Detailed information NTG0116467

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: YC6258_RS03800
• Genome accession: NZ_CP007142
• Coordinates: 855468..856595 (+)
• Length: 375 a.a.
• NCBI ID: WP_044615870.1
• Uniprot ID: A0A0C5VRM7
• Organism: Gynuella sunshinyii YC6258
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 850468..861595

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
YC6258_RS03770 (YC6258_00868)	-	850638..851786 (-)	1149	WP_044615864.1	homoserine O-acetyltransferase	-
YC6258_RS03775 (YC6258_00869)	-	851801..852109 (-)	309	WP_044615865.1	DUF167 family protein	-
YC6258_RS03780 (YC6258_00870)	-	852106..852693 (-)	588	WP_044615866.1	YggT family protein	-
YC6258_RS03785 (YC6258_00871)	proC	852693..853523 (-)	831	WP_044615867.1	pyrroline-5-carboxylate reductase	-
YC6258_RS03790 (YC6258_00872)	-	853543..854232 (-)	690	WP_044615868.1	YggS family pyridoxal phosphate-dependent enzyme	-
YC6258_RS03795 (YC6258_00873)	pilT	854394..855428 (+)	1035	WP_044615869.1	type IV pilus twitching motility protein PilT	Machinery gene
YC6258_RS03800 (YC6258_00874)	pilU	855468..856595 (+)	1128	WP_044615870.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
YC6258_RS03805	-	857052..857486 (+)	435	WP_044615871.1	hypothetical protein	-
YC6258_RS03810 (YC6258_00877)	hemH	857527..858558 (-)	1032	WP_044615872.1	ferrochelatase	-
YC6258_RS03815 (YC6258_00878)	-	858652..859275 (-)	624	WP_082070547.1	LysE family translocator	-
YC6258_RS03820 (YC6258_00879)	glpE	859272..859568 (-)	297	WP_044619702.1	thiosulfate sulfurtransferase GlpE	-

Sequence

Protein

• Length: 375 a.a.
• Molecular weight: 42235.44 Da
• Isoelectric Point: 6.6929

>NTDB_id=116467 YC6258_RS03800 WP_044615870.1 855468..856595(+) (pilU) [Gynuella sunshinyii YC6258]
MEFDKLLRLMVEKGASDLFITAGVAPSMKIHGQVVPITKNPLSPEKTRELVLSVMTERQRESFIDTNECNFAISARGIGR
FRVSAFFQRNLTAMVLRRIETRIPRTEELGLPDVLKQMAMTKRGLVIFVGATGTGKSTSLAAMIGHRNRNSKGHIISIED
PIEFIHQHEGCIVTQREVGIDTESFEVALKNTLRQAPDVIMIGEVRTKETMEYAITFAETGHLCLATLHANNANQALDRI
MHFFPSERQRQLWMDLSLNLRGIVAQQLIPTPDGTGRRAVVEVLLNSPLVQDHIRKGEVHLLKDLMAKSNELGMQTFDQA
LFHLYTRGEITYQDALAHADSANDLRLMIKLDQETDSDHLDDKSSRFTIQDGDNY

Nucleotide

• Length: 1128 bp

>NTDB_id=116467 YC6258_RS03800 WP_044615870.1 855468..856595(+) (pilU) [Gynuella sunshinyii YC6258]
ATGGAATTCGATAAGTTACTGCGGCTGATGGTTGAAAAAGGCGCATCGGATCTGTTTATTACGGCTGGTGTGGCACCGAG
TATGAAAATTCATGGTCAAGTGGTGCCGATTACCAAGAACCCTCTATCACCGGAAAAAACCCGTGAACTGGTGTTGTCGG
TTATGACCGAACGGCAAAGAGAAAGTTTTATCGACACCAATGAGTGTAATTTTGCCATTTCCGCCCGGGGCATTGGTCGT
TTCCGGGTGAGTGCATTCTTTCAACGTAACCTCACAGCCATGGTGCTGAGACGCATCGAGACCCGTATTCCGCGAACAGA
AGAGCTGGGTCTGCCGGACGTATTGAAGCAGATGGCCATGACCAAGCGCGGGCTGGTGATTTTCGTCGGTGCGACCGGTA
CCGGTAAGTCCACTTCGCTGGCAGCCATGATTGGGCATCGTAACCGCAATTCCAAAGGTCATATTATTTCCATTGAGGAT
CCGATCGAATTTATCCATCAGCACGAAGGTTGTATCGTGACTCAGCGCGAGGTTGGTATCGACACAGAATCATTTGAAGT
GGCTCTGAAAAATACTCTGCGGCAGGCGCCGGACGTCATTATGATCGGTGAGGTACGAACCAAGGAAACCATGGAGTATG
CCATCACGTTTGCAGAAACCGGGCACTTGTGTCTGGCGACATTGCACGCCAACAACGCCAACCAGGCGCTGGACCGGATC
ATGCACTTTTTCCCGTCGGAACGGCAACGGCAGCTGTGGATGGACCTGTCATTGAACCTGCGTGGTATTGTGGCGCAGCA
ATTGATTCCGACCCCCGATGGAACCGGGCGCCGGGCGGTGGTCGAAGTACTGTTGAACTCGCCTTTGGTTCAGGACCATA
TTCGTAAAGGTGAGGTGCACCTGCTGAAGGATCTCATGGCTAAATCCAATGAACTGGGTATGCAGACCTTCGATCAGGCA
TTGTTCCACTTGTACACTCGTGGCGAGATTACTTATCAGGATGCTCTGGCACATGCTGACTCTGCCAATGATCTGCGTCT
GATGATCAAGCTTGATCAGGAAACCGATTCCGATCACCTGGATGACAAATCCAGTCGTTTTACCATTCAGGATGGAGACA
ACTATTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (114-269)

3D structure

• AlphaFold DB: A0A0C5VRM7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	75.401	99.733	0.752
pilU	Acinetobacter baylyi ADP1	64.146	95.2	0.611
pilU	Vibrio cholerae strain A1552	58.146	94.933	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	44.248	90.4	0.4