Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACLMBN_RS11310 Genome accession   NZ_OY760626
Coordinates   2410409..2411515 (-) Length   368 a.a.
NCBI ID   WP_012729194.1    Uniprot ID   C4LCN1
Organism   MAG: Tolumonas auensis isolate c7031faa-31f6-4012-988d-549758c2b37d     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2405409..2416515
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLMBN_RS11285 (U2939_RS11250) xerD 2405856..2406749 (-) 894 WP_411354406.1 site-specific tyrosine recombinase XerD -
  ACLMBN_RS11290 (U2939_RS11255) fldB 2406828..2407346 (+) 519 WP_321406555.1 flavodoxin FldB -
  ACLMBN_RS11295 (U2939_RS11260) - 2407354..2408067 (-) 714 WP_041609468.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  ACLMBN_RS11300 (U2939_RS11265) srmB 2408173..2409489 (+) 1317 WP_321406559.1 ATP-dependent RNA helicase SrmB -
  ACLMBN_RS11305 (U2939_RS11270) yaaA 2409552..2410334 (-) 783 WP_321406561.1 peroxide stress protein YaaA -
  ACLMBN_RS11310 (U2939_RS11275) pilU 2410409..2411515 (-) 1107 WP_012729194.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACLMBN_RS11315 (U2939_RS11280) pilT 2411526..2412563 (-) 1038 WP_012729193.1 type IV pilus twitching motility protein PilT Machinery gene
  ACLMBN_RS11320 (U2939_RS11285) - 2412597..2413298 (+) 702 WP_012729192.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACLMBN_RS11325 (U2939_RS11290) proC 2413304..2414128 (+) 825 WP_321406566.1 pyrroline-5-carboxylate reductase -
  ACLMBN_RS11330 (U2939_RS11295) - 2414137..2414688 (+) 552 WP_012729190.1 YggT family protein -
  ACLMBN_RS11335 (U2939_RS11300) yggU 2414688..2414978 (+) 291 WP_012729189.1 DUF167 family protein YggU -
  ACLMBN_RS11340 (U2939_RS11305) rdgB 2415072..2415662 (+) 591 WP_321406570.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41020.09 Da        Isoelectric Point: 5.4642

>NTDB_id=1161517 ACLMBN_RS11310 WP_012729194.1 2410409..2411515(-) (pilU) [MAG: Tolumonas auensis isolate c7031faa-31f6-4012-988d-549758c2b37d]
MELANLLRILVDEKGSDLFISVGIQPSLKVNGKIRRLGDQLLEEDEVLNLVRQTMTDERFRSYVENREANFAINCPGLGR
FRVSAFWQQDCPGCSIRRIETVIPTCDELFLPMSVKELAMAKRGLILFVGATGAGKSTTQAAMIGYRNSHANDHILTIED
PVEFVHKHDRSLITQREVGSDTLSFDEGLKSALRQAPDVILIGEIRSEETMEFALSFAETGHLCMATLHANNANQAIERI
LHLVPAEKHRLLMYDLAFNLKAIVAQQLVPTIDGKSRRAAFEIMLNSPLISDIMRKGEVHRLKETMSRSRELGMSTFDQS
LFELYQQGAIGFDEALAFADSSNEVRVMIKMAAGGNFDSGMLDNVTVA

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=1161517 ACLMBN_RS11310 WP_012729194.1 2410409..2411515(-) (pilU) [MAG: Tolumonas auensis isolate c7031faa-31f6-4012-988d-549758c2b37d]
ATGGAACTGGCTAATTTATTGCGCATTTTGGTTGATGAAAAAGGGTCGGATTTATTTATTTCTGTCGGTATCCAGCCTTC
ACTTAAGGTGAACGGTAAGATCCGTCGTCTGGGCGATCAACTGCTGGAGGAAGATGAAGTTCTTAATCTGGTTCGCCAGA
CGATGACGGATGAGCGTTTTCGTTCATATGTGGAAAACAGAGAAGCGAACTTTGCGATCAATTGTCCCGGATTAGGCCGG
TTCCGTGTCAGTGCTTTCTGGCAGCAGGATTGCCCGGGGTGCTCTATCCGGCGTATCGAGACGGTGATCCCAACCTGTGA
TGAGCTGTTTTTGCCAATGTCGGTTAAAGAACTGGCAATGGCAAAGCGCGGGTTGATCCTGTTTGTTGGTGCGACCGGTG
CGGGTAAATCGACGACGCAGGCGGCAATGATTGGTTACCGTAACAGTCATGCGAATGACCATATTCTGACGATTGAAGAC
CCGGTCGAATTCGTGCATAAGCATGATCGCAGTCTGATCACGCAGCGTGAGGTCGGCTCGGATACGCTCTCGTTTGATGA
AGGTCTGAAAAGTGCGTTACGCCAGGCTCCGGACGTTATTCTGATCGGTGAAATTCGTTCTGAAGAGACCATGGAATTTG
CCTTATCGTTTGCGGAAACCGGTCATCTTTGCATGGCCACACTGCATGCGAATAATGCAAACCAGGCCATAGAACGTATT
CTTCACTTGGTGCCGGCAGAAAAGCATCGCCTGCTGATGTACGATTTGGCATTCAACTTAAAAGCTATTGTGGCGCAGCA
ACTGGTGCCGACGATCGATGGTAAAAGCCGGCGGGCTGCATTTGAGATTATGCTTAATTCACCGCTGATCAGTGACATTA
TGCGTAAAGGTGAAGTGCATCGTCTGAAAGAGACGATGTCCCGTTCCCGTGAACTGGGCATGTCTACGTTTGATCAATCT
TTGTTTGAGTTGTATCAGCAAGGTGCGATTGGCTTTGATGAAGCGCTGGCGTTTGCAGATTCAAGCAATGAAGTCCGCGT
CATGATCAAGATGGCTGCTGGTGGTAACTTTGACTCTGGCATGCTGGATAACGTGACCGTCGCTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C4LCN1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

55.858

99.728

0.557
  pilU Pseudomonas stutzeri DSM 10701

57.224

95.924

0.549
  pilU Acinetobacter baylyi ADP1

54.902

97.011

0.533
  pilT Acinetobacter baylyi ADP1

42.522

92.663

0.394
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.563

96.467

0.391
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.667

91.304

0.38
  pilT Vibrio cholerae strain A1552

41.667

91.304

0.38
  pilT Legionella pneumophila strain Lp02

41.692

89.946

0.375
  pilT Legionella pneumophila strain ERS1305867

41.692

89.946

0.375
  pilT Neisseria gonorrhoeae MS11

40.058

92.935

0.372
  pilT Neisseria meningitidis 8013

40.058

92.935

0.372
  pilT Acinetobacter nosocomialis M2

41.212

89.674

0.37
  pilT Acinetobacter baumannii D1279779

41.212

89.674

0.37
  pilT Acinetobacter baumannii strain A118

41.212

89.674

0.37
  pilT Pseudomonas aeruginosa PAK

40

91.033

0.364

Multiple sequence alignment