Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACKPCU_RS03635 Genome accession   NZ_OY781932
Coordinates   792834..793988 (+) Length   384 a.a.
NCBI ID   WP_321349984.1    Uniprot ID   -
Organism   MAG: Halopseudomonas oceani isolate ab883223-6a80-4053-bf73-fb395571e785     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 787834..798988
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKPCU_RS03605 (U2971_RS03605) - 787961..789103 (-) 1143 WP_104736529.1 homoserine O-acetyltransferase -
  ACKPCU_RS03610 (U2971_RS03610) - 789228..789518 (-) 291 WP_104736528.1 DUF167 family protein -
  ACKPCU_RS03615 (U2971_RS03615) - 789527..790117 (-) 591 WP_321349978.1 YggT family protein -
  ACKPCU_RS03620 (U2971_RS03620) proC 790131..790949 (-) 819 WP_321349980.1 pyrroline-5-carboxylate reductase -
  ACKPCU_RS03625 (U2971_RS03625) - 790974..791660 (-) 687 WP_321349981.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACKPCU_RS03630 (U2971_RS03630) pilT 791738..792772 (+) 1035 WP_104736524.1 type IV pilus twitching motility protein PilT Machinery gene
  ACKPCU_RS03635 (U2971_RS03635) pilU 792834..793988 (+) 1155 WP_321349984.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACKPCU_RS03640 (U2971_RS03640) siaD 794009..794821 (-) 813 WP_104736522.1 biofilm regulation diguanylate cyclase SiaD -
  ACKPCU_RS03645 (U2971_RS03645) siaC 794829..795206 (-) 378 WP_104736521.1 biofilm regulation phosphoprotein SiaC -
  ACKPCU_RS03650 (U2971_RS03650) siaB 795229..795771 (-) 543 WP_104736520.1 biofilm regulation protein kinase SiaB -
  ACKPCU_RS03655 (U2971_RS03655) siaA 795800..797791 (-) 1992 WP_321349988.1 biofilm regulation protein phosphatase SiaA -

Sequence

Protein


Download         Length: 384 a.a.        Molecular weight: 42963.44 Da        Isoelectric Point: 6.2920

>NTDB_id=1161844 ACKPCU_RS03635 WP_321349984.1 792834..793988(+) (pilU) [MAG: Halopseudomonas oceani isolate ab883223-6a80-4053-bf73-fb395571e785]
MEFEKLLRLMVEKGASDLFITAGVPPSMKVNGKILPVTKTPLSPEQTRETVLGVMTEAQRREFAEKRECNFAISARGIGR
FRVSSFYQRNLVGMVLRRIEINIPTMEDLRLPEVLKQLAMTKRGLVIFVGATGTGKSTSLASMIGYRNKNSSGHIITIED
PIEFIHQHQNCIVTQREVGIDTDSFEVALKNTLRQAPDVILIGEVRSKETMDHAVAFAETGHLCLATLHANNANQALDRI
IHFFPNEMHDQVWMDLSLNLKAIVAQQLIPTPDGKGRRAAIEVMLNTPLAADLIRKGEVHELKPLMARSNELGMQTFDQA
LYKLYSAGEITYEDALLHADSANDLRLMIKLGSETDGQHLMEDQLSNGLSLEEEEEVASRTRRF

Nucleotide


Download         Length: 1155 bp        

>NTDB_id=1161844 ACKPCU_RS03635 WP_321349984.1 792834..793988(+) (pilU) [MAG: Halopseudomonas oceani isolate ab883223-6a80-4053-bf73-fb395571e785]
ATGGAGTTCGAGAAGCTGTTGCGGTTGATGGTCGAGAAGGGCGCGTCCGACCTTTTTATTACCGCCGGTGTACCGCCGAG
CATGAAGGTCAACGGCAAGATTCTGCCGGTTACCAAAACCCCGCTTTCACCGGAACAGACCCGCGAAACCGTGCTGGGCG
TGATGACCGAAGCACAGCGCCGCGAGTTTGCCGAGAAGCGTGAGTGCAACTTCGCCATCAGCGCACGTGGTATTGGTCGC
TTCCGGGTCAGCTCGTTCTACCAGCGCAATCTGGTGGGTATGGTGCTGCGTCGGATCGAGATCAACATCCCGACCATGGA
AGATCTGCGCTTGCCGGAGGTGCTCAAGCAACTGGCCATGACCAAGCGTGGTCTGGTCATCTTCGTTGGTGCCACCGGGA
CCGGTAAGTCGACCTCGCTGGCGTCGATGATTGGCTATCGCAACAAGAATTCCAGCGGCCATATTATTACCATCGAAGAC
CCGATCGAATTCATTCATCAGCACCAGAACTGCATCGTGACCCAGCGCGAGGTTGGTATCGATACCGACTCGTTTGAGGT
CGCACTGAAGAACACCCTGCGGCAGGCGCCGGACGTGATTCTGATCGGTGAGGTGCGGTCCAAGGAGACCATGGACCACG
CGGTTGCCTTCGCTGAAACCGGTCACCTGTGTCTGGCAACCCTGCACGCCAACAATGCCAACCAGGCGCTGGACCGGATC
ATTCACTTTTTCCCGAATGAGATGCACGATCAGGTCTGGATGGACCTGTCGCTAAACCTCAAGGCCATCGTTGCCCAGCA
ATTGATCCCGACACCAGACGGCAAGGGGCGTCGCGCGGCAATTGAGGTGATGCTCAATACCCCGCTGGCAGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTCAAGCCACTGATGGCGCGCTCGAACGAGCTGGGCATGCAGACCTTCGATCAGGCG
TTGTACAAGCTCTACAGCGCAGGGGAGATCACCTATGAAGATGCGCTGCTGCACGCTGACTCGGCGAACGACCTGCGTCT
GATGATCAAGCTGGGGTCCGAGACCGACGGCCAGCACCTGATGGAAGATCAGCTCAGTAACGGGCTATCGCTGGAGGAAG
AGGAAGAGGTCGCCTCACGCACGCGCCGCTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(110-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

84.856

99.74

0.846
  pilU Acinetobacter baylyi ADP1

66.387

92.969

0.617
  pilU Vibrio cholerae strain A1552

60

91.146

0.547
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.543

88.281

0.393
  pilT Pseudomonas aeruginosa PAK

41.493

87.24

0.362
  pilT Legionella pneumophila strain Lp02

41.493

87.24

0.362
  pilT Legionella pneumophila strain ERS1305867

41.493

87.24

0.362

Multiple sequence alignment