477

0.381

Multiple sequence alignment