Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QOS47_RS02455 Genome accession   NZ_OX460909
Coordinates   486660..487697 (+) Length   345 a.a.
NCBI ID   WP_045032200.1    Uniprot ID   A0AAW5A668
Organism   Photobacterium phosphoreum strain MIP2473 isolate MIP2473     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 481660..492697
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QOS47_RS02420 - 481664..482062 (+) 399 WP_181317015.1 DUF2884 family protein -
  QOS47_RS02425 hemW 482209..483369 (-) 1161 WP_065193546.1 radical SAM family heme chaperone HemW -
  QOS47_RS02430 - 483369..483962 (-) 594 WP_045032190.1 XTP/dITP diphosphatase -
  QOS47_RS02435 yggU 484135..484431 (-) 297 WP_065195406.1 DUF167 family protein YggU -
  QOS47_RS02440 - 484428..484985 (-) 558 WP_065193544.1 YggT family protein -
  QOS47_RS02445 proC 484995..485813 (-) 819 WP_107197039.1 pyrroline-5-carboxylate reductase -
  QOS47_RS02450 - 485927..486637 (-) 711 WP_065193542.1 YggS family pyridoxal phosphate-dependent enzyme -
  QOS47_RS02455 pilT 486660..487697 (+) 1038 WP_045032200.1 type IV pilus twitching motility protein PilT Machinery gene
  QOS47_RS02460 pilU 487714..488820 (+) 1107 WP_232606883.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QOS47_RS02465 ruvX 489184..489615 (-) 432 WP_045032203.1 Holliday junction resolvase RuvX -
  QOS47_RS02470 - 489612..490175 (-) 564 WP_045032205.1 YqgE/AlgH family protein -
  QOS47_RS02475 gshB 490296..491246 (-) 951 WP_045037803.1 glutathione synthase -
  QOS47_RS02480 rsmE 491264..491995 (-) 732 WP_045032209.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38263.84 Da        Isoelectric Point: 6.3143

>NTDB_id=1158777 QOS47_RS02455 WP_045032200.1 486660..487697(+) (pilT) [Photobacterium phosphoreum strain MIP2473 isolate MIP2473]
MDITELLDFSVKHNASDLHLSAGVPPMIRVDGDVRKLSLPALDHSEVHRLIFDIMNDAQRREYEEKLEVDFSFELPDIGR
FRVNAFHQSRGCSAVLRTIPIHIPTLASLNVPEVFYDIAQLNRGLVLVTGATGSGKSTTIAALVDYINSNYQRHILTIED
PIEFVHTSKSCLINQREVHRDTLSFQNALRSSLREDPDVIVVGELRDQETISLALTAAETGHLVLGTLHTSSAAKTVDRI
IDVFPGSDKSMVRSMLSESLRAVVAQHLLKCTTGGRVACHEVMMATPAIRNLIREDKIAQMYSMIQTGSSLGMQTMEQSV
KMLVAQGMVEAEEGRRILDSSKRGF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1158777 QOS47_RS02455 WP_045032200.1 486660..487697(+) (pilT) [Photobacterium phosphoreum strain MIP2473 isolate MIP2473]
ATGGATATCACCGAATTACTCGATTTTAGTGTAAAGCATAACGCATCAGATCTGCATCTTTCTGCGGGCGTTCCACCAAT
GATAAGAGTTGATGGTGATGTGAGAAAACTCAGTTTACCAGCTTTAGATCATAGCGAAGTCCATCGTCTGATATTTGACA
TTATGAATGATGCTCAGCGTCGTGAATATGAAGAAAAATTAGAAGTCGATTTTTCTTTTGAATTACCCGACATTGGTCGC
TTCAGGGTTAATGCTTTTCATCAGTCGCGTGGCTGTTCAGCTGTGTTGCGAACCATCCCGATTCACATTCCTACGTTAGC
CTCTCTTAATGTGCCAGAGGTTTTTTATGATATTGCTCAGCTCAACCGTGGGCTGGTACTGGTAACAGGTGCAACAGGCT
CTGGTAAATCAACCACGATTGCAGCCTTAGTAGATTATATCAATAGTAATTATCAACGCCATATTTTGACCATTGAAGAT
CCGATTGAATTTGTTCATACCAGTAAAAGCTGTTTGATTAATCAACGTGAAGTACACCGTGACACCTTAAGTTTTCAAAA
TGCACTACGTTCATCGCTACGTGAAGATCCTGATGTGATTGTGGTCGGGGAATTACGCGATCAAGAAACCATTAGTTTAG
CGTTAACCGCTGCAGAAACAGGTCACTTAGTGTTAGGGACATTGCACACCAGCTCAGCCGCTAAAACCGTTGATCGTATT
ATCGATGTTTTTCCCGGTAGTGATAAATCGATGGTGCGCTCAATGTTGTCAGAATCATTACGCGCAGTCGTAGCACAGCA
CTTATTGAAATGTACTACTGGTGGGCGAGTAGCTTGTCATGAAGTAATGATGGCAACCCCTGCTATTCGAAATTTGATCC
GCGAAGATAAAATTGCCCAGATGTATTCAATGATCCAAACCGGTTCATCGCTTGGCATGCAAACTATGGAGCAAAGCGTA
AAAATGCTGGTGGCACAAGGAATGGTCGAAGCTGAAGAAGGGCGTCGTATTCTTGATAGCAGTAAACGTGGTTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

76.522

100

0.765
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

76.522

100

0.765
  pilT Acinetobacter baylyi ADP1

68.129

99.13

0.675
  pilT Acinetobacter baumannii D1279779

68.955

97.101

0.67
  pilT Acinetobacter baumannii strain A118

68.955

97.101

0.67
  pilT Acinetobacter nosocomialis M2

68.657

97.101

0.667
  pilT Pseudomonas stutzeri DSM 10701

68.657

97.101

0.667
  pilT Pseudomonas aeruginosa PAK

68.358

97.101

0.664
  pilT Legionella pneumophila strain Lp02

68.693

95.362

0.655
  pilT Legionella pneumophila strain ERS1305867

68.693

95.362

0.655
  pilT Neisseria meningitidis 8013

62.682

99.42

0.623
  pilT Neisseria gonorrhoeae MS11

62.391

99.42

0.62
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.851

97.101

0.484
  pilU Pseudomonas stutzeri DSM 10701

41.983

99.42

0.417
  pilU Vibrio cholerae strain A1552

41.52

99.13

0.412
  pilU Acinetobacter baylyi ADP1

39.752

93.333

0.371

Multiple sequence alignment