Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   THITH_RS15570 Genome accession   NZ_CP007029
Coordinates   3441576..3442610 (-) Length   344 a.a.
NCBI ID   WP_006746882.1    Uniprot ID   -
Organism   Thioalkalivibrio paradoxus ARh     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3436576..3447610
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  THITH_RS15550 (THITH_16020) - 3436822..3437829 (+) 1008 WP_006746886.1 aspartate carbamoyltransferase catalytic subunit -
  THITH_RS15555 (THITH_16025) - 3437826..3439118 (+) 1293 WP_006746885.1 dihydroorotase -
  THITH_RS15560 (THITH_16030) - 3439209..3440339 (-) 1131 WP_006746884.1 PilT/PilU family type 4a pilus ATPase -
  THITH_RS15565 (THITH_16035) pilU 3440347..3441519 (-) 1173 WP_006746883.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  THITH_RS15570 (THITH_16040) pilT 3441576..3442610 (-) 1035 WP_006746882.1 type IV pilus twitching motility protein PilT Machinery gene
  THITH_RS15575 (THITH_16045) - 3442719..3443432 (+) 714 WP_006746881.1 YggS family pyridoxal phosphate-dependent enzyme -
  THITH_RS15580 (THITH_16050) proC 3443429..3444247 (+) 819 WP_006746880.1 pyrroline-5-carboxylate reductase -
  THITH_RS15585 (THITH_16055) - 3444381..3444938 (+) 558 WP_006746879.1 YggT family protein -
  THITH_RS19465 - 3444942..3445133 (-) 192 WP_232222212.1 hypothetical protein -
  THITH_RS15590 (THITH_16060) - 3445266..3445718 (+) 453 WP_006746878.1 DUF4426 domain-containing protein -
  THITH_RS15595 (THITH_16065) - 3445727..3446392 (-) 666 WP_025367636.1 sulfite exporter TauE/SafE family protein -
  THITH_RS15600 (THITH_16070) hspQ 3446518..3446829 (-) 312 WP_006746876.1 heat shock protein HspQ -
  THITH_RS19470 - 3446913..3447143 (+) 231 WP_084222686.1 hypothetical protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38237.72 Da        Isoelectric Point: 6.6659

>NTDB_id=116069 THITH_RS15570 WP_006746882.1 3441576..3442610(-) (pilT) [Thioalkalivibrio paradoxus ARh]
MDITQLLSFAVKNGASDLHISAGMPPLVRIDGDMRRVNVPAMDHKQVQALIYDIMNDRQRKDYEEFWETDFSFELKGLAR
FRVNAFNQDRGAAAVFRTIPSKVLTLEDLGAPRVFEKISDYPRGIVLVTGPTGSGKSTTLAAMVNHKNDNEYGHILTIED
PIEFVHESKKCLVNQREVHRDTHGFSEALRSALREDPDTILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPAAEKDMVRAMLSESLRAVISQTLLKRVGGGRIAAHEIMLGTPAIRNLIRENKIAQMYSTIQTSANLGMQTLDQCL
KDMLAEGTITREDARRRAVNADAL

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=116069 THITH_RS15570 WP_006746882.1 3441576..3442610(-) (pilT) [Thioalkalivibrio paradoxus ARh]
ATGGACATTACCCAGTTGCTTTCCTTCGCGGTCAAGAACGGCGCATCGGACCTGCATATCTCGGCCGGCATGCCCCCGCT
GGTCCGCATCGATGGTGACATGCGCCGGGTCAACGTGCCCGCGATGGACCACAAGCAGGTCCAGGCGCTGATCTACGACA
TCATGAACGATCGTCAGCGCAAGGACTACGAGGAGTTCTGGGAAACCGATTTCTCGTTCGAGCTGAAGGGGCTTGCGCGG
TTCCGGGTCAATGCCTTCAACCAGGATCGGGGCGCGGCGGCCGTGTTCCGCACCATCCCGTCCAAGGTGCTGACGCTGGA
AGACCTCGGTGCGCCGCGGGTGTTCGAGAAGATTTCCGACTATCCGCGCGGCATCGTGCTGGTGACCGGGCCGACCGGGT
CCGGCAAGTCGACCACGCTCGCGGCAATGGTCAACCACAAGAACGACAACGAGTACGGCCACATCCTCACGATCGAGGAC
CCGATCGAATTCGTACACGAATCCAAGAAATGCCTGGTGAACCAGCGCGAGGTTCACCGCGACACGCATGGCTTCAGCGA
AGCGCTGCGCTCGGCGCTGCGCGAGGATCCGGACACGATCCTGGTCGGCGAGCTGCGCGATCTCGAAACCATTCGGCTGG
CGCTGACCGCGGCGGAAACCGGGCACTTGGTGTTTGGAACGCTGCACACCAGTTCTGCGGCCAAGACCATCGACCGGGTC
GTCGACGTCTTCCCGGCGGCGGAAAAGGACATGGTGCGCGCGATGCTGTCGGAATCGCTGCGCGCGGTGATCTCGCAAAC
GCTGCTGAAGCGCGTGGGCGGTGGCCGGATCGCCGCGCACGAGATCATGCTGGGGACGCCGGCGATCCGTAACCTGATCC
GCGAAAACAAGATCGCGCAGATGTACTCGACCATCCAGACCAGCGCCAATCTGGGCATGCAGACCCTCGACCAGTGCCTG
AAGGACATGCTTGCGGAAGGCACGATAACGCGCGAGGATGCCAGGCGTCGGGCCGTGAATGCCGACGCACTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

80.178

98.256

0.788
  pilT Pseudomonas aeruginosa PAK

78.402

98.256

0.77
  pilT Acinetobacter baumannii D1279779

76.744

100

0.767
  pilT Acinetobacter baumannii strain A118

76.744

100

0.767
  pilT Acinetobacter nosocomialis M2

76.453

100

0.765
  pilT Acinetobacter baylyi ADP1

74.419

100

0.744
  pilT Legionella pneumophila strain Lp02

70

98.837

0.692
  pilT Legionella pneumophila strain ERS1305867

70

98.837

0.692
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.733

97.965

0.683
  pilT Vibrio cholerae strain A1552

69.733

97.965

0.683
  pilT Neisseria meningitidis 8013

65.217

100

0.654
  pilT Neisseria gonorrhoeae MS11

64.928

100

0.651
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.147

99.128

0.497
  pilU Pseudomonas stutzeri DSM 10701

41.449

100

0.416
  pilU Vibrio cholerae strain A1552

40.58

100

0.407
  pilU Acinetobacter baylyi ADP1

40.173

100

0.404

Multiple sequence alignment