Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACNALX_RS02165 Genome accession   NZ_OZ223210
Coordinates   427815..428921 (+) Length   368 a.a.
NCBI ID   WP_047887443.1    Uniprot ID   A0A0J1GZT4
Organism   MAG: Photobacterium ganghwense isolate daa05ea6-4f3f-4f7c-82d4-3ed073323da5     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 422815..433921
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNALX_RS02130 - 422827..423420 (-) 594 WP_415720010.1 XTP/dITP diphosphatase -
  ACNALX_RS02135 - 423513..423950 (-) 438 WP_047887449.1 DUF4426 domain-containing protein -
  ACNALX_RS02140 yggU 424084..424377 (-) 294 WP_273857529.1 DUF167 family protein YggU -
  ACNALX_RS02145 - 424374..424928 (-) 555 WP_047887447.1 YggT family protein -
  ACNALX_RS02150 proC 424942..425760 (-) 819 WP_273857528.1 pyrroline-5-carboxylate reductase -
  ACNALX_RS02155 - 426013..426729 (-) 717 WP_273857527.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACNALX_RS02160 pilT 426753..427790 (+) 1038 WP_217394146.1 type IV pilus twitching motility protein PilT Machinery gene
  ACNALX_RS02165 pilU 427815..428921 (+) 1107 WP_047887443.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACNALX_RS02170 ruvX 429426..429854 (-) 429 WP_217394142.1 Holliday junction resolvase RuvX -
  ACNALX_RS02175 - 429851..430417 (-) 567 WP_047887441.1 YqgE/AlgH family protein -
  ACNALX_RS02180 gshB 430548..431498 (-) 951 WP_207103469.1 glutathione synthase -
  ACNALX_RS02185 rsmE 431529..432260 (-) 732 WP_047887439.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACNALX_RS02190 - 432259..432522 (+) 264 WP_415720011.1 hypothetical protein -
  ACNALX_RS02195 endA 432504..433208 (-) 705 WP_415720012.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41183.25 Da        Isoelectric Point: 6.1452

>NTDB_id=1170629 ACNALX_RS02165 WP_047887443.1 427815..428921(+) (pilU) [MAG: Photobacterium ganghwense isolate daa05ea6-4f3f-4f7c-82d4-3ed073323da5]
MTLQDALNEMVSSKASDLYITVEAPCLLKIDGNLHPLGQALDRPAIDRMFSEAMDDALYQEFCATKEANFALVRGEYRFR
VSAFWQRELPGMVIRRIETDIPDIFALNLPMDMQKMALAKRGLVLVVGATGSGKSTSLAAMTGYRNANRSGHILTVEDPI
EFVHKHNKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRTRETMEYAMNFAETGHLCMATLHANNANQALERILH
LVPKERRDQFLFDLSLNLKCILAQQLIPDAHGMGRHGAFELLINTPRVADLIRRGELHEIKDTMAKSAQSGMMTFDQSLY
DLYSAGKITEQDALHHADSANDLRLMIKVGNKHHQANSSLDGVTIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=1170629 ACNALX_RS02165 WP_047887443.1 427815..428921(+) (pilU) [MAG: Photobacterium ganghwense isolate daa05ea6-4f3f-4f7c-82d4-3ed073323da5]
ATGACATTACAGGATGCGCTGAATGAAATGGTCAGCAGCAAAGCCTCCGATTTGTACATCACGGTGGAAGCGCCATGTCT
GCTGAAAATCGACGGTAACCTGCACCCGCTGGGGCAGGCGCTGGATCGCCCGGCGATTGACCGGATGTTCAGCGAAGCCA
TGGACGATGCGTTGTATCAGGAGTTTTGTGCCACTAAAGAAGCAAACTTTGCGCTGGTACGCGGCGAGTATCGGTTCCGG
GTCAGTGCCTTCTGGCAGCGGGAACTGCCGGGCATGGTGATCCGGCGGATTGAAACCGATATCCCTGACATTTTTGCCCT
TAACTTGCCGATGGACATGCAGAAGATGGCACTGGCGAAACGGGGGCTGGTGCTGGTGGTCGGGGCGACCGGCTCGGGCA
AATCTACGTCACTGGCGGCGATGACCGGCTACCGTAATGCCAACCGTAGCGGTCACATTCTGACGGTGGAAGATCCGATT
GAGTTCGTGCACAAGCACAATAAGTGCATCGTGACCCAGCGCGAAGTCGGCCTGGATACGGAGAGCTACGAGGTGGCGCT
GAAGAACTCGCTGCGCCAGGCTCCTGACATGATTCTGATTGGCGAAATCCGGACCCGGGAAACCATGGAATATGCCATGA
ACTTTGCCGAAACCGGGCACCTGTGTATGGCGACCTTGCATGCCAATAATGCCAACCAGGCGTTGGAGCGTATTCTCCAT
CTGGTTCCGAAAGAGCGGCGGGATCAGTTTCTGTTCGATTTATCACTGAACCTGAAATGTATTCTGGCTCAGCAGCTGAT
CCCGGATGCCCACGGTATGGGACGGCACGGTGCCTTTGAGCTGTTGATCAACACGCCACGGGTTGCGGATCTGATCCGTC
GGGGTGAGCTGCACGAAATCAAGGACACCATGGCGAAATCAGCCCAGTCGGGGATGATGACGTTTGATCAGTCTCTGTAT
GACCTCTATAGCGCCGGCAAGATCACGGAGCAGGACGCTTTGCACCATGCCGATTCGGCCAACGATCTGCGACTGATGAT
TAAAGTCGGCAACAAACATCATCAGGCAAACAGCAGCTTGGATGGGGTCACGATCGATATGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(109-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0J1GZT4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

69.837

100

0.698
  pilU Pseudomonas stutzeri DSM 10701

58.221

100

0.587
  pilU Acinetobacter baylyi ADP1

54.795

99.185

0.543
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.653

91.576

0.372

Multiple sequence alignment