Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QWI75_RS17555 Genome accession   NZ_OX365700
Coordinates   3717053..3718099 (+) Length   348 a.a.
NCBI ID   WP_289270204.1    Uniprot ID   A0AA86T7T6
Organism   Nitrospira tepida strain DNF     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3712053..3723099
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QWI75_RS17540 (DNFV4_03627) - 3713648..3714613 (+) 966 WP_289270198.1 lytic transglycosylase domain-containing protein -
  QWI75_RS17545 (DNFV4_03628) - 3714643..3715440 (-) 798 WP_289270200.1 tetratricopeptide repeat protein -
  QWI75_RS17550 (DNFV4_03629) bioF 3715648..3716826 (+) 1179 WP_289270202.1 8-amino-7-oxononanoate synthase -
  QWI75_RS17555 (DNFV4_03630) pilT 3717053..3718099 (+) 1047 WP_289270204.1 type IV pilus twitching motility protein PilT Machinery gene
  QWI75_RS17560 (DNFV4_03631) pilU 3718159..3719334 (+) 1176 WP_289270206.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QWI75_RS17565 (DNFV4_03632) - 3719358..3719921 (-) 564 WP_289270208.1 c-type cytochrome -
  QWI75_RS17570 (DNFV4_03633) - 3720088..3720918 (-) 831 WP_289270210.1 SUMF1/EgtB/PvdO family nonheme iron enzyme -
  QWI75_RS17580 (DNFV4_03635) - 3721426..3722436 (+) 1011 WP_289270213.1 S1C family serine protease -
  QWI75_RS17585 (DNFV4_03636) - 3722476..3722964 (-) 489 WP_289270214.1 low molecular weight phosphatase family protein -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38289.29 Da        Isoelectric Point: 6.6597

>NTDB_id=1155801 QWI75_RS17555 WP_289270204.1 3717053..3718099(+) (pilT) [Nitrospira tepida strain DNF]
MDISQLLSFGVKEGASDCHLSAGEPPMLRLHGDMKKVDMPPMTQEQVHSLVYDIMSDAQRKSFEEHLECDFSFALGDISR
FRVNVFLQNRGLAAVFRTIPTEILPLEKLGMPPIVRQLCDREKGLILVTGPTGSGKSTTLAGMIDYLNNLYEGHVITIED
PIEFVHKSKKCLVNQRELGVHTKSFANALRSALREDPDIVLVGEMRDLETIQLALTAAETGHLVFATLHTSSAPKTVDRI
IDAFPPNQQAQVRTQLAEALEAVLTQTLVKKKTGGRAAAVEILVATTAIRNLIREQKLHQIPGLMQASQKEGMQTMDMAL
LDLATRGVITKAEAQSRSMNPNLFANAV

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=1155801 QWI75_RS17555 WP_289270204.1 3717053..3718099(+) (pilT) [Nitrospira tepida strain DNF]
ATGGACATTTCCCAACTGCTCAGCTTTGGCGTGAAGGAGGGCGCATCGGACTGTCACCTCAGCGCGGGGGAACCGCCGAT
GTTGCGGCTGCACGGCGACATGAAGAAAGTGGACATGCCTCCCATGACGCAAGAGCAGGTCCATTCCTTGGTCTACGACA
TCATGAGCGACGCCCAACGGAAGAGCTTTGAAGAGCATCTGGAATGTGATTTCTCCTTCGCGCTGGGAGACATCTCCCGC
TTCCGCGTGAACGTGTTCCTGCAGAACCGGGGCTTGGCGGCGGTGTTCCGGACCATCCCGACGGAAATCCTTCCACTTGA
GAAGCTAGGCATGCCCCCCATCGTCCGCCAACTGTGCGATCGTGAAAAGGGCCTCATTCTCGTCACCGGACCGACCGGAT
CAGGCAAGTCCACCACCCTGGCCGGCATGATCGACTACCTGAACAATCTCTACGAAGGGCACGTCATCACGATCGAAGAC
CCGATCGAGTTCGTCCATAAGTCCAAGAAATGCCTGGTCAACCAGCGGGAACTCGGCGTGCACACCAAGTCGTTTGCCAA
CGCGCTCCGATCCGCTCTGCGGGAAGATCCCGATATTGTCCTGGTCGGCGAAATGCGGGATCTCGAAACCATTCAACTGG
CGTTGACCGCCGCGGAAACGGGGCACCTGGTCTTCGCCACCCTGCATACATCCAGCGCGCCCAAGACCGTGGATCGGATC
ATCGACGCCTTCCCGCCCAACCAGCAGGCCCAGGTGCGAACCCAGCTCGCGGAGGCGCTGGAGGCCGTGCTCACGCAGAC
GCTCGTCAAGAAAAAGACGGGCGGCCGCGCCGCCGCCGTGGAGATTCTGGTCGCCACCACTGCGATCCGCAACCTCATCC
GAGAACAGAAGCTCCACCAAATTCCCGGCTTGATGCAGGCCAGCCAGAAGGAAGGGATGCAGACCATGGATATGGCCCTG
CTCGATCTGGCCACGCGGGGCGTGATCACCAAGGCCGAGGCCCAATCGCGCAGCATGAACCCGAACCTGTTTGCCAACGC
CGTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-261)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

60.174

98.851

0.595
  pilT Pseudomonas stutzeri DSM 10701

59.884

98.851

0.592
  pilT Acinetobacter baylyi ADP1

58.721

98.851

0.58
  pilT Legionella pneumophila strain ERS1305867

58.43

98.851

0.578
  pilT Legionella pneumophila strain Lp02

58.43

98.851

0.578
  pilT Acinetobacter nosocomialis M2

58.14

98.851

0.575
  pilT Acinetobacter baumannii D1279779

58.14

98.851

0.575
  pilT Acinetobacter baumannii strain A118

58.14

98.851

0.575
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

59.05

96.839

0.572
  pilT Vibrio cholerae strain A1552

59.05

96.839

0.572
  pilT Neisseria meningitidis 8013

57.771

97.989

0.566
  pilT Neisseria gonorrhoeae MS11

57.478

97.989

0.563
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.578

99.425

0.503
  pilU Pseudomonas stutzeri DSM 10701

42.687

96.264

0.411
  pilU Acinetobacter baylyi ADP1

40.407

98.851

0.399
  pilU Vibrio cholerae strain A1552

39.403

96.264

0.379

Multiple sequence alignment