Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AB5Y62_RS02220 Genome accession   NZ_OZ078860
Coordinates   451596..452633 (+) Length   345 a.a.
NCBI ID   WP_004735478.1    Uniprot ID   A0A1R3EAA6
Organism   Vibrio splendidus isolate A06     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 446596..457633
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB5Y62_RS02185 hemW 446686..447873 (-) 1188 WP_017095270.1 radical SAM family heme chaperone HemW -
  AB5Y62_RS02190 - 447873..448472 (-) 600 WP_368084092.1 XTP/dITP diphosphatase -
  AB5Y62_RS02195 - 448521..448952 (-) 432 WP_004735472.1 DUF4426 domain-containing protein -
  AB5Y62_RS02200 yggU 448981..449271 (-) 291 WP_054541895.1 DUF167 family protein YggU -
  AB5Y62_RS02205 - 449301..449858 (-) 558 WP_004735475.1 YggT family protein -
  AB5Y62_RS02210 proC 449934..450752 (-) 819 WP_054541894.1 pyrroline-5-carboxylate reductase -
  AB5Y62_RS02215 - 450865..451569 (-) 705 WP_054541893.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB5Y62_RS02220 pilT 451596..452633 (+) 1038 WP_004735478.1 type IV pilus twitching motility protein PilT Machinery gene
  AB5Y62_RS02225 pilU 452648..453757 (+) 1110 WP_029406451.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AB5Y62_RS02230 - 453864..454025 (-) 162 WP_017090793.1 hypothetical protein -
  AB5Y62_RS02235 ruvX 454124..454546 (-) 423 WP_004735480.1 Holliday junction resolvase RuvX -
  AB5Y62_RS02240 - 454614..455177 (-) 564 WP_017095267.1 YqgE/AlgH family protein -
  AB5Y62_RS02245 gshB 455294..456244 (-) 951 WP_004735482.1 glutathione synthase -
  AB5Y62_RS02250 rsmE 456254..456985 (-) 732 WP_102489086.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38317.83 Da        Isoelectric Point: 6.0512

>NTDB_id=1166573 AB5Y62_RS02220 WP_004735478.1 451596..452633(+) (pilT) [Vibrio splendidus isolate A06]
MDITELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLGIPALSHADVHRLVFEIMSDSQRGEFEEKLEVDFSFELPNVGR
FRVNAFNQSRGCSAVFRTIPVEIPTLEQLGAPEIFERISNYEKGLVLVTGPTGSGKSTTLAAMVDYVNRNHNKHILTIED
PIEFVHTNNKCLINQREVHRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRSVIAQKLLKRVGGGRVACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLMAQGLVDSEEVEKKIEIETSMF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1166573 AB5Y62_RS02220 WP_004735478.1 451596..452633(+) (pilT) [Vibrio splendidus isolate A06]
ATGGATATCACTGAGTTACTAGATTTTAGTGTAAAGCATAACGCGTCAGATCTACATCTTTCTGCGGGTGTATCTCCAAT
GGTACGTATAGATGGTGAAGTAAGGAAGCTTGGAATACCAGCTTTGAGCCATGCTGATGTGCATCGTTTGGTTTTTGAGA
TCATGAGTGATTCACAACGCGGTGAGTTCGAAGAAAAACTGGAAGTCGACTTCTCTTTTGAATTACCCAACGTTGGTCGC
TTCCGTGTTAATGCTTTTAACCAATCTCGTGGCTGCTCTGCTGTGTTCCGTACCATTCCAGTAGAGATTCCCACCCTTGA
ACAGCTAGGGGCTCCTGAGATATTTGAAAGAATATCTAATTATGAAAAAGGGCTAGTATTGGTCACAGGGCCGACTGGTT
CAGGTAAGTCGACAACTTTGGCTGCGATGGTGGATTACGTTAACCGTAACCATAATAAGCATATATTGACGATTGAAGAT
CCGATCGAATTTGTTCATACCAACAACAAATGCCTAATCAACCAACGAGAAGTGCATCGCGATACTCACAGCTTCAAAGC
GGCATTGCGTAGTGCGTTGCGTGAAGATCCAGACGTAATCCTTGTCGGCGAACTTCGTGACCAAGAGACGATCAGCTTAG
CGCTAACTGCAGCAGAAACGGGTCACTTAGTTTTTGGTACTTTGCACACGAGCTCTGCGGCAAAAACCATAGACCGTATT
ATCGATGTATTCCCGGGTAGCGACAAAGACATGGTGCGTTCAATGTTGTCTGAATCGTTACGTTCGGTGATTGCCCAGAA
GCTGTTGAAGCGTGTTGGTGGCGGTCGTGTGGCTTGTCATGAAATCATGATGGCGACACCTGCGATCAGGAACTTGATCC
GTGAAGACAAGGTCGCGCAGATGTATTCGATTATTCAAACGGGGGCGGCACACGGTATGCAGACCATGGAGCAAAATGCG
AAGCAGTTGATGGCTCAAGGTCTGGTTGATTCAGAAGAGGTCGAGAAAAAGATCGAAATTGAAACCTCAATGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1R3EAA6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

88.696

100

0.887
  pilT Vibrio cholerae strain A1552

88.696

100

0.887
  pilT Acinetobacter baumannii D1279779

72.59

96.232

0.699
  pilT Acinetobacter baumannii strain A118

72.59

96.232

0.699
  pilT Acinetobacter nosocomialis M2

72.289

96.232

0.696
  pilT Pseudomonas aeruginosa PAK

69.412

98.551

0.684
  pilT Acinetobacter baylyi ADP1

71.515

95.652

0.684
  pilT Pseudomonas stutzeri DSM 10701

69.139

97.681

0.675
  pilT Legionella pneumophila strain Lp02

65.507

100

0.655
  pilT Legionella pneumophila strain ERS1305867

65.507

100

0.655
  pilT Neisseria meningitidis 8013

62.609

100

0.626
  pilT Neisseria gonorrhoeae MS11

62.319

100

0.623
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.147

98.841

0.496
  pilU Vibrio cholerae strain A1552

42.769

94.203

0.403
  pilU Pseudomonas stutzeri DSM 10701

40.12

96.812

0.388
  pilU Acinetobacter baylyi ADP1

39.688

92.754

0.368

Multiple sequence alignment