Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   THITH_RS15565 Genome accession   NZ_CP007029
Coordinates   3440347..3441519 (-) Length   390 a.a.
NCBI ID   WP_006746883.1    Uniprot ID   W0DQL0
Organism   Thioalkalivibrio paradoxus ARh     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3435347..3446519
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  THITH_RS15540 (THITH_16010) ruvX 3435925..3436326 (+) 402 WP_025367634.1 Holliday junction resolvase RuvX -
  THITH_RS15545 (THITH_16015) pyrR 3436319..3436825 (+) 507 WP_006746887.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  THITH_RS15550 (THITH_16020) - 3436822..3437829 (+) 1008 WP_006746886.1 aspartate carbamoyltransferase catalytic subunit -
  THITH_RS15555 (THITH_16025) - 3437826..3439118 (+) 1293 WP_006746885.1 dihydroorotase -
  THITH_RS15560 (THITH_16030) - 3439209..3440339 (-) 1131 WP_006746884.1 PilT/PilU family type 4a pilus ATPase -
  THITH_RS15565 (THITH_16035) pilU 3440347..3441519 (-) 1173 WP_006746883.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  THITH_RS15570 (THITH_16040) pilT 3441576..3442610 (-) 1035 WP_006746882.1 type IV pilus twitching motility protein PilT Machinery gene
  THITH_RS15575 (THITH_16045) - 3442719..3443432 (+) 714 WP_006746881.1 YggS family pyridoxal phosphate-dependent enzyme -
  THITH_RS15580 (THITH_16050) proC 3443429..3444247 (+) 819 WP_006746880.1 pyrroline-5-carboxylate reductase -
  THITH_RS15585 (THITH_16055) - 3444381..3444938 (+) 558 WP_006746879.1 YggT family protein -
  THITH_RS19465 - 3444942..3445133 (-) 192 WP_232222212.1 hypothetical protein -
  THITH_RS15590 (THITH_16060) - 3445266..3445718 (+) 453 WP_006746878.1 DUF4426 domain-containing protein -
  THITH_RS15595 (THITH_16065) - 3445727..3446392 (-) 666 WP_025367636.1 sulfite exporter TauE/SafE family protein -

Sequence

Protein


Download         Length: 390 a.a.        Molecular weight: 43681.94 Da        Isoelectric Point: 6.1390

>NTDB_id=116068 THITH_RS15565 WP_006746883.1 3440347..3441519(-) (pilU) [Thioalkalivibrio paradoxus ARh]
MDRDKAIRYMHDLLRTMVGRGGSDLFITVGMPPAAKINGLVQPMTDQNLTSAHTQVLVRSMMNDKQSAEFDRTHECNFAI
ALEGVSRFRVSAFTQRGNVGMVLRTIQGKIPSFEEINMPPQLKDIAMTRRGLVIFVGATSSGKSTSLAAMIGYRNHHSAD
HIITIEDPIEFVHAHDKSIVTQREVGVDTESYEIALKNTLRQAPDVILIGEIRDRATMDYGIAFAETGHLCLSTLHANNT
NQALDRIINFFPEERRPQLLMDLSLNLKAVVSQRLIRTQDGEGRVPAVEIMINTPLMSDLILKGAVHEMKDLIARSREQG
MQTFDQALFDLYEAGKISYQEAIRNADSMNDLRLRVKLDSKRAQREEEPDVMDDLSGLSVADKVDDFVAR

Nucleotide


Download         Length: 1173 bp        

>NTDB_id=116068 THITH_RS15565 WP_006746883.1 3440347..3441519(-) (pilU) [Thioalkalivibrio paradoxus ARh]
ATGGATCGTGACAAGGCCATACGTTACATGCACGACCTGTTGCGGACGATGGTGGGCCGTGGCGGGTCGGATCTGTTCAT
CACGGTAGGCATGCCGCCTGCGGCCAAGATCAATGGTCTGGTGCAGCCGATGACGGACCAGAACCTGACCTCGGCGCATA
CGCAGGTGCTGGTGCGCTCGATGATGAACGATAAGCAGTCAGCCGAGTTCGACCGCACCCATGAGTGCAATTTCGCGATC
GCCCTCGAGGGGGTGTCGCGTTTTCGCGTCAGCGCTTTTACCCAACGCGGGAACGTCGGCATGGTGCTGCGCACGATCCA
GGGCAAGATCCCGAGCTTCGAAGAGATCAACATGCCGCCGCAGCTGAAGGATATCGCGATGACCCGGCGCGGGCTGGTGA
TCTTCGTCGGCGCGACCTCGTCCGGCAAATCGACCTCGCTGGCTGCGATGATCGGCTACCGCAACCATCACTCGGCCGAT
CACATCATCACCATCGAGGATCCGATCGAGTTCGTGCATGCGCACGACAAGAGCATCGTGACCCAGCGCGAGGTCGGAGT
GGATACCGAAAGCTACGAGATCGCGCTGAAGAACACGCTGCGCCAGGCGCCCGACGTGATCCTGATCGGCGAAATCCGCG
ACCGGGCCACGATGGACTACGGCATCGCGTTCGCCGAGACGGGGCACCTCTGCCTGTCCACACTGCACGCGAACAACACC
AACCAGGCGCTGGATCGGATCATCAACTTCTTCCCCGAGGAGCGCCGGCCACAGTTGCTGATGGATTTGTCGCTTAACCT
GAAGGCCGTGGTGTCGCAGCGCCTGATCCGGACCCAGGACGGCGAAGGGCGGGTGCCGGCGGTCGAGATCATGATCAATA
CACCGCTGATGTCCGACCTGATCCTGAAGGGGGCGGTGCACGAGATGAAGGATCTGATTGCGCGTTCGCGCGAGCAGGGC
ATGCAGACCTTCGACCAGGCGCTGTTCGATCTCTACGAGGCGGGCAAGATCAGTTATCAGGAGGCCATCCGCAACGCGGA
TTCGATGAACGACCTGCGCCTGCGGGTGAAGCTCGACAGCAAGCGGGCGCAGCGCGAGGAGGAGCCCGACGTGATGGACG
ATCTCTCCGGTCTGTCGGTGGCGGACAAGGTCGACGATTTCGTCGCGCGTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W0DQL0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

62.751

89.487

0.562
  pilU Acinetobacter baylyi ADP1

60.286

89.744

0.541
  pilU Vibrio cholerae strain A1552

54.885

89.231

0.49
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.687

85.897

0.367

Multiple sequence alignment