Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OM978_RS13150 Genome accession   NZ_OX352320
Coordinates   2804683..2805747 (-) Length   354 a.a.
NCBI ID   WP_264342648.1    Uniprot ID   -
Organism   Rheinheimera sp. MM224     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2799683..2810747
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OM978_RS13130 (JAMGFMIE_02645) - 2801777..2802073 (+) 297 WP_264342645.1 nucleotidyltransferase domain-containing protein -
  OM978_RS13135 (JAMGFMIE_02646) - 2802070..2802567 (+) 498 WP_264342646.1 hypothetical protein -
  OM978_RS13140 (JAMGFMIE_02647) - 2802600..2803505 (+) 906 WP_264346946.1 FRG domain-containing protein -
  OM978_RS13145 (JAMGFMIE_02648) pilU 2803559..2804677 (-) 1119 WP_264342647.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OM978_RS13150 (JAMGFMIE_02649) pilT 2804683..2805747 (-) 1065 WP_264342648.1 type IV pilus twitching motility protein PilT Machinery gene
  OM978_RS13155 (JAMGFMIE_02650) - 2805790..2806479 (+) 690 WP_264342649.1 YggS family pyridoxal phosphate-dependent enzyme -
  OM978_RS13160 (JAMGFMIE_02651) proC 2806525..2807340 (+) 816 WP_264342650.1 pyrroline-5-carboxylate reductase -
  OM978_RS13165 (JAMGFMIE_02652) - 2807350..2807892 (+) 543 WP_264342651.1 YggT family protein -
  OM978_RS13170 (JAMGFMIE_02653) - 2807893..2808183 (+) 291 WP_233009822.1 DUF167 family protein -
  OM978_RS13175 (JAMGFMIE_02654) - 2808183..2808608 (+) 426 WP_264342652.1 DUF4426 domain-containing protein -
  OM978_RS13180 (JAMGFMIE_02655) rdgB 2808663..2809277 (+) 615 WP_264342653.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  OM978_RS13185 (JAMGFMIE_02656) hemW 2809277..2810440 (+) 1164 WP_264342654.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 39122.75 Da        Isoelectric Point: 6.9821

>NTDB_id=1155311 OM978_RS13150 WP_264342648.1 2804683..2805747(-) (pilT) [Rheinheimera sp. MM224]
MDITELLAFSVQHKASDLHLSAGVPPLIRVDGDVRRLNIPALDHKAVHSLVYDIMNDRQRKEYEEKLESDFSFEVPGLAR
FRVNAFVQNRGAAAVFRTIPSDVLSLEDLNAPEVFRSISDNPRGLVLVTGPTGSGKSTTLAAMIDYINTTKNHHILTIED
PIEFVHQNKLSLINQREVHRDTHSFSNALRSALREDPDVILVGELRDLETIRLAMTAAETGHLVFGTLHTTSAPKTIDRI
IDVFPGEEKAMVRSMLSESLRAVISQTLLKKNGGGRVAAHEIMLGVPAIRNLIREDKIAQMYSVIQTGASHGMQTMDQCL
INLVNRGTINQKDALAKAQDKNSFNSMGSMGRMG

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=1155311 OM978_RS13150 WP_264342648.1 2804683..2805747(-) (pilT) [Rheinheimera sp. MM224]
ATGGATATTACCGAATTACTCGCCTTTAGTGTGCAACATAAAGCCTCGGATCTGCATTTATCGGCCGGTGTACCGCCACT
TATCCGGGTGGATGGGGATGTGCGACGTTTAAATATTCCGGCTTTAGACCATAAAGCAGTGCACTCTTTGGTGTACGACA
TTATGAATGACCGTCAGCGTAAAGAATACGAAGAAAAGCTCGAATCCGATTTTTCTTTTGAAGTGCCGGGTTTAGCGCGT
TTTCGTGTCAATGCGTTTGTGCAAAACCGCGGTGCTGCTGCTGTATTTCGTACCATTCCAAGTGATGTATTAAGTCTTGA
AGATTTAAATGCACCTGAAGTATTTCGAAGTATTTCTGACAATCCTCGTGGCCTGGTGCTGGTGACTGGTCCTACAGGCT
CAGGTAAATCTACTACTTTGGCGGCGATGATTGACTACATCAACACCACCAAAAACCATCATATTCTGACGATAGAAGAC
CCTATTGAATTTGTGCACCAGAATAAGCTGAGTTTAATTAACCAGCGTGAAGTGCACCGTGATACTCATAGTTTCAGCAA
TGCCTTGCGCTCAGCCTTGCGTGAAGACCCTGATGTAATTTTGGTGGGCGAGTTGCGGGATTTAGAAACTATTCGTCTGG
CAATGACAGCAGCTGAAACGGGTCATTTGGTGTTTGGCACTTTGCACACCACCTCGGCACCAAAAACCATAGACAGGATT
ATTGACGTATTCCCGGGTGAAGAAAAAGCCATGGTGCGTTCTATGTTGTCTGAGTCGCTCAGAGCTGTTATTTCACAAAC
GTTGTTGAAGAAAAATGGCGGTGGCAGGGTAGCAGCACATGAAATTATGCTGGGGGTTCCGGCCATTCGAAACTTAATTC
GTGAAGATAAAATTGCCCAGATGTATTCGGTGATCCAAACCGGTGCCTCTCATGGTATGCAAACTATGGATCAATGCCTG
ATTAATCTTGTAAACCGAGGAACCATTAATCAAAAAGATGCGTTGGCTAAAGCCCAGGATAAAAACTCCTTTAACAGCAT
GGGCTCTATGGGCCGGATGGGGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

77.616

97.175

0.754
  pilT Acinetobacter nosocomialis M2

77.326

97.175

0.751
  pilT Acinetobacter baumannii D1279779

77.326

97.175

0.751
  pilT Acinetobacter baumannii strain A118

77.326

97.175

0.751
  pilT Pseudomonas aeruginosa PAK

77.035

97.175

0.749
  pilT Acinetobacter baylyi ADP1

77.035

97.175

0.749
  pilT Legionella pneumophila strain Lp02

72.965

97.175

0.709
  pilT Legionella pneumophila strain ERS1305867

72.965

97.175

0.709
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.566

95.763

0.695
  pilT Vibrio cholerae strain A1552

72.566

95.763

0.695
  pilT Neisseria meningitidis 8013

67.919

97.74

0.664
  pilT Neisseria gonorrhoeae MS11

67.63

97.74

0.661
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

54.493

97.458

0.531
  pilU Vibrio cholerae strain A1552

42.529

98.305

0.418
  pilU Pseudomonas stutzeri DSM 10701

40.909

99.435

0.407
  pilU Acinetobacter baylyi ADP1

39.306

97.74

0.384

Multiple sequence alignment