Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACORJQ_RS11550 Genome accession   NZ_OY759719
Coordinates   2549420..2550457 (+) Length   345 a.a.
NCBI ID   WP_321324686.1    Uniprot ID   -
Organism   MAG: Thiomicrorhabdus sp. isolate cfc1d0dd-18bb-48af-b12b-b905256c9fab     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2544420..2555457
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACORJQ_RS11530 (U2926_RS11515) lptG 2544866..2545951 (-) 1086 WP_321324679.1 LPS export ABC transporter permease LptG -
  ACORJQ_RS11535 (U2926_RS11520) lptF 2545948..2547069 (-) 1122 WP_321324681.1 LPS export ABC transporter permease LptF -
  ACORJQ_RS11540 (U2926_RS11525) - 2547202..2548698 (+) 1497 WP_321324682.1 leucyl aminopeptidase -
  ACORJQ_RS11545 (U2926_RS11530) - 2548709..2549179 (+) 471 WP_321324684.1 DNA polymerase III subunit chi -
  ACORJQ_RS11550 (U2926_RS11535) pilT 2549420..2550457 (+) 1038 WP_321324686.1 type IV pilus twitching motility protein PilT Machinery gene
  ACORJQ_RS11555 (U2926_RS11540) pilU 2550557..2551714 (+) 1158 WP_420719583.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACORJQ_RS11560 (U2926_RS11545) - 2551865..2552836 (+) 972 WP_321324690.1 DUF2817 domain-containing protein -
  ACORJQ_RS11565 (U2926_RS11550) - 2552970..2554094 (-) 1125 WP_321324692.1 L,D-transpeptidase -
  ACORJQ_RS11570 (U2926_RS11555) - 2554081..2554896 (-) 816 WP_321324693.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38409.07 Da        Isoelectric Point: 5.6625

>NTDB_id=1161455 ACORJQ_RS11550 WP_321324686.1 2549420..2550457(+) (pilT) [MAG: Thiomicrorhabdus sp. isolate cfc1d0dd-18bb-48af-b12b-b905256c9fab]
MDITQLLEFSVQQGASDLHLSTGQPPIIRIDGDVQRIEAPDLEPEQLQTMIYDIMNDEQRRGFESELESDFSFELPGIAR
FRANVFIHNRGIGAVFRTIPSKIITLEQLEAPEIFKAISSFPRGLVLVTGPTGSGKSTTLAAMIDYINNRDAAHILTVED
PIEFVHKPIRCLINQREVHRDTKSFTNALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLQAVISQALLKKTGGGRIAAHEIMLVNSAIRNLIREAKIPQMYSVIQTSKAIGMQTIDQNL
QDLVARNLISRDDARSKAVNKTLFQ

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1161455 ACORJQ_RS11550 WP_321324686.1 2549420..2550457(+) (pilT) [MAG: Thiomicrorhabdus sp. isolate cfc1d0dd-18bb-48af-b12b-b905256c9fab]
ATGGATATTACCCAATTACTTGAATTTAGTGTGCAGCAAGGGGCCTCGGATTTACACCTTTCTACAGGCCAGCCCCCTAT
TATTCGTATTGATGGTGATGTACAACGTATTGAAGCCCCCGACTTAGAACCAGAACAATTACAAACCATGATTTACGACA
TTATGAATGATGAACAACGTCGTGGTTTTGAATCGGAACTTGAATCCGATTTCTCATTTGAACTTCCTGGTATTGCTCGA
TTCAGAGCAAACGTATTTATCCATAACCGTGGTATAGGTGCCGTTTTTCGTACCATTCCAAGTAAAATCATCACGCTAGA
ACAACTTGAAGCTCCTGAGATTTTTAAAGCAATCTCTAGCTTTCCTCGTGGTTTGGTTTTAGTTACTGGCCCTACCGGGT
CTGGTAAATCAACAACCTTGGCCGCCATGATTGACTATATCAATAACCGAGATGCAGCTCATATTCTAACGGTAGAAGAT
CCAATCGAATTTGTTCACAAACCCATTCGCTGCTTGATTAACCAACGTGAAGTACATCGTGATACCAAAAGTTTTACCAA
CGCTTTACGCTCGGCGCTTCGTGAAGACCCCGATGTTATTCTAGTTGGTGAGATGCGTGACCTTGAAACCATTCGTTTAG
CCCTTACTGCAGCTGAAACGGGTCACTTGGTATTTGGTACTTTACATACCAGTTCTGCGGCCAAAACCATTGACCGTATT
ATTGATGTTTTCCCTGCTGAAGAAAAAGAAATGGTTCGTGCCATGCTGTCTGAATCTTTGCAAGCGGTTATCTCTCAAGC
CCTTCTTAAAAAGACTGGCGGTGGGCGTATTGCCGCACATGAAATAATGTTGGTTAATTCAGCCATACGTAATCTAATTC
GTGAGGCAAAAATACCTCAAATGTACTCTGTTATTCAAACGTCAAAGGCAATTGGCATGCAAACCATTGACCAAAATCTT
CAAGATTTAGTGGCTCGCAACTTAATTTCTCGCGATGACGCTCGCTCCAAAGCAGTGAATAAAACACTCTTTCAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

72.781

97.971

0.713
  pilT Acinetobacter baumannii strain A118

72.485

97.971

0.71
  pilT Acinetobacter baumannii D1279779

72.485

97.971

0.71
  pilT Acinetobacter nosocomialis M2

72.189

97.971

0.707
  pilT Pseudomonas stutzeri DSM 10701

72.189

97.971

0.707
  pilT Acinetobacter baylyi ADP1

72.189

97.971

0.707
  pilT Legionella pneumophila strain Lp02

67.733

99.71

0.675
  pilT Legionella pneumophila strain ERS1305867

67.733

99.71

0.675
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.359

97.681

0.658
  pilT Vibrio cholerae strain A1552

67.359

97.681

0.658
  pilT Neisseria meningitidis 8013

65.396

98.841

0.646
  pilT Neisseria gonorrhoeae MS11

65.103

98.841

0.643
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

100

0.507
  pilU Vibrio cholerae strain A1552

41.003

98.261

0.403
  pilU Pseudomonas stutzeri DSM 10701

40

97.101

0.388
  pilU Acinetobacter baylyi ADP1

39.118

98.551

0.386

Multiple sequence alignment