Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   Q0V31_RS07655 Genome accession   NZ_OX044366
Coordinates   1611755..1612789 (+) Length   344 a.a.
NCBI ID   WP_298186429.1    Uniprot ID   -
Organism   MAG: uncultured Pseudomonas sp. isolate AG_MB4     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1606755..1617789
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q0V31_RS07630 - 1607029..1609011 (-) 1983 WP_298186416.1 dynamin-like GTPase family protein -
  Q0V31_RS07635 - 1609241..1609537 (-) 297 WP_298186419.1 DUF167 family protein -
  Q0V31_RS07640 - 1609551..1610141 (-) 591 WP_298186423.1 YggT family protein -
  Q0V31_RS07645 proC 1610151..1610969 (-) 819 WP_298186425.1 pyrroline-5-carboxylate reductase -
  Q0V31_RS07650 - 1611005..1611691 (-) 687 WP_298186427.1 YggS family pyridoxal phosphate-dependent enzyme -
  Q0V31_RS07655 pilT 1611755..1612789 (+) 1035 WP_298186429.1 type IV pilus twitching motility protein PilT Machinery gene
  Q0V31_RS07660 pilU 1612896..1614041 (+) 1146 WP_298186431.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Q0V31_RS07665 - 1614143..1614544 (+) 402 WP_298186432.1 NINE protein -
  Q0V31_RS07670 - 1614653..1615924 (-) 1272 WP_298186434.1 dihydroorotase -
  Q0V31_RS07675 - 1615924..1616934 (-) 1011 WP_298186437.1 aspartate carbamoyltransferase catalytic subunit -
  Q0V31_RS07680 pyrR 1616945..1617451 (-) 507 WP_298186439.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37955.67 Da        Isoelectric Point: 6.8361

>NTDB_id=1154224 Q0V31_RS07655 WP_298186429.1 1611755..1612789(+) (pilT) [MAG: uncultured Pseudomonas sp. isolate AG_MB4]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGPGAVFRTIPSKVLSMEDLGMGEIFRKISDVPRGLVLVTGPTGSGKSTTLAAMLDYINSNKYHHILTVED
PIEFVHESKKCLINQREVHRDTLGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQSVISQTLLKKIGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGSLGMQTLDSCL
KTLVSKGIVSREAAREKAKTPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1154224 Q0V31_RS07655 WP_298186429.1 1611755..1612789(+) (pilT) [MAG: uncultured Pseudomonas sp. isolate AG_MB4]
ATGGATATCACTGAATTGCTGGCCTTCAGCGCCAAGCAAGGTGCATCGGACCTGCACCTCTCCGCTGGTCTGCCCCCGAT
GATTCGGGTCGATGGTGATGTTCGCCGGATCAATTTGCCTGCACTGGACCATAAACAAGTACACGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAAGATTTCGAGGAGTTTCTCGAGACTGACTTCTCCTTTGAAGTGCCGGGTGTGGCGCGT
TTCCGGGTTAACGCCTTCAACCAGAATCGCGGCCCTGGCGCGGTATTCCGCACCATTCCGTCGAAAGTGCTGAGCATGGA
AGACCTCGGTATGGGCGAGATCTTCCGCAAAATATCTGATGTGCCACGCGGCTTGGTGCTGGTGACCGGGCCGACCGGTT
CCGGTAAGTCGACCACCTTGGCGGCGATGCTCGACTACATCAACAGCAATAAGTATCACCACATTCTGACCGTCGAAGAC
CCGATCGAATTCGTTCATGAATCGAAAAAGTGCCTGATCAACCAGCGTGAAGTGCACCGCGACACCCTTGGCTTCTCCGA
AGCCCTGCGCTCGGCGCTGCGTGAAGACCCGGACATCATTCTGGTCGGCGAGATGCGTGACCTGGAAACCATCCGCCTGG
CCCTGACGGCCGCAGAAACGGGGCACCTGGTCTTTGGCACCCTGCACACCACCTCGGCGGCCAAGACCATCGACCGCGTG
GTGGACGTATTCCCTGCCGAAGAAAAGTCCATGGTTCGCTCCATGCTTTCCGAGTCACTGCAGTCGGTTATCTCGCAGAC
CCTGCTGAAGAAAATCGGCGGCGGCCGCGTCGCCGCCCACGAAATCATGATCGGTACCCCGGCCATCCGTAACCTGATCC
GTGAAGACAAGGTGGCGCAGATGTACTCGGCGATTCAGACCGGCGGTTCGCTGGGCATGCAGACCCTGGATTCTTGCCTG
AAAACCCTGGTTTCCAAGGGCATCGTCAGCCGTGAAGCGGCCCGTGAAAAGGCCAAGACCCCGGAAAACTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

93.314

100

0.933
  pilT Pseudomonas aeruginosa PAK

93.314

100

0.933
  pilT Acinetobacter nosocomialis M2

85.174

100

0.852
  pilT Acinetobacter baumannii D1279779

84.884

100

0.849
  pilT Acinetobacter baumannii strain A118

84.884

100

0.849
  pilT Acinetobacter baylyi ADP1

82.849

100

0.828
  pilT Legionella pneumophila strain Lp02

75.444

98.256

0.741
  pilT Legionella pneumophila strain ERS1305867

75.444

98.256

0.741
  pilT Neisseria gonorrhoeae MS11

67.536

100

0.677
  pilT Neisseria meningitidis 8013

67.536

100

0.677
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.546

97.965

0.672
  pilT Vibrio cholerae strain A1552

68.546

97.965

0.672
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

100

0.515
  pilU Vibrio cholerae strain A1552

40.29

100

0.404
  pilU Acinetobacter baylyi ADP1

39.884

100

0.401
  pilU Pseudomonas stutzeri DSM 10701

39.403

97.384

0.384

Multiple sequence alignment