Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   U9J37_RS13440 Genome accession   NZ_OY808997
Coordinates   2889152..2890258 (+) Length   368 a.a.
NCBI ID   WP_043887287.1    Uniprot ID   -
Organism   Vibrio sp. 16 isolate VDT1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2884152..2895258
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U9J37_RS13405 (VDT1_2731) - 2884467..2885066 (-) 600 WP_005475148.1 XTP/dITP diphosphatase -
  U9J37_RS13410 (VDT1_2732) - 2885170..2885601 (-) 432 WP_005475132.1 DUF4426 domain-containing protein -
  U9J37_RS13415 (VDT1_2733) yggU 2885633..2885923 (-) 291 WP_005475152.1 DUF167 family protein YggU -
  U9J37_RS13420 (VDT1_2734) - 2885923..2886480 (-) 558 WP_005475177.1 YggT family protein -
  U9J37_RS13425 (VDT1_2735) proC 2886518..2887336 (-) 819 WP_005475241.1 pyrroline-5-carboxylate reductase -
  U9J37_RS13430 (VDT1_2736) - 2887361..2888074 (-) 714 WP_005475219.1 YggS family pyridoxal phosphate-dependent enzyme -
  U9J37_RS13435 (VDT1_2737) pilT 2888100..2889140 (+) 1041 WP_005475129.1 type IV pilus twitching motility protein PilT Machinery gene
  U9J37_RS13440 (VDT1_2738) pilU 2889152..2890258 (+) 1107 WP_043887287.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  U9J37_RS13445 (VDT1_2739) - 2890297..2890455 (-) 159 WP_005475144.1 FeoB-associated Cys-rich membrane protein -
  U9J37_RS13450 (VDT1_2740) feoB 2890457..2892958 (-) 2502 WP_005475223.1 ferrous iron transport protein B -
  U9J37_RS13455 (VDT1_2741) - 2892969..2893208 (-) 240 WP_005475125.1 FeoA family protein -
  U9J37_RS13460 (VDT1_2742) - 2893422..2893733 (+) 312 WP_043887285.1 hypothetical protein -
  U9J37_RS13465 (VDT1_2743) - 2893757..2894542 (+) 786 WP_005475221.1 DUF4198 domain-containing protein -
  U9J37_RS13470 (VDT1_2744) ruvX 2894613..2895038 (-) 426 WP_038137825.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41443.36 Da        Isoelectric Point: 7.1146

>NTDB_id=1161994 U9J37_RS13440 WP_043887287.1 2889152..2890258(+) (pilU) [Vibrio sp. 16 isolate VDT1]
MDINAYLQGMNNQKSSDLYITVGAPILYRVDGELRAHGEKLSLNDVDILLRSMMDQDRQREFHSSKEANFAIVRDFGRYR
VSAFYQRELPGAVIRRIETTIPSFADLRLPDVLQDLSIAKRGLVLVVGATGSGKSTTMAAMTGYRNTHRTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLRGVIGQQLIRDKNGQGRHGVFEILLNSPRVSDLIRRGELHELKSTMAKSKEIGMQTFDQALY
QLVIDGKINEKDALHSADSANDLRIMLKTRRGDGFAGGSLSNVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=1161994 U9J37_RS13440 WP_043887287.1 2889152..2890258(+) (pilU) [Vibrio sp. 16 isolate VDT1]
ATGGATATTAATGCTTACCTTCAGGGAATGAACAACCAAAAATCGTCTGATCTATACATTACCGTAGGCGCTCCGATCCT
TTATCGAGTAGACGGGGAACTGCGTGCGCATGGTGAGAAGCTGTCACTGAATGATGTGGATATCCTACTCAGAAGTATGA
TGGATCAAGATCGGCAGCGAGAGTTCCATTCAAGCAAAGAGGCGAACTTTGCCATAGTACGAGACTTTGGTCGCTATCGA
GTATCGGCGTTTTACCAACGAGAGCTACCTGGCGCGGTGATTCGCCGCATTGAAACCACGATTCCTAGCTTTGCAGATTT
ACGCTTGCCTGATGTGCTGCAAGATTTGTCGATCGCCAAGCGAGGGCTCGTGTTGGTCGTCGGTGCTACAGGGTCGGGGA
AATCCACCACCATGGCGGCAATGACGGGCTATCGAAATACCCACCGAACTGGTCATATTCTTACCGTGGAAGATCCGATC
GAGTTTGTGCATGAGCATAAGCGTTGTATCGTCACTCAGCGTGAGGTGGGGCTAGATACTGAAAGCTATGAAGTCGCGCT
TAAAAACTCGCTGCGCCAAGCGCCGGATATGATCTTAATTGGCGAAATTCGTTCTCGTGAAACCATGGAATACGCGATGA
CGTTTGCTGAAACGGGTCACCTGTGTATGGCGACGCTCCACGCGAACAACGCAAACCAAGCGCTTGAGCGTATCTTGCAT
TTGGTGCCTAAAGAGCAAAAAGAGCAGTTCTTGTTTGATTTGTCGATGAACCTACGCGGCGTGATAGGTCAACAGTTGAT
TCGTGATAAGAATGGTCAAGGGCGTCACGGTGTATTTGAAATTCTGCTTAATAGCCCACGCGTGTCAGACCTGATTCGCC
GAGGAGAGCTGCATGAGTTGAAATCGACCATGGCTAAATCAAAAGAGATAGGCATGCAAACCTTTGACCAAGCTTTATAT
CAGTTGGTTATCGATGGAAAAATCAATGAAAAAGATGCCTTGCACAGTGCGGACTCCGCGAATGACTTGCGCATTATGCT
AAAGACAAGGCGAGGTGACGGCTTCGCTGGTGGCTCTTTGAGTAATGTGAAAATTGATATGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

83.152

100

0.832
  pilU Pseudomonas stutzeri DSM 10701

56.286

95.109

0.535
  pilU Acinetobacter baylyi ADP1

53.846

95.38

0.514
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.37

88.043

0.399
  pilT Legionella pneumophila strain Lp02

42.773

92.12

0.394
  pilT Legionella pneumophila strain ERS1305867

42.773

92.12

0.394
  pilT Acinetobacter baylyi ADP1

41.642

92.663

0.386
  pilT Acinetobacter baumannii strain A118

41.298

92.12

0.38
  pilT Acinetobacter nosocomialis M2

41.298

92.12

0.38
  pilT Acinetobacter baumannii D1279779

41.298

92.12

0.38
  pilT Pseudomonas aeruginosa PAK

41.003

92.12

0.378
  pilT Pseudomonas stutzeri DSM 10701

40.118

92.12

0.37
  pilT Vibrio cholerae strain A1552

40.12

90.761

0.364
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.12

90.761

0.364

Multiple sequence alignment