Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QEN43_RS19490 Genome accession   NZ_OX458333
Coordinates   4447761..4448882 (-) Length   373 a.a.
NCBI ID   WP_026609913.1    Uniprot ID   -
Organism   Methylocaldum szegediense isolate Msz(Nor)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4442761..4453882
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QEN43_RS19460 (MSZNOR_4589) ispA 4443177..4444079 (-) 903 WP_026609907.1 (2E,6E)-farnesyl diphosphate synthase -
  QEN43_RS19465 (MSZNOR_4590) - 4444069..4444299 (-) 231 WP_026609908.1 exodeoxyribonuclease VII small subunit -
  QEN43_RS19470 (MSZNOR_4591) sseA 4444471..4445322 (-) 852 WP_026609909.1 3-mercaptopyruvate sulfurtransferase -
  QEN43_RS19475 (MSZNOR_4592) dapE 4445342..4446472 (-) 1131 WP_026609910.1 succinyl-diaminopimelate desuccinylase -
  QEN43_RS19480 (MSZNOR_4593) - 4446469..4446822 (-) 354 WP_026609911.1 ArsC family reductase -
  QEN43_RS19485 (MSZNOR_4594) - 4446819..4447388 (-) 570 WP_026609912.1 DUF2726 domain-containing protein -
  QEN43_RS19490 (MSZNOR_4595) pilU 4447761..4448882 (-) 1122 WP_026609913.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QEN43_RS19495 (MSZNOR_4596) pilT 4448900..4449934 (-) 1035 WP_026609914.1 type IV pilus twitching motility protein PilT Machinery gene
  QEN43_RS19500 (MSZNOR_4597) - 4450023..4450748 (+) 726 WP_036268090.1 YggS family pyridoxal phosphate-dependent enzyme -
  QEN43_RS19505 (MSZNOR_4598) proC 4450844..4451668 (+) 825 WP_026609915.1 pyrroline-5-carboxylate reductase -
  QEN43_RS19510 (MSZNOR_4599) - 4451671..4452246 (+) 576 WP_026609916.1 YggT family protein -
  QEN43_RS19515 (MSZNOR_4600) - 4452248..4452547 (+) 300 WP_026609917.1 DUF167 family protein -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 42198.54 Da        Isoelectric Point: 6.6911

>NTDB_id=1158476 QEN43_RS19490 WP_026609913.1 4447761..4448882(-) (pilU) [Methylocaldum szegediense isolate Msz(Nor)]
MDFESLLMLMTQKKASDLFIITNKEPCLKIDGVIQPISNTKLTKDQARQIVLGVMNQRQRDEFENTKECNFAISSSGLGR
FRVSAFIQRDTPGMVLRRIETNIPTVEELNLPPILNELAMTKRGLILFVGATGTGKSTSLAAMIKYRNEHSSGHIISIED
PLEYVHPHAGCIITQREVGIDTESYEVALKNTLRQAPDVILIGEIRTRETMQHAITFAETGHLCLSTLHANNANQALDRI
LHFFPEDMHRQLFMDLSLNLRAIIAQQLIRRADGKGRYPAVEILLNTPLASDLIRKGEIHKLKELMKHSREQGMQTFDQA
LYDLYKAGKITYEDALHAADSKNEVRLMIKLGEEGSIEKYSGRHDNMFILDDD

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=1158476 QEN43_RS19490 WP_026609913.1 4447761..4448882(-) (pilU) [Methylocaldum szegediense isolate Msz(Nor)]
GTGGATTTCGAATCCTTACTCATGCTGATGACGCAAAAAAAAGCGTCAGACCTTTTCATCATCACCAACAAAGAACCTTG
CCTGAAGATCGACGGCGTCATTCAACCGATTTCAAATACCAAGCTGACGAAGGATCAGGCGCGGCAAATCGTCCTTGGGG
TGATGAATCAGCGCCAGCGCGACGAGTTCGAGAACACTAAGGAGTGCAATTTCGCCATTTCATCGTCCGGGTTGGGGCGA
TTCCGTGTCAGCGCTTTCATCCAAAGAGACACTCCCGGAATGGTCCTGCGGCGGATCGAGACGAACATTCCGACGGTTGA
AGAGCTCAATCTGCCCCCCATCCTGAATGAATTGGCGATGACCAAACGCGGTCTGATCCTGTTCGTCGGCGCGACCGGCA
CGGGAAAATCCACGTCCCTGGCCGCCATGATCAAATACCGCAACGAGCATTCGAGCGGCCATATCATCTCGATCGAAGAC
CCTCTGGAATACGTTCACCCGCATGCGGGTTGCATCATCACCCAGCGCGAAGTCGGCATTGATACAGAATCGTACGAAGT
GGCGCTCAAAAACACCTTGCGGCAGGCGCCGGACGTCATTCTGATCGGGGAAATCCGAACCCGCGAAACGATGCAGCATG
CGATCACCTTTGCCGAGACCGGCCATCTTTGCCTTAGTACCTTGCACGCCAACAATGCTAACCAGGCACTGGATCGCATT
CTTCACTTTTTTCCCGAGGACATGCACCGGCAGCTGTTCATGGATCTTTCCTTGAATCTTCGAGCCATCATCGCGCAACA
GCTCATTCGCCGAGCCGACGGCAAAGGCCGCTATCCGGCAGTGGAAATTCTCCTCAACACCCCGCTCGCCTCCGACCTCA
TCCGTAAAGGCGAGATCCATAAGCTGAAGGAGCTTATGAAACACTCGCGCGAACAGGGTATGCAGACCTTCGATCAAGCT
TTGTACGATCTTTATAAAGCTGGGAAGATCACCTACGAGGACGCCTTACACGCCGCAGATTCGAAAAACGAAGTGCGGCT
GATGATCAAGCTGGGCGAAGAAGGCAGCATCGAAAAATACTCGGGCCGCCACGACAATATGTTCATCTTAGATGACGATT
GA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

69.209

94.906

0.657
  pilU Acinetobacter baylyi ADP1

66.106

95.71

0.633
  pilU Vibrio cholerae strain A1552

57.429

93.834

0.539
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.529

93.298

0.397
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.95

90.349

0.37
  pilT Vibrio cholerae strain A1552

40.95

90.349

0.37
  pilT Legionella pneumophila strain Lp02

40.233

91.957

0.37
  pilT Legionella pneumophila strain ERS1305867

40.233

91.957

0.37

Multiple sequence alignment