Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACKPCU_RS03630 Genome accession   NZ_OY781932
Coordinates   791738..792772 (+) Length   344 a.a.
NCBI ID   WP_104736524.1    Uniprot ID   -
Organism   MAG: Halopseudomonas oceani isolate ab883223-6a80-4053-bf73-fb395571e785     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 786738..797772
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKPCU_RS03595 (U2971_RS03595) - 786914..787345 (-) 432 WP_321349974.1 DUF4426 domain-containing protein -
  ACKPCU_RS03600 (U2971_RS03600) metW 787368..787958 (-) 591 WP_321351729.1 methionine biosynthesis protein MetW -
  ACKPCU_RS03605 (U2971_RS03605) - 787961..789103 (-) 1143 WP_104736529.1 homoserine O-acetyltransferase -
  ACKPCU_RS03610 (U2971_RS03610) - 789228..789518 (-) 291 WP_104736528.1 DUF167 family protein -
  ACKPCU_RS03615 (U2971_RS03615) - 789527..790117 (-) 591 WP_321349978.1 YggT family protein -
  ACKPCU_RS03620 (U2971_RS03620) proC 790131..790949 (-) 819 WP_321349980.1 pyrroline-5-carboxylate reductase -
  ACKPCU_RS03625 (U2971_RS03625) - 790974..791660 (-) 687 WP_321349981.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACKPCU_RS03630 (U2971_RS03630) pilT 791738..792772 (+) 1035 WP_104736524.1 type IV pilus twitching motility protein PilT Machinery gene
  ACKPCU_RS03635 (U2971_RS03635) pilU 792834..793988 (+) 1155 WP_321349984.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACKPCU_RS03640 (U2971_RS03640) siaD 794009..794821 (-) 813 WP_104736522.1 biofilm regulation diguanylate cyclase SiaD -
  ACKPCU_RS03645 (U2971_RS03645) siaC 794829..795206 (-) 378 WP_104736521.1 biofilm regulation phosphoprotein SiaC -
  ACKPCU_RS03650 (U2971_RS03650) siaB 795229..795771 (-) 543 WP_104736520.1 biofilm regulation protein kinase SiaB -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37776.61 Da        Isoelectric Point: 6.8359

>NTDB_id=1161843 ACKPCU_RS03630 WP_104736524.1 791738..792772(+) (pilT) [MAG: Halopseudomonas oceani isolate ab883223-6a80-4053-bf73-fb395571e785]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINVPAMEHKQVHALIYDIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGDVFKKISDVPRGLVLVTGPTGSGKSTSLAAMLDYLNSTKYHHILTIED
PIEFVHESKKCLVNQREVHRDTLGFAEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
VDVFPGEEKAMVRSMLSESLQAVVSQTLLKKIGGGRVAAHEIMIGTPAIRNLIREDKIAQMYSAIQTGGNLGMQTLDMCL
KSLVAKGLVSKESAREKAKVADGF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1161843 ACKPCU_RS03630 WP_104736524.1 791738..792772(+) (pilT) [MAG: Halopseudomonas oceani isolate ab883223-6a80-4053-bf73-fb395571e785]
ATGGATATTACCGAGCTGTTAGCCTTCAGCGCTAAACAGGGCGCGTCGGACTTGCACCTGTCGGCAGGACTGCCGCCCAT
GATCCGTGTTGACGGCGACGTGCGCCGGATCAACGTACCGGCGATGGAGCACAAGCAGGTTCATGCGCTGATCTACGACA
TCATGAATGACAAGCAGCGCAAGGACTATGAGGAGTTTCTGGAAACCGACTTCTCGTTCGAGGTTCCCGGTGTGGCCCGC
TTCCGGGTCAATGCGTTCAACCAGAATCGTGGCGCAGGCGCCGTATTCCGGACCATTCCTTCCAAGGTACTGACCATGGA
AGATCTGGGCATGGGCGATGTGTTCAAGAAAATTTCCGATGTGCCGCGCGGTCTGGTGCTGGTCACCGGCCCGACCGGTT
CGGGCAAGTCGACCAGTCTCGCGGCCATGCTCGACTATCTCAACTCCACCAAGTATCACCACATCCTGACCATTGAAGAC
CCGATCGAATTTGTTCACGAGTCGAAGAAGTGTCTGGTCAACCAGCGTGAAGTACACCGTGACACACTTGGCTTTGCCGA
AGCGTTGCGCTCGGCACTGCGGGAAGACCCGGATATTATCCTGGTTGGTGAGATGCGTGACTTGGAAACCATCCGTTTGG
CGCTGACTGCGGCAGAAACCGGTCACCTGGTGTTCGGCACCCTGCACACCACGTCGGCCGCGAAGACCATTGACCGTATC
GTCGACGTGTTCCCCGGTGAAGAAAAAGCGATGGTGCGCTCGATGCTGTCCGAGTCACTACAGGCGGTTGTCTCTCAGAC
CCTGCTGAAGAAGATCGGCGGCGGTCGGGTGGCTGCCCACGAAATCATGATCGGTACCCCGGCGATCCGTAACCTGATCC
GTGAAGACAAGATCGCGCAGATGTACTCGGCGATCCAGACCGGTGGCAACCTCGGCATGCAGACCCTCGACATGTGTCTG
AAGTCGTTGGTCGCCAAGGGGCTGGTCAGCAAGGAAAGCGCGCGTGAAAAGGCCAAGGTCGCTGACGGGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

92.442

100

0.924
  pilT Pseudomonas stutzeri DSM 10701

91.57

100

0.916
  pilT Acinetobacter baumannii D1279779

83.14

100

0.831
  pilT Acinetobacter baumannii strain A118

83.14

100

0.831
  pilT Acinetobacter nosocomialis M2

82.849

100

0.828
  pilT Acinetobacter baylyi ADP1

80.814

100

0.808
  pilT Legionella pneumophila strain Lp02

75.444

98.256

0.741
  pilT Legionella pneumophila strain ERS1305867

75.444

98.256

0.741
  pilT Neisseria meningitidis 8013

67.536

100

0.677
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.139

97.965

0.677
  pilT Vibrio cholerae strain A1552

69.139

97.965

0.677
  pilT Neisseria gonorrhoeae MS11

67.246

100

0.674
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.917

98.547

0.512
  pilU Pseudomonas stutzeri DSM 10701

41.791

97.384

0.407
  pilU Vibrio cholerae strain A1552

39.942

99.709

0.398
  pilU Acinetobacter baylyi ADP1

39.118

98.837

0.387

Multiple sequence alignment