Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   OM978_RS13145 Genome accession   NZ_OX352320
Coordinates   2803559..2804677 (-) Length   372 a.a.
NCBI ID   WP_264342647.1    Uniprot ID   -
Organism   Rheinheimera sp. MM224     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2798559..2809677
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OM978_RS13125 (JAMGFMIE_02644) - 2799287..2801662 (+) 2376 WP_264342644.1 EAL domain-containing protein -
  OM978_RS13130 (JAMGFMIE_02645) - 2801777..2802073 (+) 297 WP_264342645.1 nucleotidyltransferase domain-containing protein -
  OM978_RS13135 (JAMGFMIE_02646) - 2802070..2802567 (+) 498 WP_264342646.1 hypothetical protein -
  OM978_RS13140 (JAMGFMIE_02647) - 2802600..2803505 (+) 906 WP_264346946.1 FRG domain-containing protein -
  OM978_RS13145 (JAMGFMIE_02648) pilU 2803559..2804677 (-) 1119 WP_264342647.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OM978_RS13150 (JAMGFMIE_02649) pilT 2804683..2805747 (-) 1065 WP_264342648.1 type IV pilus twitching motility protein PilT Machinery gene
  OM978_RS13155 (JAMGFMIE_02650) - 2805790..2806479 (+) 690 WP_264342649.1 YggS family pyridoxal phosphate-dependent enzyme -
  OM978_RS13160 (JAMGFMIE_02651) proC 2806525..2807340 (+) 816 WP_264342650.1 pyrroline-5-carboxylate reductase -
  OM978_RS13165 (JAMGFMIE_02652) - 2807350..2807892 (+) 543 WP_264342651.1 YggT family protein -
  OM978_RS13170 (JAMGFMIE_02653) - 2807893..2808183 (+) 291 WP_233009822.1 DUF167 family protein -
  OM978_RS13175 (JAMGFMIE_02654) - 2808183..2808608 (+) 426 WP_264342652.1 DUF4426 domain-containing protein -
  OM978_RS13180 (JAMGFMIE_02655) rdgB 2808663..2809277 (+) 615 WP_264342653.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 40406.48 Da        Isoelectric Point: 6.2384

>NTDB_id=1155310 OM978_RS13145 WP_264342647.1 2803559..2804677(-) (pilU) [Rheinheimera sp. MM224]
MTLVDYLHKMVAAGASDLFITAGLAASAKINGEMQPIAEQVLDSEQSLALVLSAMTPKQQAQFQQDKECNFAIANDAGRF
RVSAFWQRDQAGMVVRRIVTTIPKADDLGLPPVLKDIIMSKRGLVLFVGGTGAGKSTSLAALLGYRNQNARGHILTIEDP
IEFVHDHQKSLVTQREVGIDTESFEAALKSSLRQAPDVILIGEIRSQDTMEYALSFAETGHLCIATLHANNANQAIDRIM
HLVPKEKHGKLLFDLSLNLRAIVAQQLVPTTDGKKRVAAIEVLLNSPLIADLIKRGEIGEIKAVMGKSRELGMQTFDQAL
YELYKAAQISYADALHYADSPNDLRLMMKLQEGTTGSGLLSGVTVAGLTDND

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=1155310 OM978_RS13145 WP_264342647.1 2803559..2804677(-) (pilU) [Rheinheimera sp. MM224]
ATGACTTTGGTTGATTATTTACACAAAATGGTGGCTGCTGGTGCTTCGGATTTATTTATTACGGCTGGCCTTGCGGCATC
CGCCAAAATTAACGGCGAGATGCAGCCGATAGCCGAACAAGTGCTGGACTCTGAGCAATCACTGGCGTTGGTGTTATCTG
CTATGACGCCAAAACAACAAGCCCAGTTTCAGCAGGATAAAGAATGTAACTTCGCTATTGCCAATGACGCCGGGCGTTTT
CGGGTATCAGCCTTCTGGCAGCGGGATCAGGCTGGCATGGTGGTGCGCCGTATCGTTACTACTATTCCAAAAGCAGACGA
TTTAGGCCTGCCGCCCGTATTAAAAGACATCATTATGTCTAAACGGGGGCTGGTGTTATTTGTGGGCGGCACAGGCGCGG
GTAAATCTACCTCGCTTGCGGCATTACTTGGCTATCGCAACCAAAATGCCCGTGGTCATATTTTAACTATCGAAGATCCG
ATTGAATTTGTGCACGATCACCAAAAAAGCCTGGTGACTCAGCGTGAAGTGGGGATAGATACCGAATCCTTTGAAGCGGC
GCTGAAAAGCTCGTTACGTCAGGCACCTGATGTGATTTTAATAGGCGAAATCCGCAGCCAGGACACCATGGAATATGCTC
TGTCTTTTGCTGAAACCGGTCACTTATGTATAGCTACATTACATGCCAACAATGCCAACCAGGCCATTGATCGTATTATG
CATTTGGTGCCAAAAGAAAAACACGGCAAGCTGTTGTTTGATTTATCACTAAATTTACGTGCCATAGTTGCTCAACAACT
GGTACCAACTACAGATGGTAAAAAGCGGGTGGCTGCTATTGAAGTACTGTTAAATTCACCACTAATTGCCGACCTGATTA
AACGTGGCGAGATAGGTGAAATTAAAGCTGTGATGGGCAAATCCCGCGAGCTGGGTATGCAAACCTTTGATCAGGCGTTG
TACGAATTATACAAAGCTGCTCAAATCAGCTACGCCGACGCGCTGCATTATGCCGACTCACCAAACGATTTACGCTTGAT
GATGAAACTGCAGGAAGGCACAACAGGCTCAGGTTTATTGTCTGGTGTGACAGTGGCGGGTTTAACGGATAATGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.574

100

0.602
  pilU Vibrio cholerae strain A1552

59.016

98.387

0.581
  pilU Acinetobacter baylyi ADP1

59.887

95.161

0.57
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.504

96.505

0.401
  pilT Legionella pneumophila strain Lp02

41.867

89.247

0.374
  pilT Legionella pneumophila strain ERS1305867

41.867

89.247

0.374
  pilT Pseudomonas stutzeri DSM 10701

40

92.742

0.371
  pilT Pseudomonas aeruginosa PAK

39.71

92.742

0.368
  pilT Acinetobacter nosocomialis M2

40.058

91.935

0.368
  pilT Acinetobacter baumannii D1279779

40.058

91.935

0.368
  pilT Acinetobacter baumannii strain A118

40.058

91.935

0.368
  pilT Acinetobacter baylyi ADP1

39.181

91.935

0.36

Multiple sequence alignment