Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACFEL6_RS20890 Genome accession   NZ_OZ183475
Coordinates   3095303..3096409 (+) Length   368 a.a.
NCBI ID   WP_375322844.1    Uniprot ID   -
Organism   Aliivibrio logei isolate 622f83115ee5131b54a8b4d1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3090303..3101409
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFEL6_RS20855 - 3090633..3091229 (-) 597 WP_375321760.1 XTP/dITP diphosphatase -
  ACFEL6_RS20860 - 3091240..3091671 (-) 432 WP_105053991.1 DUF4426 domain-containing protein -
  ACFEL6_RS20865 yggU 3091727..3092026 (-) 300 WP_375321761.1 DUF167 family protein YggU -
  ACFEL6_RS20870 - 3092034..3092588 (-) 555 WP_375321762.1 YggT family protein -
  ACFEL6_RS20875 proC 3092651..3093469 (-) 819 WP_375321763.1 pyrroline-5-carboxylate reductase -
  ACFEL6_RS20880 - 3093529..3094239 (-) 711 WP_375321764.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACFEL6_RS20885 pilT 3094263..3095300 (+) 1038 WP_375321765.1 type IV pilus twitching motility protein PilT Machinery gene
  ACFEL6_RS20890 pilU 3095303..3096409 (+) 1107 WP_375322844.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACFEL6_RS20895 ruvX 3096549..3096974 (-) 426 WP_060992489.1 Holliday junction resolvase RuvX -
  ACFEL6_RS20900 - 3096971..3097534 (-) 564 WP_060992745.1 YqgE/AlgH family protein -
  ACFEL6_RS20905 gshB 3097600..3098547 (-) 948 WP_061004609.1 glutathione synthase -
  ACFEL6_RS20910 rsmE 3098561..3099292 (-) 732 WP_375321766.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACFEL6_RS20915 - 3099371..3100075 (-) 705 WP_375321767.1 endonuclease -
  ACFEL6_RS20920 - 3100189..3100683 (-) 495 WP_375321768.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41379.45 Da        Isoelectric Point: 6.3423

>NTDB_id=1168065 ACFEL6_RS20890 WP_375322844.1 3095303..3096409(+) (pilU) [Aliivibrio logei isolate 622f83115ee5131b54a8b4d1]
MMLDELLHQMVKQKASDVYLTVDAPCLLRIHGELRAIGHKQTNESVSALLAEMMDEDRQTEFNQTLEANFAIVKESGRFR
VSAFWQREQPGVVIRRIETKIPSMESLHLPEVMKELAIAKRGLVLVVGATGSGKSTTMAAMTGYRNDNKSGHILTVEDPI
EFVHPHHKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKDQKEQFLFDLSMNLKGIIAQQLVPDINGHGRHGVYEVLLNTPRISDLIRRGELHELKATMTKSRESGMQTFDQCLF
DLVVANKISEDEALHSADSANDLRLMLKTKRGDSFTTSKFDNVSISLD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=1168065 ACFEL6_RS20890 WP_375322844.1 3095303..3096409(+) (pilU) [Aliivibrio logei isolate 622f83115ee5131b54a8b4d1]
ATGATGTTAGATGAATTACTACATCAAATGGTAAAACAAAAAGCATCGGATGTTTACTTAACTGTTGATGCTCCATGTTT
ATTGCGAATTCATGGTGAGCTTAGAGCGATTGGTCACAAGCAGACCAATGAATCTGTTTCTGCATTATTAGCGGAGATGA
TGGATGAAGATCGCCAGACTGAATTTAATCAAACATTAGAAGCTAACTTTGCCATTGTAAAAGAAAGTGGACGCTTTCGC
GTGAGTGCATTTTGGCAGCGTGAGCAACCAGGAGTGGTTATCCGTCGTATTGAAACTAAAATTCCATCCATGGAGTCATT
ACATTTACCTGAAGTGATGAAAGAGCTAGCGATAGCTAAGCGTGGCTTAGTTCTGGTTGTTGGCGCAACAGGCTCTGGTA
AATCGACAACGATGGCAGCAATGACAGGGTATCGTAATGATAATAAATCCGGTCATATATTAACAGTTGAAGATCCGATT
GAATTTGTTCACCCTCACCACAAATGCATCGTTACTCAGCGTGAAGTTGGCTTAGATACCGAAAGCTATGAAGTCGCGTT
AAAGAACTCTTTGCGACAGGCTCCTGACATGATTTTAATTGGTGAAATCCGCAGTCGAGAGACGATGGAGTACGCCATGA
CCTTTGCGGAGACGGGGCACCTTTGTATGGCAACGCTCCATGCTAATAATGCCAACCAAGCGTTAGAGCGAATTCTCCAC
TTAGTACCGAAAGATCAGAAAGAGCAGTTCTTATTTGACCTTTCTATGAACTTAAAAGGGATCATTGCTCAACAATTGGT
TCCTGATATAAATGGTCACGGGCGTCATGGCGTTTATGAGGTGCTGTTAAATACTCCTCGGATTTCGGATTTGATCCGTA
GAGGTGAGTTGCATGAATTAAAAGCTACCATGACAAAATCGCGAGAATCAGGCATGCAGACGTTTGATCAGTGTTTATTT
GATCTAGTGGTGGCAAATAAGATTTCGGAAGATGAGGCATTGCACAGTGCAGATTCTGCGAATGACTTGAGGTTGATGCT
GAAGACGAAGCGTGGTGATTCATTTACGACGTCGAAGTTTGATAATGTGTCGATATCGTTAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

76.087

100

0.761
  pilU Pseudomonas stutzeri DSM 10701

57.062

96.196

0.549
  pilU Acinetobacter baylyi ADP1

54.701

95.38

0.522
  pilT Pseudomonas aeruginosa PAK

42.274

93.207

0.394
  pilT Pseudomonas stutzeri DSM 10701

41.691

93.207

0.389
  pilT Legionella pneumophila strain Lp02

41.888

92.12

0.386
  pilT Legionella pneumophila strain ERS1305867

41.888

92.12

0.386
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.543

91.576

0.38
  pilT Vibrio cholerae strain A1552

42.947

86.685

0.372
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.947

86.685

0.372
  pilT Acinetobacter baumannii D1279779

40.413

92.12

0.372
  pilT Acinetobacter baumannii strain A118

40.413

92.12

0.372
  pilT Acinetobacter nosocomialis M2

40.413

92.12

0.372

Multiple sequence alignment