Detailed information NTG1161456

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACORJQ_RS11555
• Genome accession: NZ_OY759719
• Coordinates: 2550557..2551714 (+)
• Length: 385 a.a.
• NCBI ID: WP_420719583.1
• Uniprot ID: -
• Organism: MAG: Thiomicrorhabdus sp. isolate cfc1d0dd-18bb-48af-b12b-b905256c9fab
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2545557..2556714

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACORJQ_RS11535 (U2926_RS11520)	lptF	2545948..2547069 (-)	1122	WP_321324681.1	LPS export ABC transporter permease LptF	-
ACORJQ_RS11540 (U2926_RS11525)	-	2547202..2548698 (+)	1497	WP_321324682.1	leucyl aminopeptidase	-
ACORJQ_RS11545 (U2926_RS11530)	-	2548709..2549179 (+)	471	WP_321324684.1	DNA polymerase III subunit chi	-
ACORJQ_RS11550 (U2926_RS11535)	pilT	2549420..2550457 (+)	1038	WP_321324686.1	type IV pilus twitching motility protein PilT	Machinery gene
ACORJQ_RS11555 (U2926_RS11540)	pilU	2550557..2551714 (+)	1158	WP_420719583.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACORJQ_RS11560 (U2926_RS11545)	-	2551865..2552836 (+)	972	WP_321324690.1	DUF2817 domain-containing protein	-
ACORJQ_RS11565 (U2926_RS11550)	-	2552970..2554094 (-)	1125	WP_321324692.1	L,D-transpeptidase	-
ACORJQ_RS11570 (U2926_RS11555)	-	2554081..2554896 (-)	816	WP_321324693.1	hypothetical protein	-

Sequence

Protein

• Length: 385 a.a.
• Molecular weight: 42776.72 Da
• Isoelectric Point: 6.1307

>NTDB_id=1161456 ACORJQ_RS11555 WP_420719583.1 2550557..2551714(+) (pilU) [MAG: Thiomicrorhabdus sp. isolate cfc1d0dd-18bb-48af-b12b-b905256c9fab]
MNQQENLTELQKLLVFFSQKGGSDLFITAGRPATMKKDGKLISLTEDRLTAEASQALAYQIMNHDQLATFQNTQESNFAY
HISGIARYRVNVFMQRGTPGMVIRSVHTEIPKFEDLNLPAILKEVIMDKHGLVLIVGGTGSGKSTTLASLVDVRNSTRDG
HIITIEDPIEFMHSHKKSIVTQREVGVDTDSYDSALKNTLRQAPDVILIGEIRSRDTMEHAIAFSETGHLCLSTLHANNT
NQTLDRIINFFPADQRQHILMDLSLNLKAIVSQRLIPKIGGGQIAAVEVLINTPRIAELIFSGKVNEIKQLMQNSSALGM
QTFDQALFDLYENGLITYKDALRGADSMNDLRLNIKLNSKLPQPQDEESDLASDAISFSLKQDNN

Nucleotide

• Length: 1158 bp

>NTDB_id=1161456 ACORJQ_RS11555 WP_420719583.1 2550557..2551714(+) (pilU) [MAG: Thiomicrorhabdus sp. isolate cfc1d0dd-18bb-48af-b12b-b905256c9fab]
ATGAACCAGCAAGAAAACTTGACCGAACTTCAAAAACTGCTCGTCTTTTTTAGTCAAAAAGGTGGCTCTGATCTGTTCAT
TACAGCAGGTCGTCCAGCGACAATGAAAAAAGACGGCAAGTTAATTTCGCTAACAGAAGATAGACTGACAGCAGAAGCTT
CACAAGCTTTAGCTTACCAGATTATGAATCATGATCAGCTTGCAACCTTTCAAAACACACAAGAAAGTAACTTTGCCTAC
CATATCAGTGGGATTGCTCGCTACCGTGTAAATGTATTTATGCAACGTGGAACACCAGGCATGGTCATTCGTTCGGTACA
TACCGAAATCCCTAAATTTGAAGACTTAAACTTACCGGCCATATTGAAAGAAGTCATTATGGATAAACACGGCTTAGTGT
TAATCGTTGGGGGTACAGGTTCTGGTAAATCAACCACTCTTGCTAGTTTGGTTGATGTACGAAACTCAACCCGAGATGGT
CATATCATTACCATTGAAGACCCAATTGAATTTATGCACAGCCATAAAAAGAGTATTGTGACTCAACGCGAAGTAGGCGT
AGATACCGACAGTTACGACAGTGCTCTTAAAAACACACTAAGACAAGCACCCGATGTTATCTTAATTGGTGAGATTCGAA
GTCGAGACACCATGGAACACGCCATTGCTTTCTCTGAAACGGGTCACTTGTGTTTATCTACTTTGCATGCCAACAACACT
AACCAAACCTTAGACCGAATTATTAACTTCTTCCCTGCTGACCAACGTCAGCATATATTGATGGATTTATCGCTTAACCT
TAAAGCAATCGTATCACAAAGATTGATTCCTAAAATCGGTGGAGGCCAAATTGCTGCAGTAGAAGTTTTAATCAATACTC
CACGTATTGCAGAACTGATTTTTAGCGGTAAAGTAAATGAAATCAAACAACTCATGCAAAACTCAAGTGCGTTGGGAATG
CAAACCTTTGACCAAGCTCTATTTGATCTGTATGAAAATGGCTTAATTACCTACAAAGACGCACTTAGAGGTGCCGATTC
TATGAATGATTTACGCCTAAATATCAAATTAAATAGTAAACTGCCACAACCCCAAGATGAGGAAAGTGACTTAGCTTCAG
ACGCTATTTCATTTAGCTTAAAACAAGATAACAATTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (22-277)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	59.207	91.688	0.543
pilU	Acinetobacter baylyi ADP1	55.337	92.468	0.512
pilU	Vibrio cholerae strain A1552	53.869	87.273	0.47
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.308	87.792	0.371
pilT	Legionella pneumophila strain ERS1305867	41.543	87.532	0.364
pilT	Legionella pneumophila strain Lp02	41.543	87.532	0.364
pilT	Pseudomonas aeruginosa PAK	41.742	86.494	0.361