Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ALT831_RS15030 Genome accession   NZ_OX359241
Coordinates   3412794..3413906 (-) Length   370 a.a.
NCBI ID   WP_014977241.1    Uniprot ID   A0AB33A1W2
Organism   Alteromonas macleodii isolate Alteromonas macleodii MIT1002     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3407794..3418906
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ALT831_RS15005 (MIT1002_03054) - 3409350..3410078 (+) 729 WP_014980069.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ALT831_RS15010 (MIT1002_03055) gshB 3410217..3411170 (+) 954 WP_049586041.1 glutathione synthase -
  ALT831_RS15015 (MIT1002_03056) - 3411299..3411865 (+) 567 WP_049586040.1 YqgE/AlgH family protein -
  ALT831_RS15020 (MIT1002_03057) ruvX 3411858..3412289 (+) 432 WP_014980071.1 Holliday junction resolvase RuvX -
  ALT831_RS15025 (MIT1002_03058) - 3412336..3412653 (-) 318 WP_049586039.1 DUF2007 domain-containing protein -
  ALT831_RS15030 (MIT1002_03059) pilU 3412794..3413906 (-) 1113 WP_014977241.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ALT831_RS15035 (MIT1002_03060) pilT 3413915..3414958 (-) 1044 WP_014950464.1 type IV pilus twitching motility protein PilT Machinery gene
  ALT831_RS15040 (MIT1002_03061) - 3414986..3415672 (+) 687 WP_049586038.1 YggS family pyridoxal phosphate-dependent enzyme -
  ALT831_RS15045 (MIT1002_03062) proC 3415743..3416564 (+) 822 WP_049586037.1 pyrroline-5-carboxylate reductase -
  ALT831_RS15050 (MIT1002_03063) - 3416585..3417121 (+) 537 WP_014950467.1 YggT family protein -
  ALT831_RS15055 (MIT1002_03064) - 3417218..3417691 (+) 474 WP_049586036.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40781.69 Da        Isoelectric Point: 6.0487

>NTDB_id=1155767 ALT831_RS15030 WP_014977241.1 3412794..3413906(-) (pilU) [Alteromonas macleodii isolate Alteromonas macleodii MIT1002]
MLDSLLQKMVDTNASDLFVTAGFPVSAKINGKLTPVTESATSEEASLALVHEAMTEKQQNEFHTTKECNFAIVREGIGRF
RCSAFWQRDQAGMVIRRIVTQIPKADDLGLPRVLKEIIMAKRGLVLFVGGTGTGKSTSLAALIGHRNENSHGHILTIEDP
IEFVHEHKNCVITQREVGIDTQSFDDALKSSLRQAPDVILIGEIRSMETMEYAMSFADTGHLCVATLHANNANQAIERIM
HLAPKDQHDKLRFDLSLNIRAIVAQQLVPTPDGKGRVAAIEILLNSPLVRDLIQRNEIGALKEAMKKGKEQGMQSFDMAL
YDLYKAGKIDMDQALHHADSPNDLRLMIKLDTSDGSSLGTLSNVSIDMDD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=1155767 ALT831_RS15030 WP_014977241.1 3412794..3413906(-) (pilU) [Alteromonas macleodii isolate Alteromonas macleodii MIT1002]
ATGTTAGATTCACTACTACAAAAAATGGTCGATACCAACGCATCGGATTTATTCGTCACTGCGGGGTTTCCGGTAAGTGC
CAAAATTAACGGCAAACTTACGCCAGTGACGGAAAGCGCGACCTCGGAAGAGGCATCGCTGGCGCTGGTTCACGAAGCGA
TGACTGAAAAACAGCAGAATGAGTTTCACACGACCAAAGAATGTAATTTCGCCATAGTACGAGAGGGCATTGGTCGTTTC
CGCTGCTCTGCATTTTGGCAGCGTGACCAAGCGGGCATGGTGATTCGCCGCATCGTTACTCAAATTCCGAAAGCTGATGA
TTTGGGTCTGCCGCGAGTATTAAAAGAAATTATTATGGCTAAACGCGGTCTTGTTCTATTTGTAGGTGGAACAGGCACGG
GTAAGTCCACGTCACTGGCTGCGCTTATTGGGCATAGAAATGAAAATTCGCACGGGCATATACTGACTATTGAAGACCCC
ATTGAATTTGTGCACGAGCATAAAAACTGTGTTATTACCCAGCGCGAGGTGGGCATAGATACTCAGTCTTTCGACGACGC
CCTTAAAAGTTCGCTACGTCAGGCACCTGATGTCATTCTTATTGGTGAAATTCGTTCAATGGAAACCATGGAATATGCCA
TGTCGTTTGCTGATACCGGGCATTTGTGTGTGGCTACGCTGCACGCTAACAACGCGAACCAAGCTATTGAACGTATTATG
CATTTAGCGCCGAAAGATCAGCACGATAAGTTGCGCTTTGACTTAAGCTTAAACATCCGCGCCATTGTTGCGCAGCAACT
AGTACCTACGCCAGACGGCAAGGGTAGGGTTGCGGCAATAGAAATATTGCTTAATTCGCCGCTAGTGCGCGACCTAATAC
AGCGCAATGAAATAGGCGCGTTAAAAGAAGCAATGAAAAAAGGCAAAGAGCAGGGCATGCAGAGCTTTGATATGGCCCTG
TATGATTTGTACAAAGCGGGTAAGATTGATATGGACCAAGCGCTGCACCACGCCGACTCGCCTAATGACCTGCGCTTAAT
GATCAAGCTTGATACCAGCGACGGTTCTAGCTTAGGTACACTGTCAAATGTATCTATTGATATGGATGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(5-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.377

100

0.605
  pilU Acinetobacter baylyi ADP1

59.143

94.595

0.559
  pilU Vibrio cholerae strain A1552

56.25

99.459

0.559
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.647

91.892

0.392
  pilT Acinetobacter nosocomialis M2

39.412

91.892

0.362
  pilT Acinetobacter baumannii D1279779

39.412

91.892

0.362
  pilT Acinetobacter baumannii strain A118

39.412

91.892

0.362

Multiple sequence alignment