Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AB8516_RS04450 Genome accession   NZ_OZ156465
Coordinates   1026962..1027996 (-) Length   344 a.a.
NCBI ID   WP_108289743.1    Uniprot ID   -
Organism   Candidatus Thiodiazotropha sp. LNASS1 isolate 6c41e2f9-faec-415b-97c3-6ddcd12de2e9     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1021962..1032996
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB8516_RS04425 - 1022076..1023683 (+) 1608 WP_369158463.1 TIR domain-containing protein -
  AB8516_RS04430 - 1024084..1024419 (+) 336 WP_201259016.1 DUF1820 family protein -
  AB8516_RS04435 - 1024490..1025071 (+) 582 WP_369158466.1 ankyrin repeat domain-containing protein -
  AB8516_RS04440 - 1025227..1025691 (+) 465 WP_369158468.1 hypothetical protein -
  AB8516_RS04445 pilU 1025711..1026835 (-) 1125 WP_369158470.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AB8516_RS04450 pilT 1026962..1027996 (-) 1035 WP_108289743.1 type IV pilus twitching motility protein PilT Machinery gene
  AB8516_RS04455 - 1028550..1029257 (+) 708 WP_369158473.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB8516_RS04460 proC 1029295..1030122 (+) 828 WP_108289745.1 pyrroline-5-carboxylate reductase -
  AB8516_RS04465 - 1030122..1030700 (+) 579 WP_369158476.1 YggT family protein -
  AB8516_RS04470 - 1030697..1030981 (+) 285 WP_108289747.1 DUF167 family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38036.77 Da        Isoelectric Point: 6.7747

>NTDB_id=1167903 AB8516_RS04450 WP_108289743.1 1026962..1027996(-) (pilT) [Candidatus Thiodiazotropha sp. LNASS1 isolate 6c41e2f9-faec-415b-97c3-6ddcd12de2e9]
MDIAELLAFSVKHNASDLHISAGLPPMIRVDGDIRRINVPALEHKVVHALVYDIMNDKQRKDFEEFLENDFSFEIPGLAR
FRVNAFNQARGASAVFRTIPSKVLTLEELGCPQTFKDIVDVPRGLVLVTGPTGSGKSTTLAAMMDYKNDNDYSHILTIED
PIEFVHSSKKCLINQREVHRDTLGFSEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKSMVRSMLSESLRSVIAQTLLKKIGGGRIAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQAAGMQTMDQAL
KELVQRGVVSRLDAKAKAQNKDQF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1167903 AB8516_RS04450 WP_108289743.1 1026962..1027996(-) (pilT) [Candidatus Thiodiazotropha sp. LNASS1 isolate 6c41e2f9-faec-415b-97c3-6ddcd12de2e9]
ATGGATATCGCTGAACTTCTGGCCTTCAGTGTCAAGCATAACGCATCAGATTTGCATATATCTGCCGGTTTGCCGCCCAT
GATTCGTGTGGATGGCGACATCAGGCGCATCAATGTCCCCGCACTTGAACACAAGGTCGTGCACGCGCTTGTGTATGACA
TCATGAATGATAAACAGCGCAAGGACTTCGAGGAGTTCCTTGAGAACGACTTTTCCTTCGAGATCCCCGGATTGGCGAGA
TTTCGTGTCAATGCCTTCAATCAGGCACGCGGGGCCTCGGCTGTTTTTCGCACCATCCCATCCAAGGTGTTGACGCTGGA
AGAGCTCGGTTGTCCTCAAACCTTCAAGGATATCGTGGATGTACCCCGGGGACTGGTGTTGGTGACCGGACCGACCGGTT
CCGGTAAGTCGACCACGCTTGCCGCCATGATGGATTACAAGAACGACAATGACTACTCCCATATCCTCACCATCGAGGAT
CCGATCGAGTTTGTTCACTCCAGTAAGAAGTGCCTGATCAACCAGCGCGAAGTGCATCGTGACACTCTGGGATTTTCCGA
GGCGCTGCGTTCCGCATTGCGTGAAGATCCGGATATCATCCTGGTCGGCGAGCTGCGTGATCTGGAAACCATCCGCCTGG
CGTTGACTGCCGCCGAGACCGGCCACTTGGTATTCGGTACCCTGCACACCAGTTCGGCGGCCAAGACCATCGACCGTATC
ATTGACGTATTTCCGGCGGGGGAGAAGTCGATGGTGCGCTCGATGCTCTCCGAATCGCTGCGATCCGTTATTGCGCAAAC
TCTGCTGAAAAAGATTGGCGGCGGACGAATTGCGGCGTGGGAGATCATGGTCGGCACACCCGCGATACGTAACCTGATCC
GTGAGGACAAGGTTGCCCAGATGTATTCCGCGATTCAGACCGGCCAGGCGGCGGGTATGCAGACCATGGACCAGGCACTG
AAGGAGTTGGTGCAGCGCGGAGTTGTAAGCCGTCTTGATGCCAAGGCCAAGGCACAAAATAAGGATCAGTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

81.686

100

0.817
  pilT Pseudomonas stutzeri DSM 10701

80.523

100

0.805
  pilT Acinetobacter nosocomialis M2

79.36

100

0.794
  pilT Acinetobacter baumannii D1279779

79.36

100

0.794
  pilT Acinetobacter baumannii strain A118

79.36

100

0.794
  pilT Acinetobacter baylyi ADP1

76.744

100

0.767
  pilT Legionella pneumophila strain Lp02

75

100

0.75
  pilT Legionella pneumophila strain ERS1305867

75

100

0.75
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.271

98.547

0.712
  pilT Vibrio cholerae strain A1552

72.271

98.547

0.712
  pilT Neisseria meningitidis 8013

65.889

99.709

0.657
  pilT Neisseria gonorrhoeae MS11

65.598

99.709

0.654
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.435

100

0.506
  pilU Vibrio cholerae strain A1552

42.388

97.384

0.413
  pilU Pseudomonas stutzeri DSM 10701

41.108

99.709

0.41
  pilU Acinetobacter baylyi ADP1

39.412

98.837

0.39

Multiple sequence alignment