Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACLMBN_RS11315 Genome accession   NZ_OY760626
Coordinates   2411526..2412563 (-) Length   345 a.a.
NCBI ID   WP_012729193.1    Uniprot ID   C4LCN0
Organism   MAG: Tolumonas auensis isolate c7031faa-31f6-4012-988d-549758c2b37d     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2406526..2417563
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLMBN_RS11290 (U2939_RS11255) fldB 2406828..2407346 (+) 519 WP_321406555.1 flavodoxin FldB -
  ACLMBN_RS11295 (U2939_RS11260) - 2407354..2408067 (-) 714 WP_041609468.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  ACLMBN_RS11300 (U2939_RS11265) srmB 2408173..2409489 (+) 1317 WP_321406559.1 ATP-dependent RNA helicase SrmB -
  ACLMBN_RS11305 (U2939_RS11270) yaaA 2409552..2410334 (-) 783 WP_321406561.1 peroxide stress protein YaaA -
  ACLMBN_RS11310 (U2939_RS11275) pilU 2410409..2411515 (-) 1107 WP_012729194.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACLMBN_RS11315 (U2939_RS11280) pilT 2411526..2412563 (-) 1038 WP_012729193.1 type IV pilus twitching motility protein PilT Machinery gene
  ACLMBN_RS11320 (U2939_RS11285) - 2412597..2413298 (+) 702 WP_012729192.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACLMBN_RS11325 (U2939_RS11290) proC 2413304..2414128 (+) 825 WP_321406566.1 pyrroline-5-carboxylate reductase -
  ACLMBN_RS11330 (U2939_RS11295) - 2414137..2414688 (+) 552 WP_012729190.1 YggT family protein -
  ACLMBN_RS11335 (U2939_RS11300) yggU 2414688..2414978 (+) 291 WP_012729189.1 DUF167 family protein YggU -
  ACLMBN_RS11340 (U2939_RS11305) rdgB 2415072..2415662 (+) 591 WP_321406570.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  ACLMBN_RS11345 (U2939_RS11310) hemW 2415666..2416811 (+) 1146 WP_321406572.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38341.18 Da        Isoelectric Point: 6.6929

>NTDB_id=1161518 ACLMBN_RS11315 WP_012729193.1 2411526..2412563(-) (pilT) [MAG: Tolumonas auensis isolate c7031faa-31f6-4012-988d-549758c2b37d]
MDITELLAFGVKHNASDLHLSAGLPPMIRVDGDVRRINVPALEHRQVHSLVYDIMNDHQRKVFEEDLEVDFSFEIPNLAR
FRVNAFNQSRGVAAAFRTIPSKVLTLDELGAPPIFRDIAENPRGLVLVTGPTGSGKSTTLAAMIDYINDHYNHHIITIED
PIEFVHQSKQCLVNQREVFRDTKSFNAALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPGEEKAMVRSMLSESMRAVISQTLMKKNGGGRLAAHEIMIGIPAIRNLIREDKVAQMYSVIQTGMVHGMQTMDYCL
KNLVSRGLISVQDAKAKAVDPNSIL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1161518 ACLMBN_RS11315 WP_012729193.1 2411526..2412563(-) (pilT) [MAG: Tolumonas auensis isolate c7031faa-31f6-4012-988d-549758c2b37d]
ATGGATATAACCGAATTATTGGCCTTTGGTGTTAAACATAATGCATCAGACTTGCATCTGTCTGCCGGGTTGCCACCTAT
GATTCGTGTTGATGGCGATGTCCGGCGTATCAATGTACCGGCGTTAGAACATCGGCAGGTGCACAGTCTGGTATATGACA
TCATGAACGACCATCAGCGTAAGGTATTTGAAGAGGATTTGGAAGTCGACTTCTCGTTTGAGATACCGAATCTGGCTCGT
TTCCGTGTGAATGCATTTAACCAGAGCCGGGGTGTGGCCGCTGCGTTCCGTACCATTCCGAGTAAGGTGCTGACGCTTGA
TGAACTGGGCGCTCCGCCGATATTCAGAGATATTGCTGAAAATCCAAGAGGGTTGGTTCTGGTTACCGGCCCGACAGGTT
CTGGTAAATCCACCACACTGGCAGCGATGATCGATTACATTAACGATCACTATAATCACCATATTATTACCATCGAAGAT
CCTATCGAATTCGTCCATCAGAGCAAGCAGTGTCTGGTTAACCAGCGTGAAGTGTTCCGTGATACCAAGAGCTTTAATGC
GGCACTGCGTTCCGCGTTACGTGAAGATCCGGATATCATTCTGGTCGGTGAAATGCGAGATCTGGAAACCATTCGTCTGG
CACTGACTGCCGCGGAAACCGGTCACCTGGTGTTTGGTACTCTGCATACCACCTCTGCGGCAAAAACCATCGACCGTATT
ATCGACGTCTTCCCCGGCGAAGAAAAAGCGATGGTCCGTTCTATGTTGTCAGAATCCATGCGAGCCGTTATTTCCCAGAC
GCTGATGAAGAAAAACGGCGGCGGCCGTCTTGCTGCGCATGAGATCATGATCGGTATTCCGGCAATCCGGAACCTTATCC
GTGAAGATAAGGTGGCTCAAATGTACTCAGTTATTCAGACCGGGATGGTGCATGGTATGCAAACCATGGACTATTGTTTA
AAGAATCTGGTTTCGCGTGGTTTGATTTCTGTTCAGGACGCTAAAGCGAAGGCTGTTGATCCTAACTCCATACTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C4LCN0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

78.841

100

0.788
  pilT Acinetobacter baumannii D1279779

78.551

100

0.786
  pilT Acinetobacter baumannii strain A118

78.551

100

0.786
  pilT Pseudomonas aeruginosa PAK

78.198

99.71

0.78
  pilT Pseudomonas stutzeri DSM 10701

77.907

99.71

0.777
  pilT Acinetobacter baylyi ADP1

77.035

99.71

0.768
  pilT Legionella pneumophila strain Lp02

72.647

98.551

0.716
  pilT Legionella pneumophila strain ERS1305867

72.647

98.551

0.716
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.92

97.681

0.693
  pilT Vibrio cholerae strain A1552

70.92

97.681

0.693
  pilT Neisseria meningitidis 8013

68.116

100

0.681
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.678
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.478

99.42

0.522
  pilU Vibrio cholerae strain A1552

40.87

100

0.409
  pilU Pseudomonas stutzeri DSM 10701

40

100

0.4
  pilU Acinetobacter baylyi ADP1

39.595

100

0.397

Multiple sequence alignment