Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   YC6258_RS03795 Genome accession   NZ_CP007142
Coordinates   854394..855428 (+) Length   344 a.a.
NCBI ID   WP_044615869.1    Uniprot ID   A0A0C5VHT4
Organism   Gynuella sunshinyii YC6258     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 849394..860428
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  YC6258_RS03760 (YC6258_00866) - 849602..850048 (-) 447 WP_044615862.1 DUF4426 domain-containing protein -
  YC6258_RS03765 (YC6258_00867) metW 850045..850638 (-) 594 WP_044615863.1 methionine biosynthesis protein MetW -
  YC6258_RS03770 (YC6258_00868) - 850638..851786 (-) 1149 WP_044615864.1 homoserine O-acetyltransferase -
  YC6258_RS03775 (YC6258_00869) - 851801..852109 (-) 309 WP_044615865.1 DUF167 family protein -
  YC6258_RS03780 (YC6258_00870) - 852106..852693 (-) 588 WP_044615866.1 YggT family protein -
  YC6258_RS03785 (YC6258_00871) proC 852693..853523 (-) 831 WP_044615867.1 pyrroline-5-carboxylate reductase -
  YC6258_RS03790 (YC6258_00872) - 853543..854232 (-) 690 WP_044615868.1 YggS family pyridoxal phosphate-dependent enzyme -
  YC6258_RS03795 (YC6258_00873) pilT 854394..855428 (+) 1035 WP_044615869.1 type IV pilus twitching motility protein PilT Machinery gene
  YC6258_RS03800 (YC6258_00874) pilU 855468..856595 (+) 1128 WP_044615870.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  YC6258_RS03805 - 857052..857486 (+) 435 WP_044615871.1 hypothetical protein -
  YC6258_RS03810 (YC6258_00877) hemH 857527..858558 (-) 1032 WP_044615872.1 ferrochelatase -
  YC6258_RS03815 (YC6258_00878) - 858652..859275 (-) 624 WP_082070547.1 LysE family translocator -
  YC6258_RS03820 (YC6258_00879) glpE 859272..859568 (-) 297 WP_044619702.1 thiosulfate sulfurtransferase GlpE -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38208.84 Da        Isoelectric Point: 6.7250

>NTDB_id=116466 YC6258_RS03795 WP_044615869.1 854394..855428(+) (pilT) [Gynuella sunshinyii YC6258]
MDITELLAFSAKQNASDLHISAGLPPMIRVDGDVRRINLPAMGHKEVHALIYDIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGQVFKDVCHNPRGLVLVTGPTGSGKSTSLAAMINYINESRYEHILTIED
PIEFVHESKKCLVNQREVHRDTHGFSEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKSMVRSMLSESLQAVISQTLLKKNGGGRIAAHEIMIGTPAIRNLVREDKVAQMYSAIQTGANVGMQTLDQCL
KGLLQRGMITREIARERAKQPESF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=116466 YC6258_RS03795 WP_044615869.1 854394..855428(+) (pilT) [Gynuella sunshinyii YC6258]
ATGGATATCACTGAATTATTGGCTTTTAGTGCCAAACAAAACGCATCTGACTTGCATATTTCGGCGGGGTTGCCACCGAT
GATCAGGGTTGATGGCGACGTTCGTCGAATCAATCTGCCGGCCATGGGGCATAAAGAAGTGCATGCGTTGATTTATGACA
TCATGAATGACAAACAGCGTAAGGACTATGAGGAGTTTCTGGAAACTGACTTCTCGTTTGAAGTGCCGGGAGTCGCCCGT
TTCCGGGTAAACGCATTTAATCAGAACCGTGGTGCAGGTGCGGTGTTCCGGACCATTCCATCTAAGGTTTTGACCATGGA
AGACCTTGGCATGGGGCAGGTTTTTAAGGATGTCTGTCATAATCCGCGTGGCCTGGTGCTGGTGACCGGGCCAACCGGTT
CTGGTAAATCCACCTCTCTGGCGGCGATGATCAACTATATCAATGAAAGCCGTTATGAGCACATTCTGACCATTGAAGAT
CCAATAGAATTTGTGCATGAATCCAAAAAATGTCTGGTGAATCAGCGGGAAGTACACCGCGATACCCACGGATTCAGTGA
AGCTTTGCGTTCGGCACTGCGGGAAGACCCCGACATTATCCTGGTGGGGGAATTACGGGACCTGGAAACCATTCGTCTGG
CGTTGACTGCGGCAGAAACCGGCCACCTGGTATTTGGTACTCTGCACACCACCTCGGCAGCCAAGACCATTGACCGGGTG
ATCGACGTTTTCCCGGCCGAGGAAAAATCCATGGTGCGGTCCATGTTGTCTGAATCCCTGCAGGCCGTTATCTCGCAAAC
TCTGTTGAAAAAGAACGGTGGTGGCCGGATTGCCGCCCATGAAATTATGATCGGTACTCCGGCGATTCGTAACCTGGTGC
GTGAGGATAAGGTTGCGCAGATGTACTCGGCCATACAAACGGGGGCCAATGTCGGTATGCAGACTCTGGATCAGTGTCTG
AAAGGCCTGCTGCAGCGTGGCATGATTACCCGCGAAATCGCACGCGAACGGGCCAAACAGCCTGAATCTTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0C5VHT4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

88.081

100

0.881
  pilT Pseudomonas aeruginosa PAK

87.5

100

0.875
  pilT Acinetobacter nosocomialis M2

86.047

100

0.86
  pilT Acinetobacter baumannii D1279779

86.047

100

0.86
  pilT Acinetobacter baumannii strain A118

86.047

100

0.86
  pilT Acinetobacter baylyi ADP1

82.267

100

0.823
  pilT Legionella pneumophila strain Lp02

74.709

100

0.747
  pilT Legionella pneumophila strain ERS1305867

74.709

100

0.747
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.517

95.64

0.674
  pilT Vibrio cholerae strain A1552

70.517

95.64

0.674
  pilT Neisseria meningitidis 8013

66.957

100

0.672
  pilT Neisseria gonorrhoeae MS11

66.667

100

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

100

0.509
  pilU Pseudomonas stutzeri DSM 10701

42.09

97.384

0.41
  pilU Vibrio cholerae strain A1552

41.846

94.477

0.395
  pilU Acinetobacter baylyi ADP1

39.017

100

0.392

Multiple sequence alignment