Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACNALX_RS02160 Genome accession   NZ_OZ223210
Coordinates   426753..427790 (+) Length   345 a.a.
NCBI ID   WP_217394146.1    Uniprot ID   -
Organism   MAG: Photobacterium ganghwense isolate daa05ea6-4f3f-4f7c-82d4-3ed073323da5     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 421753..432790
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNALX_RS02130 - 422827..423420 (-) 594 WP_415720010.1 XTP/dITP diphosphatase -
  ACNALX_RS02135 - 423513..423950 (-) 438 WP_047887449.1 DUF4426 domain-containing protein -
  ACNALX_RS02140 yggU 424084..424377 (-) 294 WP_273857529.1 DUF167 family protein YggU -
  ACNALX_RS02145 - 424374..424928 (-) 555 WP_047887447.1 YggT family protein -
  ACNALX_RS02150 proC 424942..425760 (-) 819 WP_273857528.1 pyrroline-5-carboxylate reductase -
  ACNALX_RS02155 - 426013..426729 (-) 717 WP_273857527.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACNALX_RS02160 pilT 426753..427790 (+) 1038 WP_217394146.1 type IV pilus twitching motility protein PilT Machinery gene
  ACNALX_RS02165 pilU 427815..428921 (+) 1107 WP_047887443.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACNALX_RS02170 ruvX 429426..429854 (-) 429 WP_217394142.1 Holliday junction resolvase RuvX -
  ACNALX_RS02175 - 429851..430417 (-) 567 WP_047887441.1 YqgE/AlgH family protein -
  ACNALX_RS02180 gshB 430548..431498 (-) 951 WP_207103469.1 glutathione synthase -
  ACNALX_RS02185 rsmE 431529..432260 (-) 732 WP_047887439.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACNALX_RS02190 - 432259..432522 (+) 264 WP_415720011.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38380.77 Da        Isoelectric Point: 6.3386

>NTDB_id=1170628 ACNALX_RS02160 WP_217394146.1 426753..427790(+) (pilT) [MAG: Photobacterium ganghwense isolate daa05ea6-4f3f-4f7c-82d4-3ed073323da5]
MDITELLDFSVKHNASDLHLSAGVPPMIRVDGDVRKLSLPSLEHSEVHRLVFDIMNDAQRREFEEKLEVDFSFELPDVGR
FRVNAFHQSRGCAAVFRTIPVHVPTLESLKVPDIFYQIAQCQRGLVLVTGATGSGKSTTIAALVDYINENYNRHILTIED
PVEFVHHGKRCLINQREVHRDTHSFHNALRSSLREDPDVIVVGELRDQETISLALTAAETGHLVLGTLHTSSAAKTVDRI
IDVFPGNDKAMVRSMLSESLRAVVSQSLLKCTSGGRIAAHEVMMATPAIRNLIREDKVAQMYSMIQTGSSVGMQTMEQSV
RMLVAQGQVEAEEGRRVVDNAFAQF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1170628 ACNALX_RS02160 WP_217394146.1 426753..427790(+) (pilT) [MAG: Photobacterium ganghwense isolate daa05ea6-4f3f-4f7c-82d4-3ed073323da5]
ATGGATATCACCGAACTACTGGACTTTAGTGTAAAGCATAATGCCTCGGATCTGCACCTCTCTGCGGGAGTTCCGCCGAT
GATTCGGGTAGATGGCGATGTTCGTAAACTGAGCTTGCCATCACTGGAGCACAGTGAAGTACACCGCTTGGTGTTTGACA
TTATGAACGATGCCCAGCGGCGCGAGTTTGAAGAAAAGCTCGAAGTCGATTTCTCGTTCGAGCTGCCGGACGTCGGGCGG
TTTCGTGTGAATGCCTTTCATCAGTCACGGGGATGCGCAGCGGTTTTCCGAACCATCCCGGTGCATGTGCCGACACTGGA
ATCACTGAAAGTGCCGGACATATTCTATCAGATCGCCCAATGCCAGCGCGGGCTGGTGCTGGTTACCGGGGCAACCGGCT
CCGGTAAATCCACCACGATTGCTGCGCTGGTCGATTACATCAATGAAAACTATAACCGCCATATCCTGACGATTGAAGAT
CCGGTGGAGTTTGTTCACCACGGCAAACGTTGCCTGATTAACCAGCGTGAAGTACACCGCGATACCCACAGTTTCCACAA
TGCACTGCGATCTTCCCTGCGTGAAGACCCGGATGTGATTGTGGTGGGGGAATTGCGCGATCAGGAAACCATCAGTCTGG
CGCTGACCGCGGCAGAAACCGGCCACCTTGTGCTGGGCACTCTGCACACCAGCTCGGCGGCCAAAACCGTCGATCGGATC
ATTGATGTCTTCCCGGGGAACGACAAGGCGATGGTGCGTTCTATGTTGTCTGAGTCGCTGCGGGCGGTGGTATCGCAGAG
CCTGCTTAAATGTACCAGCGGAGGCCGAATTGCGGCTCATGAAGTCATGATGGCGACCCCGGCTATCCGTAACCTGATCC
GCGAAGACAAAGTGGCCCAGATGTATTCCATGATCCAGACCGGCTCATCCGTCGGGATGCAGACCATGGAGCAAAGTGTC
CGGATGCTGGTGGCTCAGGGGCAGGTTGAGGCGGAAGAAGGACGCCGGGTCGTCGACAACGCGTTTGCGCAGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

77.477

96.522

0.748
  pilT Vibrio cholerae strain A1552

77.477

96.522

0.748
  pilT Acinetobacter baumannii D1279779

70.448

97.101

0.684
  pilT Acinetobacter baumannii strain A118

70.448

97.101

0.684
  pilT Acinetobacter nosocomialis M2

70.149

97.101

0.681
  pilT Acinetobacter baylyi ADP1

68.546

97.681

0.67
  pilT Pseudomonas aeruginosa PAK

68.657

97.101

0.667
  pilT Pseudomonas stutzeri DSM 10701

67.463

97.101

0.655
  pilT Legionella pneumophila strain Lp02

67.89

94.783

0.643
  pilT Legionella pneumophila strain ERS1305867

67.89

94.783

0.643
  pilT Neisseria meningitidis 8013

64.035

99.13

0.635
  pilT Neisseria gonorrhoeae MS11

63.743

99.13

0.632
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.974

98.841

0.484
  pilU Vibrio cholerae strain A1552

41.228

99.13

0.409
  pilU Pseudomonas stutzeri DSM 10701

41.916

96.812

0.406
  pilU Acinetobacter baylyi ADP1

39.13

93.333

0.365

Multiple sequence alignment