Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AB8516_RS04445 Genome accession   NZ_OZ156465
Coordinates   1025711..1026835 (-) Length   374 a.a.
NCBI ID   WP_369158470.1    Uniprot ID   -
Organism   Candidatus Thiodiazotropha sp. LNASS1 isolate 6c41e2f9-faec-415b-97c3-6ddcd12de2e9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1020711..1031835
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB8516_RS04420 - 1021049..1021768 (+) 720 WP_369158461.1 hypothetical protein -
  AB8516_RS04425 - 1022076..1023683 (+) 1608 WP_369158463.1 TIR domain-containing protein -
  AB8516_RS04430 - 1024084..1024419 (+) 336 WP_201259016.1 DUF1820 family protein -
  AB8516_RS04435 - 1024490..1025071 (+) 582 WP_369158466.1 ankyrin repeat domain-containing protein -
  AB8516_RS04440 - 1025227..1025691 (+) 465 WP_369158468.1 hypothetical protein -
  AB8516_RS04445 pilU 1025711..1026835 (-) 1125 WP_369158470.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AB8516_RS04450 pilT 1026962..1027996 (-) 1035 WP_108289743.1 type IV pilus twitching motility protein PilT Machinery gene
  AB8516_RS04455 - 1028550..1029257 (+) 708 WP_369158473.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB8516_RS04460 proC 1029295..1030122 (+) 828 WP_108289745.1 pyrroline-5-carboxylate reductase -
  AB8516_RS04465 - 1030122..1030700 (+) 579 WP_369158476.1 YggT family protein -
  AB8516_RS04470 - 1030697..1030981 (+) 285 WP_108289747.1 DUF167 family protein -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 41827.06 Da        Isoelectric Point: 6.5671

>NTDB_id=1167902 AB8516_RS04445 WP_369158470.1 1025711..1026835(-) (pilU) [Candidatus Thiodiazotropha sp. LNASS1 isolate 6c41e2f9-faec-415b-97c3-6ddcd12de2e9]
MDLNALLSVVVKNKASDLFITAGREPSIKVDGKIHPINKTPLTPRETKEMVYSIMTDAQQKEFDETRECNFAISAKSLGR
FRVSSFYQRDTVGMVLRRIETKIPSLESLYLPAILQDLSMVKRGLVIFVGATGTGKSTSLAAMIGYRNTRGEGHIVSIED
PIEYMHDHDKCIITQREVGIDTESYEIALKNTLRQAPDVILIGEIRTRKTMEHAIAFAETGHLCLSTLHANNANQALDRI
IHFFPEDARDQIFMDLSLNLKAIVAQQLIPRVDGNGRRAAVEVMLNTPLAAELIRKGEIHKLKELMKRSTEHGMITFDQH
LFQLYEEGVISYENALAHADSANDVRLMIKLGATHTSDSLIDELDGITLSDGKR

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=1167902 AB8516_RS04445 WP_369158470.1 1025711..1026835(-) (pilU) [Candidatus Thiodiazotropha sp. LNASS1 isolate 6c41e2f9-faec-415b-97c3-6ddcd12de2e9]
ATGGATTTGAACGCACTCCTGTCCGTGGTCGTCAAAAACAAGGCGTCCGATCTGTTCATAACCGCCGGGCGGGAACCCTC
CATCAAGGTGGATGGAAAGATCCATCCGATCAACAAGACCCCTCTGACACCCCGAGAGACCAAGGAGATGGTCTACAGCA
TCATGACCGATGCGCAACAGAAGGAGTTCGACGAAACCAGAGAGTGCAATTTCGCCATCAGTGCGAAAAGCCTGGGGCGT
TTCCGCGTCAGCAGCTTTTATCAGCGCGATACCGTGGGCATGGTGTTACGTCGCATCGAAACCAAGATACCCAGCCTGGA
GTCACTCTACCTGCCCGCCATTCTGCAGGATCTCTCCATGGTGAAGCGTGGCCTGGTGATCTTCGTGGGCGCCACCGGTA
CCGGCAAGTCGACCTCTCTGGCTGCCATGATCGGCTATCGGAATACCAGGGGCGAGGGCCATATCGTCAGTATCGAGGAC
CCCATCGAGTATATGCATGATCACGACAAGTGCATCATTACCCAGCGCGAAGTAGGCATAGATACCGAGTCCTATGAGAT
TGCGCTGAAGAACACCCTGCGCCAGGCGCCCGATGTGATCCTGATCGGTGAGATACGTACCCGTAAGACCATGGAACACG
CGATAGCCTTTGCCGAGACCGGCCATCTCTGCCTGTCGACCTTGCATGCCAACAACGCCAATCAGGCGCTTGATCGTATC
ATTCACTTCTTCCCGGAGGATGCCAGAGATCAGATCTTCATGGATCTCTCCCTGAATCTGAAGGCAATCGTGGCTCAGCA
ACTGATCCCGCGTGTGGACGGTAATGGTCGGCGGGCTGCCGTCGAGGTGATGTTGAATACCCCATTGGCCGCAGAACTGA
TTCGCAAGGGCGAAATCCACAAGCTCAAAGAGCTGATGAAGCGTTCAACGGAACACGGCATGATTACCTTTGATCAGCAT
CTCTTTCAGCTCTATGAAGAGGGTGTCATCAGCTATGAGAATGCCTTGGCCCATGCCGATTCCGCCAATGATGTACGCTT
GATGATCAAACTGGGCGCCACCCATACCAGTGACTCGCTGATCGATGAACTGGATGGAATCACCCTGTCGGATGGTAAAC
GCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.751

98.663

0.668
  pilU Acinetobacter baylyi ADP1

65.156

94.385

0.615
  pilU Vibrio cholerae strain A1552

57.429

93.583

0.537
  pilT Legionella pneumophila strain Lp02

42.985

89.572

0.385
  pilT Legionella pneumophila strain ERS1305867

42.985

89.572

0.385
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.183

90.642

0.382
  pilT Acinetobacter baylyi ADP1

41.642

91.176

0.38
  pilT Vibrio cholerae strain A1552

42.121

88.235

0.372
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.121

88.235

0.372
  pilT Acinetobacter nosocomialis M2

41.194

89.572

0.369
  pilT Acinetobacter baumannii D1279779

41.194

89.572

0.369
  pilT Acinetobacter baumannii strain A118

41.194

89.572

0.369
  pilT Pseudomonas aeruginosa PAK

40.597

89.572

0.364

Multiple sequence alignment