Detailed information NTG1160758

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: R2K28_RS08690
• Genome accession: NZ_OY734020
• Coordinates: 1917685..1918809 (+)
• Length: 374 a.a.
• NCBI ID: WP_316369197.1
• Uniprot ID: -
• Organism: Candidatus Thiodiazotropha sp. CDECU1 isolate 46184
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1912685..1923809

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
R2K28_RS08665	-	1913807..1914091 (-)	285	WP_116445670.1	DUF167 family protein	-
R2K28_RS08670	-	1914088..1914666 (-)	579	WP_316369191.1	YggT family protein	-
R2K28_RS08675	proC	1914668..1915495 (-)	828	WP_316369193.1	pyrroline-5-carboxylate reductase	-
R2K28_RS08680	-	1915533..1916240 (-)	708	WP_316369195.1	YggS family pyridoxal phosphate-dependent enzyme	-
R2K28_RS08685	pilT	1916527..1917561 (+)	1035	WP_316369196.1	type IV pilus twitching motility protein PilT	Machinery gene
R2K28_RS08690	pilU	1917685..1918809 (+)	1125	WP_316369197.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
R2K28_RS08695	-	1918855..1919319 (-)	465	WP_316369198.1	hypothetical protein	-
R2K28_RS08700	-	1919487..1920062 (-)	576	WP_316369200.1	ankyrin repeat domain-containing protein	-
R2K28_RS08705	-	1920118..1920453 (-)	336	WP_316369202.1	DUF1820 family protein	-
R2K28_RS08710	-	1920732..1921520 (+)	789	WP_316369204.1	hypothetical protein	-
R2K28_RS08715	-	1921665..1922138 (+)	474	WP_316369206.1	DsrE family protein	-
R2K28_RS08720	-	1922245..1922802 (-)	558	WP_316369207.1	DUF2799 domain-containing protein	-
R2K28_RS08725	-	1923341..1923541 (-)	201	WP_116445661.1	hypothetical protein	-

Sequence

Protein

• Length: 374 a.a.
• Molecular weight: 41858.12 Da
• Isoelectric Point: 6.7403

>NTDB_id=1160758 R2K28_RS08690 WP_316369197.1 1917685..1918809(+) (pilU) [Candidatus Thiodiazotropha sp. CDECU1 isolate 46184]
MDLNALLSVVVKNKASDLFVTAGREPSIKVDGKIHPINKTPLTPRQTKEMVYSIMTDAQQKEFDETKECNFAISVKRLGR
FRVSSFYQRDTVGMVLRRIENKIPTLESLYLPPILQDLSMVKRGLVIFVGATGTGKSTSLAAMIGYRNTRGDGHIVSIED
PIEYMHDHNQCIITQREVGIDTESYEVALKNTLRQAPDVILIGEIRTRKTMEHAIAFAETGHLCLSTLHANNANQALDRV
IHFFPEDAREQIFMDLSLNLKAIVAQQLIPKVDGNGRRAAVEVMLNTPLAAELIRKGEIHKLKELMKRSTEHGMITFDQH
LFQLYEEGVISYENALAHADSTNDVRLMIKLGAAHTSDSLIDELDGITLSDGKH

Nucleotide

• Length: 1125 bp

>NTDB_id=1160758 R2K28_RS08690 WP_316369197.1 1917685..1918809(+) (pilU) [Candidatus Thiodiazotropha sp. CDECU1 isolate 46184]
ATGGATTTGAACGCACTTCTATCCGTGGTGGTGAAAAACAAGGCGTCCGATCTGTTCGTAACCGCTGGGCGAGAGCCCTC
CATAAAGGTGGATGGAAAGATCCATCCCATCAATAAGACCCCTTTGACACCCAGGCAGACCAAGGAGATGGTCTATAGCA
TCATGACCGATGCACAACAGAAGGAGTTCGACGAAACCAAAGAGTGCAATTTCGCCATCAGTGTCAAAAGATTGGGCCGC
TTCCGGGTCAGCAGTTTTTATCAGCGCGATACTGTAGGTATGGTACTACGCCGCATTGAAAACAAGATACCTACCCTGGA
ATCACTCTATCTACCCCCCATTCTGCAGGATCTCTCCATGGTCAAACGCGGCCTGGTGATCTTCGTGGGCGCTACCGGAA
CCGGTAAATCCACCTCACTGGCGGCCATGATCGGCTATCGCAACACCAGGGGCGATGGTCATATTGTCAGTATCGAAGAC
CCCATTGAGTACATGCACGATCACAACCAGTGCATCATTACGCAGCGTGAAGTGGGTATAGACACCGAGTCATATGAAGT
GGCGCTCAAGAACACTCTGCGCCAGGCGCCGGACGTTATTCTGATTGGTGAAATACGTACCCGAAAGACCATGGAGCATG
CCATTGCCTTTGCCGAAACCGGTCATCTATGCCTCTCGACCCTGCATGCCAACAACGCCAATCAGGCACTTGATCGTGTT
ATCCACTTCTTTCCGGAGGATGCCAGGGAGCAGATCTTTATGGATCTATCACTGAATCTGAAGGCCATAGTGGCTCAGCA
ACTGATCCCGAAAGTGGACGGCAACGGCCGGCGTGCCGCTGTCGAGGTGATGTTGAATACCCCCTTGGCCGCAGAGCTGA
TTCGCAAAGGTGAAATCCATAAGCTCAAAGAGTTGATGAAGCGTTCAACGGAACACGGCATGATCACTTTTGACCAGCAT
CTCTTCCAGCTTTATGAAGAGGGTGTCATCAGCTATGAGAATGCCCTGGCCCATGCCGATTCGACCAACGATGTACGCCT
GATGATAAAACTTGGTGCGGCCCATACCAGCGACTCCCTGATCGATGAGCTGGATGGGATTACCCTGTCGGATGGTAAGC
ACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (10-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	67.209	98.663	0.663
pilU	Acinetobacter baylyi ADP1	64.023	94.385	0.604
pilU	Vibrio cholerae strain A1552	56.857	93.583	0.532
pilT	Legionella pneumophila strain ERS1305867	42.388	89.572	0.38
pilT	Legionella pneumophila strain Lp02	42.388	89.572	0.38
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.593	90.642	0.377
pilT	Acinetobacter baylyi ADP1	41.349	91.176	0.377
pilT	Acinetobacter baumannii D1279779	41.791	89.572	0.374
pilT	Pseudomonas aeruginosa PAK	41.791	89.572	0.374
pilT	Acinetobacter nosocomialis M2	41.791	89.572	0.374
pilT	Acinetobacter baumannii strain A118	41.791	89.572	0.374
pilT	Vibrio cholerae strain A1552	41.212	88.235	0.364
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	41.212	88.235	0.364
pilT	Pseudomonas stutzeri DSM 10701	40.299	89.572	0.361