Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACFEL6_RS20885 Genome accession   NZ_OZ183475
Coordinates   3094263..3095300 (+) Length   345 a.a.
NCBI ID   WP_375321765.1    Uniprot ID   -
Organism   Aliivibrio logei isolate 622f83115ee5131b54a8b4d1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3089263..3100300
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFEL6_RS20850 hemW 3089469..3090629 (-) 1161 WP_375321759.1 radical SAM family heme chaperone HemW -
  ACFEL6_RS20855 - 3090633..3091229 (-) 597 WP_375321760.1 XTP/dITP diphosphatase -
  ACFEL6_RS20860 - 3091240..3091671 (-) 432 WP_105053991.1 DUF4426 domain-containing protein -
  ACFEL6_RS20865 yggU 3091727..3092026 (-) 300 WP_375321761.1 DUF167 family protein YggU -
  ACFEL6_RS20870 - 3092034..3092588 (-) 555 WP_375321762.1 YggT family protein -
  ACFEL6_RS20875 proC 3092651..3093469 (-) 819 WP_375321763.1 pyrroline-5-carboxylate reductase -
  ACFEL6_RS20880 - 3093529..3094239 (-) 711 WP_375321764.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACFEL6_RS20885 pilT 3094263..3095300 (+) 1038 WP_375321765.1 type IV pilus twitching motility protein PilT Machinery gene
  ACFEL6_RS20890 pilU 3095303..3096409 (+) 1107 WP_375322844.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACFEL6_RS20895 ruvX 3096549..3096974 (-) 426 WP_060992489.1 Holliday junction resolvase RuvX -
  ACFEL6_RS20900 - 3096971..3097534 (-) 564 WP_060992745.1 YqgE/AlgH family protein -
  ACFEL6_RS20905 gshB 3097600..3098547 (-) 948 WP_061004609.1 glutathione synthase -
  ACFEL6_RS20910 rsmE 3098561..3099292 (-) 732 WP_375321766.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACFEL6_RS20915 - 3099371..3100075 (-) 705 WP_375321767.1 endonuclease -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38182.80 Da        Isoelectric Point: 6.5763

>NTDB_id=1168064 ACFEL6_RS20885 WP_375321765.1 3094263..3095300(+) (pilT) [Aliivibrio logei isolate 622f83115ee5131b54a8b4d1]
MDITELLDFSVKQNASDLHLSAGVPPMVRVDGEVRKLTLPDFTHAEVHRLVTDIMNDAQRSEFEEKLEVDFSFELPNVGR
FRVNAFNQSRGCAAVFRTIPVEIPSLERLSAPEIFTTISNYQKGLVLITGPTGSGKSTTLAAMVDYINENHNKHVLTIED
PIEFVHKNKKCLINQREVHRDTHSFKAALRSALREDPDVILVGELRDKETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKSMVRSMLSESLRAVVAQTLLKRTGGGRVACHEIMIATPAIRNLIREDKVAQMYSIIQTGSSFGMQTMEQNA
KKIIAQGLVDPDEVAKKVEITSMGF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1168064 ACFEL6_RS20885 WP_375321765.1 3094263..3095300(+) (pilT) [Aliivibrio logei isolate 622f83115ee5131b54a8b4d1]
ATGGACATCACAGAACTACTGGACTTTAGTGTAAAACAAAACGCATCAGATCTACACCTATCTGCAGGAGTTCCTCCTAT
GGTACGTGTTGATGGTGAAGTGCGTAAACTGACATTACCTGATTTTACACACGCAGAGGTTCATCGTTTAGTGACTGACA
TTATGAATGATGCTCAGCGCAGTGAGTTTGAAGAGAAGTTAGAAGTCGATTTTTCATTTGAACTGCCTAATGTTGGGCGT
TTTCGTGTGAATGCATTTAATCAATCGAGAGGCTGTGCTGCGGTATTTAGAACTATTCCGGTAGAGATCCCATCATTAGA
GCGACTTTCTGCTCCTGAAATTTTCACAACCATTTCTAACTATCAAAAAGGTTTAGTGTTAATCACTGGGCCTACAGGGT
CAGGTAAATCCACCACGCTTGCTGCTATGGTGGATTACATTAATGAGAATCATAATAAGCATGTATTAACGATTGAGGAT
CCGATTGAGTTCGTACATAAAAATAAAAAATGTTTGATTAACCAACGTGAAGTACATCGTGACACTCATAGCTTTAAAGC
GGCACTACGCTCAGCATTACGTGAAGATCCTGATGTTATTTTAGTGGGTGAGCTTCGAGATAAAGAAACCATCAGTCTAG
CATTAACCGCCGCAGAAACCGGTCACTTAGTGTTTGGCACTTTGCATACTAGCTCAGCAGCAAAAACCATTGACCGTATT
ATAGATGTATTCCCTGGTAGTGATAAATCTATGGTTCGTTCTATGCTGTCGGAATCATTAAGAGCTGTTGTTGCTCAAAC
CTTATTAAAACGTACGGGTGGTGGTCGTGTCGCGTGCCATGAAATCATGATAGCAACGCCAGCTATCCGAAATTTAATTC
GAGAAGATAAAGTCGCTCAAATGTACTCTATCATTCAAACTGGATCTTCTTTTGGGATGCAGACGATGGAGCAAAATGCG
AAGAAAATTATTGCTCAGGGCTTAGTTGATCCTGATGAAGTCGCAAAGAAAGTTGAAATAACAAGCATGGGCTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

83.478

100

0.835
  pilT Vibrio cholerae strain A1552

83.478

100

0.835
  pilT Acinetobacter baumannii D1279779

72.81

95.942

0.699
  pilT Acinetobacter baumannii strain A118

72.81

95.942

0.699
  pilT Acinetobacter nosocomialis M2

72.508

95.942

0.696
  pilT Pseudomonas stutzeri DSM 10701

70.206

98.261

0.69
  pilT Pseudomonas aeruginosa PAK

69.412

98.551

0.684
  pilT Acinetobacter baylyi ADP1

71.515

95.652

0.684
  pilT Legionella pneumophila strain Lp02

68.997

95.362

0.658
  pilT Legionella pneumophila strain ERS1305867

68.997

95.362

0.658
  pilT Neisseria meningitidis 8013

65.68

97.971

0.643
  pilT Neisseria gonorrhoeae MS11

63.689

100

0.641
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.753

100

0.493
  pilU Pseudomonas stutzeri DSM 10701

41.566

96.232

0.4
  pilU Vibrio cholerae strain A1552

40.308

94.203

0.38
  pilU Acinetobacter baylyi ADP1

39.062

92.754

0.362

Multiple sequence alignment