Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   U9J37_RS13435 Genome accession   NZ_OY808997
Coordinates   2888100..2889140 (+) Length   346 a.a.
NCBI ID   WP_005475129.1    Uniprot ID   A0A0A5HS50
Organism   Vibrio sp. 16 isolate VDT1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2883100..2894140
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U9J37_RS13400 (VDT1_2730) hemW 2883286..2884461 (-) 1176 WP_005475165.1 radical SAM family heme chaperone HemW -
  U9J37_RS13405 (VDT1_2731) - 2884467..2885066 (-) 600 WP_005475148.1 XTP/dITP diphosphatase -
  U9J37_RS13410 (VDT1_2732) - 2885170..2885601 (-) 432 WP_005475132.1 DUF4426 domain-containing protein -
  U9J37_RS13415 (VDT1_2733) yggU 2885633..2885923 (-) 291 WP_005475152.1 DUF167 family protein YggU -
  U9J37_RS13420 (VDT1_2734) - 2885923..2886480 (-) 558 WP_005475177.1 YggT family protein -
  U9J37_RS13425 (VDT1_2735) proC 2886518..2887336 (-) 819 WP_005475241.1 pyrroline-5-carboxylate reductase -
  U9J37_RS13430 (VDT1_2736) - 2887361..2888074 (-) 714 WP_005475219.1 YggS family pyridoxal phosphate-dependent enzyme -
  U9J37_RS13435 (VDT1_2737) pilT 2888100..2889140 (+) 1041 WP_005475129.1 type IV pilus twitching motility protein PilT Machinery gene
  U9J37_RS13440 (VDT1_2738) pilU 2889152..2890258 (+) 1107 WP_043887287.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  U9J37_RS13445 (VDT1_2739) - 2890297..2890455 (-) 159 WP_005475144.1 FeoB-associated Cys-rich membrane protein -
  U9J37_RS13450 (VDT1_2740) feoB 2890457..2892958 (-) 2502 WP_005475223.1 ferrous iron transport protein B -
  U9J37_RS13455 (VDT1_2741) - 2892969..2893208 (-) 240 WP_005475125.1 FeoA family protein -
  U9J37_RS13460 (VDT1_2742) - 2893422..2893733 (+) 312 WP_043887285.1 hypothetical protein -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38377.69 Da        Isoelectric Point: 6.2286

>NTDB_id=1161993 U9J37_RS13435 WP_005475129.1 2888100..2889140(+) (pilT) [Vibrio sp. 16 isolate VDT1]
MDIAELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLGVPAFSHSDVHRLIFEIMNDAQRSEFEERLEVDFSFELPNVGR
FRVNAFNQSRGSSAVFRTIPTDIPTLEQLEAPQIFEKIANMEKGLVLVTGPTGSGKSTTLAAMVDHINRHQNKHILTIED
PIEFVHTNNKCLINQREVHRDTHSFSNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRAVIAQKLLKRIGGGRTACHEIMMATPAIRNLVREDKVAQMYSVIQTGAAHGMQTMEQHA
KQLIAQGQVDPDEVAKKIETESNSLF

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=1161993 U9J37_RS13435 WP_005475129.1 2888100..2889140(+) (pilT) [Vibrio sp. 16 isolate VDT1]
ATGGATATCGCTGAATTACTGGATTTTAGTGTAAAGCATAATGCATCAGATCTACATCTTTCTGCGGGTGTTTCACCCAT
GGTACGTATAGATGGAGAAGTAAGAAAGCTTGGTGTTCCTGCTTTTAGTCATTCTGATGTGCACCGTTTGATTTTTGAGA
TCATGAACGATGCTCAGCGAAGTGAGTTTGAAGAGCGGCTTGAAGTCGATTTTTCATTTGAGTTACCTAACGTTGGTCGA
TTCCGTGTCAACGCGTTCAACCAGTCTCGCGGTAGCTCTGCGGTGTTTCGTACCATTCCAACCGATATTCCAACCTTAGA
GCAATTGGAAGCGCCGCAAATTTTCGAAAAGATCGCCAATATGGAGAAGGGCTTGGTTTTGGTGACCGGACCAACCGGCT
CGGGTAAGTCGACAACGCTTGCCGCCATGGTGGATCACATTAACCGCCACCAGAATAAACACATCCTTACCATCGAAGAT
CCTATCGAATTTGTTCACACCAACAACAAGTGCCTGATAAACCAACGCGAAGTGCACCGCGATACCCACAGCTTTAGTAA
TGCGCTGCGTAGTGCGTTGCGTGAAGATCCCGATGTCATTTTGGTGGGTGAGCTGCGTGACCAAGAGACGATCAGCTTGG
CGCTGACGGCGGCGGAAACAGGCCATTTAGTGTTTGGTACGCTGCATACCAGTAGTGCCGCTAAGACCATTGACCGTATT
ATCGATGTTTTCCCTGGTAGCGATAAAGACATGGTGCGATCCATGTTGTCGGAGTCACTGCGTGCTGTTATCGCGCAGAA
ACTGCTGAAGCGGATTGGTGGAGGGCGCACCGCCTGCCATGAGATCATGATGGCGACCCCAGCGATTCGAAACTTGGTTC
GTGAAGACAAAGTGGCACAGATGTATTCAGTGATCCAAACTGGCGCAGCGCACGGCATGCAGACTATGGAGCAGCACGCC
AAGCAGTTGATTGCTCAAGGTCAGGTGGATCCTGATGAAGTGGCGAAGAAGATCGAAACTGAATCCAATAGTTTGTTCTG
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0A5HS50

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

90.029

98.555

0.887
  pilT Vibrio cholerae strain A1552

90.029

98.555

0.887
  pilT Acinetobacter baumannii D1279779

71.299

95.665

0.682
  pilT Acinetobacter baumannii strain A118

71.299

95.665

0.682
  pilT Acinetobacter nosocomialis M2

70.997

95.665

0.679
  pilT Acinetobacter baylyi ADP1

71.212

95.376

0.679
  pilT Pseudomonas stutzeri DSM 10701

67.638

99.133

0.671
  pilT Pseudomonas aeruginosa PAK

66.472

99.133

0.659
  pilT Legionella pneumophila strain Lp02

68.827

93.642

0.645
  pilT Legionella pneumophila strain ERS1305867

68.827

93.642

0.645
  pilT Neisseria meningitidis 8013

63.873

100

0.639
  pilT Neisseria gonorrhoeae MS11

62.248

100

0.624
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.719

100

0.512
  pilU Vibrio cholerae strain A1552

41.818

95.376

0.399
  pilU Pseudomonas stutzeri DSM 10701

40.606

95.376

0.387
  pilU Acinetobacter baylyi ADP1

40.938

92.486

0.379

Multiple sequence alignment