Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QEN43_RS19495 Genome accession   NZ_OX458333
Coordinates   4448900..4449934 (-) Length   344 a.a.
NCBI ID   WP_026609914.1    Uniprot ID   -
Organism   Methylocaldum szegediense isolate Msz(Nor)     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4443900..4454934
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QEN43_RS19465 (MSZNOR_4590) - 4444069..4444299 (-) 231 WP_026609908.1 exodeoxyribonuclease VII small subunit -
  QEN43_RS19470 (MSZNOR_4591) sseA 4444471..4445322 (-) 852 WP_026609909.1 3-mercaptopyruvate sulfurtransferase -
  QEN43_RS19475 (MSZNOR_4592) dapE 4445342..4446472 (-) 1131 WP_026609910.1 succinyl-diaminopimelate desuccinylase -
  QEN43_RS19480 (MSZNOR_4593) - 4446469..4446822 (-) 354 WP_026609911.1 ArsC family reductase -
  QEN43_RS19485 (MSZNOR_4594) - 4446819..4447388 (-) 570 WP_026609912.1 DUF2726 domain-containing protein -
  QEN43_RS19490 (MSZNOR_4595) pilU 4447761..4448882 (-) 1122 WP_026609913.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QEN43_RS19495 (MSZNOR_4596) pilT 4448900..4449934 (-) 1035 WP_026609914.1 type IV pilus twitching motility protein PilT Machinery gene
  QEN43_RS19500 (MSZNOR_4597) - 4450023..4450748 (+) 726 WP_036268090.1 YggS family pyridoxal phosphate-dependent enzyme -
  QEN43_RS19505 (MSZNOR_4598) proC 4450844..4451668 (+) 825 WP_026609915.1 pyrroline-5-carboxylate reductase -
  QEN43_RS19510 (MSZNOR_4599) - 4451671..4452246 (+) 576 WP_026609916.1 YggT family protein -
  QEN43_RS19515 (MSZNOR_4600) - 4452248..4452547 (+) 300 WP_026609917.1 DUF167 family protein -
  QEN43_RS19520 (MSZNOR_4601) - 4452628..4454610 (+) 1983 WP_051331556.1 dynamin family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38057.66 Da        Isoelectric Point: 7.0819

>NTDB_id=1158477 QEN43_RS19495 WP_026609914.1 4448900..4449934(-) (pilT) [Methylocaldum szegediense isolate Msz(Nor)]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDIRRINVPALDHKEVHALIYDIMNDKQRRDYEEFLETDFSFELPGVAR
FRVNAFNQDRGAAAVFRTIPSKVLTLEELGCPKFFQEVTTHPRGLILVTGPTGSGKSTTLAAMIDHINSNQYSHILTIED
PIEFVHTSKKSLINQREVHRDTLGFNEALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTNSAAKTIDRI
IDVFPAAEKGVIRSMLSESLQAVIAQTLLKKNGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGRKDGMQTLDQHL
QELVEKGVVARATARAKAVNKAAF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1158477 QEN43_RS19495 WP_026609914.1 4448900..4449934(-) (pilT) [Methylocaldum szegediense isolate Msz(Nor)]
ATGGATATCGCCGAACTGCTCGCTTTTTCCGTCAAAAACAAGGCTTCCGATTTGCATTTGTCCGCCGGGCTTCCTCCCAT
GATCCGTGTGGATGGCGATATACGCCGGATTAACGTGCCGGCGCTCGACCACAAAGAGGTACACGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGGCGTGATTACGAGGAGTTCCTGGAGACCGACTTTTCTTTCGAGCTCCCTGGAGTCGCGCGC
TTTCGCGTCAACGCGTTCAATCAGGACCGGGGCGCGGCGGCGGTTTTTCGAACCATTCCGTCCAAGGTTTTGACATTGGA
GGAATTGGGTTGCCCAAAATTTTTCCAGGAGGTCACCACGCATCCTCGCGGCCTGATCTTGGTCACCGGGCCCACGGGCT
CAGGCAAATCGACGACTCTCGCCGCGATGATCGACCATATCAATTCGAATCAATACTCCCATATCTTGACGATCGAAGAC
CCCATCGAGTTCGTGCACACCAGCAAGAAATCGCTGATCAACCAACGAGAAGTTCACCGGGATACCTTGGGGTTCAACGA
AGCCTTGCGCTCGGCTCTACGCGAAGACCCCGATGTGATTCTGGTCGGGGAAATGCGCGATCTGGAAACCATTCGTCTGG
CGCTGACCGCCGCGGAAACCGGTCACCTCGTTTTCGGCACCCTGCACACCAACTCAGCAGCCAAAACCATAGACCGCATC
ATTGATGTATTCCCCGCCGCGGAAAAAGGCGTTATTCGGTCGATGCTGTCCGAGTCCTTGCAGGCCGTGATCGCCCAAAC
TCTGCTCAAGAAAAACGGCGGCGGACGCACGGCGGCCTGGGAAATCATGGTGGGCACTCCGGCGATTCGCAATCTCATAC
GCGAAGACAAGGTCGCACAGATGTATTCGGCCATACAAACCGGACGCAAGGACGGGATGCAAACCCTCGACCAGCACTTG
CAAGAATTGGTCGAGAAAGGCGTCGTTGCACGCGCTACCGCGCGCGCCAAGGCGGTTAACAAAGCGGCATTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

80.769

98.256

0.794
  pilT Acinetobacter nosocomialis M2

79.07

100

0.791
  pilT Acinetobacter baumannii D1279779

79.07

100

0.791
  pilT Acinetobacter baumannii strain A118

79.07

100

0.791
  pilT Pseudomonas stutzeri DSM 10701

79.07

100

0.791
  pilT Acinetobacter baylyi ADP1

78.779

100

0.788
  pilT Legionella pneumophila strain Lp02

75.581

100

0.756
  pilT Legionella pneumophila strain ERS1305867

75.581

100

0.756
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.436

97.965

0.68
  pilT Vibrio cholerae strain A1552

69.436

97.965

0.68
  pilT Neisseria meningitidis 8013

67.536

100

0.677
  pilT Neisseria gonorrhoeae MS11

67.246

100

0.674
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

100

0.517
  pilU Acinetobacter baylyi ADP1

40.588

98.837

0.401
  pilU Pseudomonas stutzeri DSM 10701

40.299

97.384

0.392
  pilU Vibrio cholerae strain A1552

41.538

94.477

0.392

Multiple sequence alignment