Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACOCLE_RS19020 Genome accession   NZ_OZ239981
Coordinates   4222801..4223925 (-) Length   374 a.a.
NCBI ID   WP_419533791.1    Uniprot ID   -
Organism   MAG: Endozoicomonas sp. isolate 83fe6ec3-ea9b-4b46-8e1a-20fbcf80582c     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4217801..4228925
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACOCLE_RS18995 asd 4218712..4219614 (+) 903 WP_419533786.1 archaetidylserine decarboxylase -
  ACOCLE_RS19000 gshB 4219634..4220590 (+) 957 WP_419533787.1 glutathione synthase -
  ACOCLE_RS19005 - 4220762..4221652 (+) 891 WP_419533788.1 energy transducer TonB -
  ACOCLE_RS19010 - 4221723..4222295 (+) 573 WP_419533789.1 YqgE/AlgH family protein -
  ACOCLE_RS19015 ruvX 4222307..4222756 (+) 450 WP_419533790.1 Holliday junction resolvase RuvX -
  ACOCLE_RS19020 pilU 4222801..4223925 (-) 1125 WP_419533791.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACOCLE_RS19025 pilT 4224081..4225115 (-) 1035 WP_419533792.1 type IV pilus twitching motility protein PilT Machinery gene
  ACOCLE_RS19030 - 4225184..4225882 (+) 699 WP_419533793.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACOCLE_RS19035 proC 4225889..4226710 (+) 822 WP_419533794.1 pyrroline-5-carboxylate reductase -
  ACOCLE_RS19040 - 4226890..4227384 (+) 495 WP_419533795.1 YggT family protein -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 41972.56 Da        Isoelectric Point: 6.7793

>NTDB_id=1170742 ACOCLE_RS19020 WP_419533791.1 4222801..4223925(-) (pilU) [MAG: Endozoicomonas sp. isolate 83fe6ec3-ea9b-4b46-8e1a-20fbcf80582c]
MDIEKLLKLVSVKKASDLFITAGFPPSVKLDGRLMPVTKTPLTATEAKDMVLGVMDEKQRLEFELKQECNFAIAKVGIGR
FRVSAFQQRNQSGMVLRRIEEKIPSFEDLKLPHILKDLTMTKRGLVILVGATGTGKSTTLAAMLGYRNENETGHIISIED
PIEFIHQHKKCIVTQREVGIDTKSYEVALKNTLRQAPDVIMIGEIRSQDTMQYALNFAETGHLCLATLHANNANQALDRI
LHFFPPEHHHQVWMDLSLNLKGIVAQQLIPSLDGTGRRPAIEVLLNTPLAQDMIRKGDVHALKELMKKSRELGMQTFDQA
LFDLYNSGEISYEEAIRHADSANDLRLMIKLGSEVDGDYLTERSGKLTLQMDEA

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=1170742 ACOCLE_RS19020 WP_419533791.1 4222801..4223925(-) (pilU) [MAG: Endozoicomonas sp. isolate 83fe6ec3-ea9b-4b46-8e1a-20fbcf80582c]
ATGGATATTGAAAAATTATTAAAACTGGTCTCCGTTAAAAAAGCATCGGATCTGTTTATCACCGCAGGTTTTCCTCCCAG
TGTAAAGCTTGATGGACGTCTGATGCCTGTCACAAAGACACCACTGACTGCCACAGAGGCAAAAGATATGGTGCTTGGTG
TCATGGATGAAAAGCAACGTCTTGAGTTTGAGTTGAAGCAGGAGTGTAACTTTGCCATAGCCAAGGTGGGCATTGGCCGC
TTTCGAGTCAGTGCTTTCCAGCAAAGAAACCAGTCAGGCATGGTCTTGAGGCGAATTGAGGAGAAAATTCCCAGCTTTGA
AGATTTGAAGCTACCTCATATCCTTAAAGATCTTACCATGACCAAGCGCGGGCTTGTTATCCTGGTGGGCGCTACTGGTA
CGGGTAAGTCAACAACACTGGCCGCGATGCTTGGCTATCGAAATGAAAATGAAACGGGACATATCATCAGCATTGAAGAC
CCGATTGAGTTTATCCATCAGCATAAGAAGTGCATCGTTACGCAACGCGAAGTGGGAATCGATACCAAGTCCTATGAAGT
GGCGCTGAAGAACACGCTTCGTCAGGCTCCGGATGTCATTATGATCGGTGAGATTCGAAGTCAGGATACCATGCAGTACG
CCCTGAACTTTGCAGAAACCGGTCATCTTTGTCTGGCAACACTTCATGCCAACAATGCCAACCAGGCATTGGATCGCATC
CTGCATTTCTTTCCCCCGGAGCACCATCATCAGGTCTGGATGGATCTGTCCCTCAACTTGAAAGGGATTGTTGCCCAGCA
GCTGATTCCTTCACTGGATGGAACCGGACGCCGTCCAGCTATAGAGGTGCTTCTGAATACACCACTGGCTCAGGATATGA
TTCGCAAAGGTGATGTTCATGCATTGAAAGAGCTGATGAAAAAGTCCCGGGAGCTGGGTATGCAGACCTTCGATCAGGCG
TTGTTTGACCTCTACAACAGTGGAGAGATCAGCTATGAAGAAGCAATCCGGCATGCGGATTCTGCAAACGACCTGCGCCT
GATGATTAAGCTTGGCTCTGAAGTGGATGGGGATTATCTGACGGAGCGATCAGGCAAACTGACTTTGCAAATGGATGAGG
CTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

70.509

99.733

0.703
  pilU Acinetobacter baylyi ADP1

62.465

95.455

0.596
  pilU Vibrio cholerae strain A1552

57.714

93.583

0.54
  pilT Acinetobacter baumannii strain A118

42.012

90.374

0.38
  pilT Acinetobacter nosocomialis M2

42.012

90.374

0.38
  pilT Acinetobacter baumannii D1279779

42.012

90.374

0.38
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.298

90.642

0.374
  pilT Legionella pneumophila strain Lp02

41.493

89.572

0.372
  pilT Legionella pneumophila strain ERS1305867

41.493

89.572

0.372
  pilT Pseudomonas aeruginosa PAK

41.194

89.572

0.369

Multiple sequence alignment