Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABNT83_RS02140 Genome accession   NZ_OZ026884
Coordinates   449949..451070 (-) Length   373 a.a.
NCBI ID   WP_348758802.1    Uniprot ID   -
Organism   Candidatus Methylocalor cossyra isolate MeCH1-AG     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 444949..456070
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABNT83_RS02115 (MECH1_V1_0437) ispA 445802..446698 (-) 897 WP_348758797.1 (2E,6E)-farnesyl diphosphate synthase -
  ABNT83_RS02120 (MECH1_V1_0438) - 446688..446924 (-) 237 WP_348758798.1 exodeoxyribonuclease VII small subunit -
  ABNT83_RS02125 (MECH1_V1_0439) sseA 447052..447909 (-) 858 WP_348758799.1 3-mercaptopyruvate sulfurtransferase -
  ABNT83_RS02130 (MECH1_V1_0440) dapE 447930..449060 (-) 1131 WP_348758800.1 succinyl-diaminopimelate desuccinylase -
  ABNT83_RS02135 (MECH1_V1_0441) - 449227..449790 (-) 564 WP_348758801.1 DUF2726 domain-containing protein -
  ABNT83_RS02140 (MECH1_V1_0442) pilU 449949..451070 (-) 1122 WP_348758802.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABNT83_RS02145 (MECH1_V1_0443) pilT 451091..452125 (-) 1035 WP_348758803.1 type IV pilus twitching motility protein PilT Machinery gene
  ABNT83_RS02150 (MECH1_V1_0444) - 452217..452951 (+) 735 WP_348758804.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABNT83_RS02155 (MECH1_V1_0445) proC 452983..453807 (+) 825 WP_348758805.1 pyrroline-5-carboxylate reductase -
  ABNT83_RS02160 (MECH1_V1_0446) - 453810..454385 (+) 576 WP_348758806.1 YggT family protein -
  ABNT83_RS02165 (MECH1_V1_0447) - 454385..454687 (+) 303 WP_348758807.1 DUF167 family protein -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41961.39 Da        Isoelectric Point: 6.7742

>NTDB_id=1163284 ABNT83_RS02140 WP_348758802.1 449949..451070(-) (pilU) [Candidatus Methylocalor cossyra isolate MeCH1-AG]
MEFESLLMLMAHKKASDLFITANKEPCVKIDGQLQPLSATKLTKEQAYQLVVSAMNQRQRDEFENTKECNFALSVEGLGR
FRVSAFVQRDAAGMVLRRIESRIPSAEELHLPPILNELAMTKRGLILFVGATGTGKSSSLAALIKYRNEHSTGHIITIED
PLEFIHPHAGCIVTQREVGIDTESYEVALKNTLRQAPDVILIGEIRTRETMQYALAFAETGHLCLSTLHANNANQALDRI
LHFFPEDMRPQVFMDLSLNLKAIIAQQLVRRADGKGRYPALEILINTPLASDLIRKGEIHKLKDLMKRSREHGMLTFDQC
LYELYKAGKISYEDALHAADSRNEVRLMIKLSEEGSIDKYAPPSDKVFIVDDT

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=1163284 ABNT83_RS02140 WP_348758802.1 449949..451070(-) (pilU) [Candidatus Methylocalor cossyra isolate MeCH1-AG]
GTGGAGTTCGAGTCTTTGCTCATGCTGATGGCGCACAAAAAGGCGTCGGATCTCTTCATCACCGCCAACAAGGAGCCCTG
CGTCAAAATCGACGGCCAACTCCAGCCCCTTTCCGCCACCAAGCTGACCAAGGAGCAAGCCTACCAGCTGGTGGTCAGCG
CCATGAACCAACGCCAGCGGGACGAATTCGAGAACACCAAGGAGTGCAATTTCGCCCTCTCCGTCGAGGGCTTGGGGCGC
TTCCGGGTGAGCGCCTTCGTGCAGCGCGACGCCGCCGGCATGGTGTTGCGCCGGATCGAGAGCCGGATCCCCTCGGCCGA
GGAGCTCCACCTGCCGCCCATCCTCAACGAGCTGGCCATGACCAAGCGCGGCCTGATTCTATTCGTGGGCGCCACCGGTA
CCGGCAAATCCAGCTCCCTGGCGGCTTTGATCAAATATCGCAACGAGCACTCCACGGGCCATATCATCACCATCGAGGAC
CCTTTGGAGTTCATCCATCCCCATGCCGGCTGCATCGTCACCCAGCGGGAAGTGGGCATCGACACGGAATCCTATGAGGT
GGCCCTGAAGAACACCCTCCGCCAGGCGCCGGACGTGATCCTGATCGGCGAAATTCGCACCCGCGAGACCATGCAGTACG
CCCTGGCCTTCGCCGAGACCGGGCATCTGTGCCTTTCCACCCTGCATGCCAACAACGCCAACCAGGCGCTGGATAGAATC
CTGCACTTCTTCCCCGAGGACATGCGCCCGCAGGTGTTCATGGACCTGTCCCTCAATCTCAAGGCCATCATTGCCCAGCA
GCTGGTCCGCCGCGCGGACGGCAAGGGGCGCTATCCGGCGTTGGAGATCCTGATCAACACGCCCTTGGCCTCCGACCTGA
TCCGCAAGGGCGAAATCCACAAGCTGAAGGATCTGATGAAACGCTCCCGCGAGCACGGCATGCTGACCTTCGACCAATGC
CTCTACGAGCTTTACAAGGCCGGCAAGATCAGCTACGAGGACGCCCTGCACGCCGCCGACTCCAGGAACGAAGTGCGGCT
GATGATCAAGCTGAGCGAGGAGGGCAGCATCGACAAATACGCCCCGCCGAGCGACAAGGTGTTCATCGTTGATGACACCT
AG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.362

94.906

0.649
  pilU Acinetobacter baylyi ADP1

66.947

95.71

0.641
  pilU Vibrio cholerae strain A1552

58.857

93.834

0.552
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.327

98.391

0.397
  pilT Legionella pneumophila strain Lp02

41.888

90.885

0.381
  pilT Legionella pneumophila strain ERS1305867

41.888

90.885

0.381
  pilT Pseudomonas aeruginosa PAK

39.823

90.885

0.362

Multiple sequence alignment