Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACLMBM_RS08490 Genome accession   NZ_OY781972
Coordinates   1940922..1941959 (-) Length   345 a.a.
NCBI ID   WP_321276082.1    Uniprot ID   -
Organism   MAG: Thiomicrorhabdus indica isolate 830269dd-2df0-462d-a2dc-71ec7b730e8f     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1935922..1946959
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLMBM_RS08475 (U2908_RS08460) - 1937455..1938021 (+) 567 WP_321276079.1 tetratricopeptide repeat protein -
  ACLMBM_RS08480 (U2908_RS08465) - 1938030..1939223 (+) 1194 WP_321276080.1 murein L,D-transpeptidase family protein -
  ACLMBM_RS08485 (U2908_RS08470) pilU 1939693..1940865 (-) 1173 WP_321276081.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACLMBM_RS08490 (U2908_RS08475) pilT 1940922..1941959 (-) 1038 WP_321276082.1 type IV pilus twitching motility protein PilT Machinery gene
  ACLMBM_RS08495 (U2908_RS08480) - 1942191..1942649 (-) 459 WP_321276083.1 DNA polymerase III subunit chi -
  ACLMBM_RS08500 (U2908_RS08485) - 1942733..1944211 (-) 1479 WP_321276084.1 leucyl aminopeptidase -
  ACLMBM_RS08505 (U2908_RS08490) lptF 1944491..1945645 (+) 1155 WP_321276085.1 LPS export ABC transporter permease LptF -
  ACLMBM_RS08510 (U2908_RS08495) lptG 1945642..1946733 (+) 1092 WP_321276086.1 LPS export ABC transporter permease LptG -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38341.71 Da        Isoelectric Point: 5.1351

>NTDB_id=1161880 ACLMBM_RS08490 WP_321276082.1 1940922..1941959(-) (pilT) [MAG: Thiomicrorhabdus indica isolate 830269dd-2df0-462d-a2dc-71ec7b730e8f]
MDITQLLEFGVQQGASDLHLSSEQPPILRINGDIQRIDAPAIDGEELQKMIYDVMSDNQRRDFETNLEADFSFELPNIAR
FRVNVFMQNRGIGGVFRTIPSNVLTLEQLNAPEIFKDISSLPRGIVLVTGPTGSGKSTTLAAMIDYINDNFSSHILTIED
PIEFVHTPKRALINQREVHRDTKSFENSLRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
IDVFPAEEKEMVRSMLSESIKAVISQTLLKKSTGGRVAAHEIMLSNNAIRNLIREAKIAQMYSVIQTANAQGMQTLDQNL
LELVQDGTITAEAAKRKAVNKNLFN

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1161880 ACLMBM_RS08490 WP_321276082.1 1940922..1941959(-) (pilT) [MAG: Thiomicrorhabdus indica isolate 830269dd-2df0-462d-a2dc-71ec7b730e8f]
ATGGATATTACTCAATTATTGGAGTTTGGCGTTCAACAAGGCGCCTCAGATTTACACCTTTCCAGTGAGCAACCCCCTAT
TTTAAGAATTAACGGTGATATCCAACGGATTGATGCACCTGCCATCGATGGCGAAGAGCTACAAAAAATGATCTACGATG
TCATGAGTGACAATCAACGACGCGACTTCGAAACCAATTTAGAGGCTGACTTCTCATTCGAATTGCCAAATATTGCGCGT
TTTCGTGTCAATGTCTTTATGCAGAACCGAGGAATTGGCGGAGTTTTTCGTACCATCCCAAGCAACGTTCTCACCTTAGA
ACAACTGAATGCCCCTGAAATATTTAAAGACATCTCTAGTCTGCCACGAGGTATTGTCCTAGTGACCGGCCCTACAGGTT
CGGGTAAATCCACGACTCTGGCAGCAATGATTGACTACATCAATGATAATTTCTCCTCTCACATCTTAACCATTGAAGAC
CCGATTGAGTTCGTTCATACCCCCAAACGCGCCTTGATTAACCAACGTGAAGTTCACAGGGACACTAAAAGTTTTGAAAA
CTCACTACGCTCAGCCCTTCGTGAAGACCCAGATGTGATTCTTGTCGGGGAAATGCGAGACCTTGAAACGATTCGCCTAG
CCTTAACTGCAGCCGAAACGGGGCATTTAGTCTTTGGAACCTTACACACCTCATCAGCAGCAAAAACCATTGACCGTGTT
ATCGATGTATTCCCTGCTGAAGAAAAAGAGATGGTTCGTTCAATGCTCTCTGAATCAATTAAGGCCGTTATTTCACAAAC
ACTTCTAAAGAAGTCAACCGGAGGGCGTGTCGCTGCACATGAAATCATGCTGTCGAACAATGCGATCCGTAACTTAATTC
GTGAAGCCAAAATAGCCCAAATGTATTCAGTCATACAAACCGCTAATGCTCAAGGAATGCAAACGCTTGACCAAAACCTT
TTAGAATTGGTTCAAGATGGAACCATCACTGCCGAAGCAGCGAAACGAAAAGCCGTCAACAAAAACCTATTCAACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

70.349

99.71

0.701
  pilT Acinetobacter nosocomialis M2

70.058

99.71

0.699
  pilT Acinetobacter baumannii D1279779

70.058

99.71

0.699
  pilT Acinetobacter baumannii strain A118

70.058

99.71

0.699
  pilT Pseudomonas stutzeri DSM 10701

70.71

97.971

0.693
  pilT Pseudomonas aeruginosa PAK

69.822

97.971

0.684
  pilT Neisseria gonorrhoeae MS11

66.862

98.841

0.661
  pilT Neisseria meningitidis 8013

66.862

98.841

0.661
  pilT Legionella pneumophila strain Lp02

65.116

99.71

0.649
  pilT Legionella pneumophila strain ERS1305867

65.116

99.71

0.649
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

65.282

97.681

0.638
  pilT Vibrio cholerae strain A1552

65.282

97.681

0.638
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

100

0.51
  pilU Pseudomonas stutzeri DSM 10701

40.525

99.42

0.403
  pilU Vibrio cholerae strain A1552

38.776

99.42

0.386
  pilU Acinetobacter baylyi ADP1

38.372

99.71

0.383

Multiple sequence alignment