Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   R2K28_RS08685 Genome accession   NZ_OY734020
Coordinates   1916527..1917561 (+) Length   344 a.a.
NCBI ID   WP_316369196.1    Uniprot ID   -
Organism   Candidatus Thiodiazotropha sp. CDECU1 isolate 46184     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1911527..1922561
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R2K28_RS08660 - 1911722..1913698 (-) 1977 WP_316369188.1 dynamin family protein -
  R2K28_RS08665 - 1913807..1914091 (-) 285 WP_116445670.1 DUF167 family protein -
  R2K28_RS08670 - 1914088..1914666 (-) 579 WP_316369191.1 YggT family protein -
  R2K28_RS08675 proC 1914668..1915495 (-) 828 WP_316369193.1 pyrroline-5-carboxylate reductase -
  R2K28_RS08680 - 1915533..1916240 (-) 708 WP_316369195.1 YggS family pyridoxal phosphate-dependent enzyme -
  R2K28_RS08685 pilT 1916527..1917561 (+) 1035 WP_316369196.1 type IV pilus twitching motility protein PilT Machinery gene
  R2K28_RS08690 pilU 1917685..1918809 (+) 1125 WP_316369197.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  R2K28_RS08695 - 1918855..1919319 (-) 465 WP_316369198.1 hypothetical protein -
  R2K28_RS08700 - 1919487..1920062 (-) 576 WP_316369200.1 ankyrin repeat domain-containing protein -
  R2K28_RS08705 - 1920118..1920453 (-) 336 WP_316369202.1 DUF1820 family protein -
  R2K28_RS08710 - 1920732..1921520 (+) 789 WP_316369204.1 hypothetical protein -
  R2K28_RS08715 - 1921665..1922138 (+) 474 WP_316369206.1 DsrE family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38039.77 Da        Isoelectric Point: 6.7748

>NTDB_id=1160757 R2K28_RS08685 WP_316369196.1 1916527..1917561(+) (pilT) [Candidatus Thiodiazotropha sp. CDECU1 isolate 46184]
MDIAELLAFSVKHNASDLHLSAGLPPMIRVDGDIRRINVPALEHKVVHALVYDIMNDKQRKDFEEYLENDFSFEIPGLAR
FRVNAFNQARGASAVFRTIPSKVLTLEELGCPTTFKEIVDVPRGLVLVTGPTGSGKSTTLAAMMDYKNDNDYSHILTIED
PIEFVHSSKKCLINQREVHRDTLGFSEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKSMVRSMLSESLRSVIAQTLLKKIGGGRIAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQAAGMQTMDQAL
KELVQRGVVSRLDAKAKAQNKDQF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1160757 R2K28_RS08685 WP_316369196.1 1916527..1917561(+) (pilT) [Candidatus Thiodiazotropha sp. CDECU1 isolate 46184]
ATGGATATCGCTGAACTTCTGGCCTTCAGTGTCAAGCACAACGCATCAGACTTGCATCTCTCTGCCGGTTTGCCACCCAT
GATTCGTGTGGATGGCGACATCAGACGCATCAATGTCCCCGCTCTTGAACACAAGGTCGTTCACGCCCTTGTGTATGACA
TCATGAATGATAAACAGCGCAAGGACTTCGAGGAGTACCTGGAGAATGACTTCTCCTTCGAGATTCCCGGATTGGCGAGA
TTTCGTGTCAACGCCTTCAATCAGGCCCGCGGCGCATCGGCGGTCTTTCGTACCATCCCATCCAAGGTGCTGACCCTGGA
AGAGCTCGGCTGTCCCACTACCTTCAAGGAAATCGTGGATGTGCCCCGCGGGCTGGTGCTGGTGACCGGACCGACCGGCT
CCGGTAAGTCCACCACCCTGGCTGCCATGATGGATTACAAGAACGACAATGACTACTCCCATATCCTCACCATCGAGGAT
CCGATCGAGTTTGTTCACTCCAGCAAGAAGTGCCTGATCAACCAGCGCGAAGTGCATCGTGACACCTTGGGATTTTCCGA
GGCGCTGCGCTCTGCTTTGCGTGAAGACCCGGATATCATCCTGGTGGGCGAGCTGCGTGATCTGGAAACCATCCGCCTGG
CCTTGACCGCCGCCGAAACCGGACATCTGGTATTCGGTACCCTGCACACCAGTTCGGCGGCCAAGACCATCGACCGGATC
ATCGATGTCTTTCCGGCGGGAGAGAAGTCGATGGTGCGATCCATGTTGTCCGAATCCCTGCGATCCGTGATTGCCCAGAC
CCTGCTGAAAAAGATTGGTGGTGGTCGAATTGCTGCTTGGGAGATCATGGTTGGCACCCCGGCGATCCGAAACCTGATCC
GTGAGGACAAGGTCGCGCAGATGTATTCCGCGATCCAGACCGGACAGGCAGCGGGGATGCAGACCATGGACCAGGCCCTG
AAGGAGTTGGTGCAGCGTGGTGTGGTAAGCCGTCTTGACGCCAAGGCCAAGGCGCAAAACAAGGATCAGTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

81.686

100

0.817
  pilT Pseudomonas stutzeri DSM 10701

80.523

100

0.805
  pilT Acinetobacter nosocomialis M2

79.07

100

0.791
  pilT Acinetobacter baumannii D1279779

79.07

100

0.791
  pilT Acinetobacter baumannii strain A118

79.07

100

0.791
  pilT Acinetobacter baylyi ADP1

76.744

100

0.767
  pilT Legionella pneumophila strain Lp02

74.709

100

0.747
  pilT Legionella pneumophila strain ERS1305867

74.709

100

0.747
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.566

98.547

0.715
  pilT Vibrio cholerae strain A1552

72.566

98.547

0.715
  pilT Neisseria meningitidis 8013

66.181

99.709

0.66
  pilT Neisseria gonorrhoeae MS11

65.889

99.709

0.657
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.145

100

0.503
  pilU Vibrio cholerae strain A1552

42.09

97.384

0.41
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.384

0.404
  pilU Acinetobacter baylyi ADP1

39.583

97.674

0.387

Multiple sequence alignment