Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AB5Y62_RS02225 Genome accession   NZ_OZ078860
Coordinates   452648..453757 (+) Length   369 a.a.
NCBI ID   WP_029406451.1    Uniprot ID   -
Organism   Vibrio splendidus isolate A06     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 447648..458757
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB5Y62_RS02190 - 447873..448472 (-) 600 WP_368084092.1 XTP/dITP diphosphatase -
  AB5Y62_RS02195 - 448521..448952 (-) 432 WP_004735472.1 DUF4426 domain-containing protein -
  AB5Y62_RS02200 yggU 448981..449271 (-) 291 WP_054541895.1 DUF167 family protein YggU -
  AB5Y62_RS02205 - 449301..449858 (-) 558 WP_004735475.1 YggT family protein -
  AB5Y62_RS02210 proC 449934..450752 (-) 819 WP_054541894.1 pyrroline-5-carboxylate reductase -
  AB5Y62_RS02215 - 450865..451569 (-) 705 WP_054541893.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB5Y62_RS02220 pilT 451596..452633 (+) 1038 WP_004735478.1 type IV pilus twitching motility protein PilT Machinery gene
  AB5Y62_RS02225 pilU 452648..453757 (+) 1110 WP_029406451.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AB5Y62_RS02230 - 453864..454025 (-) 162 WP_017090793.1 hypothetical protein -
  AB5Y62_RS02235 ruvX 454124..454546 (-) 423 WP_004735480.1 Holliday junction resolvase RuvX -
  AB5Y62_RS02240 - 454614..455177 (-) 564 WP_017095267.1 YqgE/AlgH family protein -
  AB5Y62_RS02245 gshB 455294..456244 (-) 951 WP_004735482.1 glutathione synthase -
  AB5Y62_RS02250 rsmE 456254..456985 (-) 732 WP_102489086.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AB5Y62_RS02255 - 457145..457876 (-) 732 WP_102489085.1 endonuclease -
  AB5Y62_RS02260 - 457974..458474 (-) 501 WP_368084093.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41323.24 Da        Isoelectric Point: 6.0660

>NTDB_id=1166574 AB5Y62_RS02225 WP_029406451.1 452648..453757(+) (pilU) [Vibrio splendidus isolate A06]
MELNQILEGMLSQKASDLYITVDAPILFRVDGELRPQGEKLNAAQVAQLLDAMMDQDRRDEYQQTREANFAIVRDFGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPDVLQDLSIAKRGLVLVVGATGSGKSTSMAAMTGYRNTNRSGHILTVEDPI
EFVHEHKKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLRGVVAQQLIRDKNGSGRHGVFEILLNSPRVSDLIRRGELHELKATMAKSKEIGMQTFDQALY
DLVLAGKISEEDAFHSADSANDLRLMLKTRRGGDDYGTGTLAGVKIDMG

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=1166574 AB5Y62_RS02225 WP_029406451.1 452648..453757(+) (pilU) [Vibrio splendidus isolate A06]
ATGGAATTGAATCAAATCCTTGAAGGGATGCTATCTCAAAAAGCATCGGATCTTTATATCACCGTTGATGCGCCTATCCT
GTTTCGTGTCGATGGTGAACTGCGACCTCAAGGCGAGAAATTGAATGCGGCTCAGGTTGCTCAGTTACTTGATGCGATGA
TGGATCAAGACCGACGCGATGAATATCAGCAAACGCGAGAGGCTAATTTTGCCATCGTACGTGATTTTGGTCGTTTTCGT
GTGAGTGCGTTTTTTCAGCGAGAGCTACCAGGAGCGGTCATTCGTCGTATCGAAACTAACATCCCAACCTTTGAGCAATT
AAAGCTTCCTGATGTGCTGCAAGACCTTTCAATCGCTAAGCGCGGACTTGTGCTGGTGGTTGGGGCTACAGGTTCTGGTA
AATCAACCTCGATGGCCGCAATGACAGGCTATCGCAATACCAATCGCTCAGGGCATATTTTGACGGTTGAAGATCCGATT
GAATTCGTACACGAACATAAAAAGTGCATCGTGACACAACGTGAGGTCGGGCTCGACACCGAGAGTTACGAAGTTGCGCT
TAAGAACTCTTTGCGCCAAGCTCCTGATATGATTTTGATTGGCGAGATCCGTAGCCGTGAAACCATGGAATACGCGATGA
CCTTTGCTGAAACGGGTCATTTGTGTATGGCAACACTGCACGCCAATAATGCCAACCAAGCGTTAGAACGTATTCTTCAC
TTGGTGCCGAAAGAGCAAAAAGAGCAATTCTTGTTTGATCTGTCGATGAACCTGCGTGGTGTGGTCGCTCAGCAATTAAT
CCGAGATAAGAATGGCAGCGGTCGCCATGGCGTGTTTGAGATTCTACTTAACAGCCCGCGAGTGTCTGACTTGATTCGTC
GTGGTGAGTTGCATGAACTAAAAGCGACTATGGCTAAATCGAAAGAGATTGGTATGCAGACCTTTGACCAAGCGTTGTAT
GACTTGGTGCTTGCAGGCAAGATCAGTGAAGAAGACGCTTTCCACAGTGCTGATTCTGCCAATGATTTGCGCTTAATGCT
AAAGACTAGGCGTGGTGGTGATGACTATGGAACTGGTACGTTGGCTGGCGTTAAGATTGATATGGGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

84.511

99.729

0.843
  pilU Pseudomonas stutzeri DSM 10701

58.286

94.851

0.553
  pilU Acinetobacter baylyi ADP1

54.416

95.122

0.518
  pilT Acinetobacter baumannii strain A118

41.813

92.683

0.388
  pilT Acinetobacter baumannii D1279779

41.813

92.683

0.388
  pilT Acinetobacter nosocomialis M2

41.813

92.683

0.388
  pilT Legionella pneumophila strain ERS1305867

42.183

91.87

0.388
  pilT Legionella pneumophila strain Lp02

42.183

91.87

0.388
  pilT Pseudomonas aeruginosa PAK

41.593

91.87

0.382
  pilT Acinetobacter baylyi ADP1

42.169

89.973

0.379
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.21

87.805

0.379
  pilT Pseudomonas stutzeri DSM 10701

40.299

90.786

0.366
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.419

90.515

0.366
  pilT Vibrio cholerae strain A1552

40.419

90.515

0.366
  pilT Neisseria gonorrhoeae MS11

38.841

93.496

0.363
  pilT Neisseria meningitidis 8013

38.841

93.496

0.363

Multiple sequence alignment