Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QWI75_RS17560 Genome accession   NZ_OX365700
Coordinates   3718159..3719334 (+) Length   391 a.a.
NCBI ID   WP_289270206.1    Uniprot ID   A0AA86TA45
Organism   Nitrospira tepida strain DNF     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3713159..3724334
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QWI75_RS17540 (DNFV4_03627) - 3713648..3714613 (+) 966 WP_289270198.1 lytic transglycosylase domain-containing protein -
  QWI75_RS17545 (DNFV4_03628) - 3714643..3715440 (-) 798 WP_289270200.1 tetratricopeptide repeat protein -
  QWI75_RS17550 (DNFV4_03629) bioF 3715648..3716826 (+) 1179 WP_289270202.1 8-amino-7-oxononanoate synthase -
  QWI75_RS17555 (DNFV4_03630) pilT 3717053..3718099 (+) 1047 WP_289270204.1 type IV pilus twitching motility protein PilT Machinery gene
  QWI75_RS17560 (DNFV4_03631) pilU 3718159..3719334 (+) 1176 WP_289270206.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QWI75_RS17565 (DNFV4_03632) - 3719358..3719921 (-) 564 WP_289270208.1 c-type cytochrome -
  QWI75_RS17570 (DNFV4_03633) - 3720088..3720918 (-) 831 WP_289270210.1 SUMF1/EgtB/PvdO family nonheme iron enzyme -
  QWI75_RS17580 (DNFV4_03635) - 3721426..3722436 (+) 1011 WP_289270213.1 S1C family serine protease -
  QWI75_RS17585 (DNFV4_03636) - 3722476..3722964 (-) 489 WP_289270214.1 low molecular weight phosphatase family protein -
  QWI75_RS17590 (DNFV4_03637) ttcA 3723035..3723931 (+) 897 WP_289270216.1 tRNA 2-thiocytidine(32) synthetase TtcA -

Sequence

Protein


Download         Length: 391 a.a.        Molecular weight: 44158.93 Da        Isoelectric Point: 7.0685

>NTDB_id=1155802 QWI75_RS17560 WP_289270206.1 3718159..3719334(+) (pilU) [Nitrospira tepida strain DNF]
MEIRELLKTMVDREGSDLYLTVDAPPIYRIHGMTHPTSDPPFTNEQLEALALALMRGPQRSEFEEKMEMNLALYYKDLGR
FRVNVFRQRGNVGLVFRHIKAEIQSVEQLGLPPIIKDLAMTKRGLILVVGATGSGKSTTLAAMIDHRNSVSPGHIISVED
PIEFVHPHKKCLITQREVGFDTLSFHNALKNTLRQAPDVILIGEIRDTETMEAAITFAETGHLCLGTLHSNNANQAIERI
MNFFPVERHQQIYLQLSLNLRAILSQRLVPSLDGRRAPAIEIMLDTPRIKDLIKKAEIDTLKEAMEQGVEEGCQTFDYVL
FTLYKEGKISLEQALINADSANNLRLKIKLEGVRSGDEQLSKLLEGDNKDKTRDRKATFQIVGNEPTLRRK

Nucleotide


Download         Length: 1176 bp        

>NTDB_id=1155802 QWI75_RS17560 WP_289270206.1 3718159..3719334(+) (pilU) [Nitrospira tepida strain DNF]
ATGGAAATTCGCGAGCTGCTCAAGACCATGGTCGACCGCGAAGGGTCGGACCTGTACCTCACCGTGGATGCGCCGCCCAT
CTACCGCATCCATGGGATGACGCACCCCACGTCGGACCCTCCCTTCACCAACGAACAATTGGAGGCCCTGGCGCTCGCCT
TGATGCGCGGACCGCAGCGAAGCGAGTTCGAGGAGAAGATGGAGATGAATCTGGCCCTCTATTACAAAGACCTGGGCCGT
TTCCGCGTGAACGTCTTTCGCCAGCGGGGCAACGTGGGCCTCGTGTTTCGGCACATCAAGGCGGAGATTCAGAGCGTGGA
GCAGCTCGGACTGCCCCCGATCATCAAGGACCTCGCCATGACCAAGCGCGGGTTGATCCTCGTGGTCGGCGCCACAGGGT
CCGGAAAATCTACCACCCTGGCGGCCATGATCGACCACCGCAACTCGGTCTCGCCCGGACATATCATCTCGGTTGAGGAT
CCGATCGAGTTCGTGCACCCGCACAAGAAATGCCTCATCACCCAACGTGAAGTCGGCTTCGACACCTTGTCCTTCCACAA
CGCGCTCAAGAACACCCTGCGGCAGGCGCCGGACGTGATCCTCATCGGCGAAATCCGGGACACGGAAACCATGGAAGCCG
CCATCACGTTCGCGGAAACCGGCCATCTCTGCCTGGGCACGCTGCACTCCAACAACGCCAACCAGGCCATCGAACGCATC
ATGAACTTCTTTCCGGTGGAGCGACACCAGCAGATCTACCTGCAACTGTCCTTGAACCTGCGCGCCATCCTGTCCCAGCG
CCTCGTCCCCTCTCTGGACGGACGGCGGGCGCCGGCGATCGAGATCATGCTGGATACCCCCCGCATCAAGGACCTGATCA
AGAAGGCCGAGATCGACACGCTGAAGGAAGCGATGGAACAGGGCGTCGAAGAAGGGTGCCAGACTTTCGATTACGTCCTG
TTTACCCTGTACAAAGAAGGAAAGATCAGCCTGGAGCAGGCTTTGATCAACGCCGACAGCGCCAATAACCTCCGCCTCAA
GATCAAGCTGGAGGGGGTGCGGAGCGGAGATGAGCAGCTCTCGAAACTGTTGGAGGGAGACAACAAAGACAAAACCCGAG
ACCGCAAGGCCACCTTCCAGATTGTCGGAAACGAACCTACCCTCCGGAGGAAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.861

93.862

0.496
  pilU Acinetobacter baylyi ADP1

50.131

97.442

0.488
  pilU Vibrio cholerae strain A1552

50.273

93.606

0.471
  pilT Legionella pneumophila strain Lp02

45.509

85.422

0.389
  pilT Legionella pneumophila strain ERS1305867

45.509

85.422

0.389
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.643

85.934

0.384
  pilT Acinetobacter baylyi ADP1

43.824

86.957

0.381
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.544

85.166

0.371
  pilT Vibrio cholerae strain A1552

43.544

85.166

0.371
  pilT Pseudomonas aeruginosa PAK

43.413

85.422

0.371
  pilT Acinetobacter baumannii strain A118

43.114

85.422

0.368
  pilT Acinetobacter nosocomialis M2

43.114

85.422

0.368
  pilT Acinetobacter baumannii D1279779

43.114

85.422

0.368
  pilT Pseudomonas stutzeri DSM 10701

41.86

87.98

0.368

Multiple sequence alignment