Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ALT831_RS15035 Genome accession   NZ_OX359241
Coordinates   3413915..3414958 (-) Length   347 a.a.
NCBI ID   WP_014950464.1    Uniprot ID   -
Organism   Alteromonas macleodii isolate Alteromonas macleodii MIT1002     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3408915..3419958
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ALT831_RS15005 (MIT1002_03054) - 3409350..3410078 (+) 729 WP_014980069.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ALT831_RS15010 (MIT1002_03055) gshB 3410217..3411170 (+) 954 WP_049586041.1 glutathione synthase -
  ALT831_RS15015 (MIT1002_03056) - 3411299..3411865 (+) 567 WP_049586040.1 YqgE/AlgH family protein -
  ALT831_RS15020 (MIT1002_03057) ruvX 3411858..3412289 (+) 432 WP_014980071.1 Holliday junction resolvase RuvX -
  ALT831_RS15025 (MIT1002_03058) - 3412336..3412653 (-) 318 WP_049586039.1 DUF2007 domain-containing protein -
  ALT831_RS15030 (MIT1002_03059) pilU 3412794..3413906 (-) 1113 WP_014977241.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ALT831_RS15035 (MIT1002_03060) pilT 3413915..3414958 (-) 1044 WP_014950464.1 type IV pilus twitching motility protein PilT Machinery gene
  ALT831_RS15040 (MIT1002_03061) - 3414986..3415672 (+) 687 WP_049586038.1 YggS family pyridoxal phosphate-dependent enzyme -
  ALT831_RS15045 (MIT1002_03062) proC 3415743..3416564 (+) 822 WP_049586037.1 pyrroline-5-carboxylate reductase -
  ALT831_RS15050 (MIT1002_03063) - 3416585..3417121 (+) 537 WP_014950467.1 YggT family protein -
  ALT831_RS15055 (MIT1002_03064) - 3417218..3417691 (+) 474 WP_049586036.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38380.09 Da        Isoelectric Point: 6.5677

>NTDB_id=1155768 ALT831_RS15035 WP_014950464.1 3413915..3414958(-) (pilT) [Alteromonas macleodii isolate Alteromonas macleodii MIT1002]
MDITELLAFSVKNNASDLHLSAGLPPIIRVDGEMRKLNVPALDHKQVHGLVYEIMNDMQRKEYEENLETDFSFEVNGLSR
FRVNAFVQNRGAAAVLRTIPSKVLTLDDLGAPEIFKEIINQPTGIVLVTGATGSGKSTTLAAMVDHINTHKREHILTIED
PIEFVHDNKLSLVNQREVHRDTHSFNNALRSALREDPDVILVGELRDLETIRLAISAAETGHLVFGTLHTNSAPKTIDRI
IDVFPAEEKAMVRSMLSESLRAVISQTLLKKVGGGRVAAHEIMVGIPAIRNLIREDKVPQMYSVIQTGQAHGMQTMDQCL
QRLVAMGVITKQDAAAKSVDKQSMNSF

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1155768 ALT831_RS15035 WP_014950464.1 3413915..3414958(-) (pilT) [Alteromonas macleodii isolate Alteromonas macleodii MIT1002]
GTGGATATTACCGAACTTTTGGCTTTCAGTGTTAAGAATAACGCCTCAGATTTACACTTATCAGCGGGCCTACCACCAAT
AATTCGTGTAGATGGCGAAATGCGTAAACTGAATGTGCCAGCCCTCGATCACAAGCAAGTACATGGTCTCGTTTATGAGA
TTATGAATGACATGCAGCGCAAAGAATACGAAGAAAACCTGGAAACCGATTTTTCTTTCGAAGTTAATGGCTTGTCGCGC
TTTCGTGTTAACGCGTTTGTGCAAAACCGAGGCGCCGCCGCAGTATTACGTACTATCCCCAGTAAAGTATTAACCTTAGA
CGACCTTGGCGCGCCTGAAATTTTCAAGGAAATTATAAATCAGCCTACCGGCATTGTACTTGTGACAGGTGCCACCGGCT
CGGGTAAAAGTACCACGCTTGCGGCCATGGTCGACCATATCAACACCCATAAGCGCGAGCACATTCTTACCATTGAAGAC
CCTATCGAATTCGTGCACGACAACAAGCTTAGCTTGGTAAACCAGCGGGAAGTACACCGCGATACGCACTCGTTTAATAA
CGCACTGCGCTCGGCGCTTCGTGAAGACCCAGACGTAATTCTAGTCGGTGAGCTACGTGACTTAGAAACCATACGCCTTG
CCATTTCAGCTGCCGAAACCGGCCATTTAGTATTTGGCACTCTTCACACCAACTCTGCACCAAAAACCATTGACCGTATT
ATCGATGTTTTCCCGGCAGAAGAAAAAGCCATGGTGCGTTCTATGTTGTCGGAATCATTGCGTGCGGTTATTTCACAGAC
ACTGCTTAAGAAAGTAGGCGGGGGCCGAGTTGCGGCTCATGAAATCATGGTGGGGATCCCCGCTATTCGTAACCTTATTC
GAGAAGATAAAGTGCCGCAAATGTACTCGGTTATTCAAACGGGTCAGGCTCACGGTATGCAAACCATGGATCAGTGTCTT
CAGCGCTTAGTGGCAATGGGGGTTATTACCAAACAAGACGCCGCAGCAAAGTCAGTTGATAAACAGTCGATGAACAGTTT
CTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

75.872

99.135

0.752
  pilT Acinetobacter baumannii strain A118

75.872

99.135

0.752
  pilT Acinetobacter nosocomialis M2

75.581

99.135

0.749
  pilT Pseudomonas stutzeri DSM 10701

74.783

99.424

0.744
  pilT Acinetobacter baylyi ADP1

73.837

99.135

0.732
  pilT Pseudomonas aeruginosa PAK

74.852

97.406

0.729
  pilT Legionella pneumophila strain Lp02

71.848

98.271

0.706
  pilT Legionella pneumophila strain ERS1305867

71.848

98.271

0.706
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.7

97.118

0.706
  pilT Vibrio cholerae strain A1552

72.7

97.118

0.706
  pilT Neisseria meningitidis 8013

65.029

99.712

0.648
  pilT Neisseria gonorrhoeae MS11

64.74

99.712

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.895

98.847

0.513
  pilU Pseudomonas stutzeri DSM 10701

41.791

96.542

0.403
  pilU Vibrio cholerae strain A1552

41.791

96.542

0.403
  pilU Acinetobacter baylyi ADP1

41.42

97.406

0.403

Multiple sequence alignment