Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ABNT83_RS02145 Genome accession   NZ_OZ026884
Coordinates   451091..452125 (-) Length   344 a.a.
NCBI ID   WP_348758803.1    Uniprot ID   -
Organism   Candidatus Methylocalor cossyra isolate MeCH1-AG     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 446091..457125
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABNT83_RS02120 (MECH1_V1_0438) - 446688..446924 (-) 237 WP_348758798.1 exodeoxyribonuclease VII small subunit -
  ABNT83_RS02125 (MECH1_V1_0439) sseA 447052..447909 (-) 858 WP_348758799.1 3-mercaptopyruvate sulfurtransferase -
  ABNT83_RS02130 (MECH1_V1_0440) dapE 447930..449060 (-) 1131 WP_348758800.1 succinyl-diaminopimelate desuccinylase -
  ABNT83_RS02135 (MECH1_V1_0441) - 449227..449790 (-) 564 WP_348758801.1 DUF2726 domain-containing protein -
  ABNT83_RS02140 (MECH1_V1_0442) pilU 449949..451070 (-) 1122 WP_348758802.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABNT83_RS02145 (MECH1_V1_0443) pilT 451091..452125 (-) 1035 WP_348758803.1 type IV pilus twitching motility protein PilT Machinery gene
  ABNT83_RS02150 (MECH1_V1_0444) - 452217..452951 (+) 735 WP_348758804.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABNT83_RS02155 (MECH1_V1_0445) proC 452983..453807 (+) 825 WP_348758805.1 pyrroline-5-carboxylate reductase -
  ABNT83_RS02160 (MECH1_V1_0446) - 453810..454385 (+) 576 WP_348758806.1 YggT family protein -
  ABNT83_RS02165 (MECH1_V1_0447) - 454385..454687 (+) 303 WP_348758807.1 DUF167 family protein -
  ABNT83_RS02170 (MECH1_V1_0448) - 454765..456753 (+) 1989 WP_348758808.1 dynamin family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38240.94 Da        Isoelectric Point: 7.0364

>NTDB_id=1163285 ABNT83_RS02145 WP_348758803.1 451091..452125(-) (pilT) [Candidatus Methylocalor cossyra isolate MeCH1-AG]
MDIAELLAFTAKNNASDLHLSAGLPPMIRVDGDIRRINVPPLDHKEVHALVYDIMNDKQRRDYEEFLETDFSFELPGVAR
FRVNAFNQQRGAAAVFRTIPSKVLTLEELGCPRFFQEVVRQPRGLILVTGPTGSGKSTTLAAMIEHINANDYLHILTIED
PIEFVHTSKKSLINQREVHRDTLGFNEALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFATLHTNSAAKTIDRI
VDVFPAAEKGIIRSMLSESLQAVISQTLVKKNGGGRVAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGRKDGMQTMDQHL
QELVEKGIISRQAARAKAVNKAAF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1163285 ABNT83_RS02145 WP_348758803.1 451091..452125(-) (pilT) [Candidatus Methylocalor cossyra isolate MeCH1-AG]
ATGGACATCGCTGAGTTGCTCGCCTTCACCGCCAAGAACAACGCTTCCGACCTGCATCTGTCGGCGGGGCTCCCGCCCAT
GATCCGGGTGGATGGCGACATCCGCCGCATCAACGTGCCTCCCCTCGACCACAAGGAGGTGCACGCCCTGGTGTACGACA
TCATGAACGATAAGCAGCGCCGGGACTACGAGGAGTTCCTGGAAACCGACTTCTCGTTCGAGTTGCCGGGGGTGGCGCGC
TTCCGCGTCAATGCGTTCAACCAGCAGCGTGGGGCCGCGGCGGTGTTCCGGACCATCCCGTCCAAGGTCCTGACCCTCGA
GGAGTTGGGGTGTCCCCGGTTCTTCCAGGAGGTGGTGCGGCAGCCGCGTGGCCTGATCCTGGTCACCGGCCCCACCGGTT
CCGGGAAATCCACCACCCTGGCGGCCATGATCGAACACATCAACGCCAACGACTACCTGCACATCCTCACCATCGAGGAT
CCCATCGAGTTCGTCCATACCAGCAAGAAATCCCTCATCAACCAGCGCGAAGTGCACCGCGACACCCTGGGCTTCAACGA
GGCCTTGCGGTCCGCCTTGCGCGAGGACCCGGATGTGATCCTGGTGGGGGAGATGCGCGACCTGGAGACCATACGCCTGG
CCCTAACCGCAGCCGAGACCGGTCACCTGGTATTCGCCACCTTGCACACCAACTCGGCGGCCAAGACCATCGACCGCATC
GTGGATGTGTTTCCAGCGGCGGAGAAGGGCATCATCCGTTCCATGCTGTCCGAATCCCTGCAGGCAGTCATTTCCCAGAC
GCTGGTGAAAAAGAACGGCGGCGGGCGGGTGGCGGCCTGGGAAATCATGGTGGGCACCCCGGCGATCCGCAATCTGATCC
GCGAGGACAAGGTCGCGCAAATGTATTCGGCGATCCAGACCGGCCGCAAGGACGGGATGCAGACCATGGACCAGCATCTG
CAGGAGCTGGTGGAGAAAGGGATCATTTCCCGCCAGGCGGCGCGGGCCAAGGCCGTCAACAAGGCGGCGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

81.361

98.256

0.799
  pilT Pseudomonas stutzeri DSM 10701

78.488

100

0.785
  pilT Acinetobacter nosocomialis M2

77.907

100

0.779
  pilT Acinetobacter baumannii D1279779

77.907

100

0.779
  pilT Acinetobacter baumannii strain A118

77.907

100

0.779
  pilT Acinetobacter baylyi ADP1

76.744

100

0.767
  pilT Legionella pneumophila strain Lp02

73.547

100

0.735
  pilT Legionella pneumophila strain ERS1305867

73.547

100

0.735
  pilT Neisseria gonorrhoeae MS11

68.116

100

0.683
  pilT Neisseria meningitidis 8013

68.116

100

0.683
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.953

97.965

0.666
  pilT Vibrio cholerae strain A1552

67.953

97.965

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.754

100

0.529
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.384

0.398
  pilU Acinetobacter baylyi ADP1

41.265

96.512

0.398
  pilU Vibrio cholerae strain A1552

40.597

97.384

0.395

Multiple sequence alignment