Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   Q0V31_RS07660 Genome accession   NZ_OX044366
Coordinates   1612896..1614041 (+) Length   381 a.a.
NCBI ID   WP_298186431.1    Uniprot ID   -
Organism   MAG: uncultured Pseudomonas sp. isolate AG_MB4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1607896..1619041
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q0V31_RS07635 - 1609241..1609537 (-) 297 WP_298186419.1 DUF167 family protein -
  Q0V31_RS07640 - 1609551..1610141 (-) 591 WP_298186423.1 YggT family protein -
  Q0V31_RS07645 proC 1610151..1610969 (-) 819 WP_298186425.1 pyrroline-5-carboxylate reductase -
  Q0V31_RS07650 - 1611005..1611691 (-) 687 WP_298186427.1 YggS family pyridoxal phosphate-dependent enzyme -
  Q0V31_RS07655 pilT 1611755..1612789 (+) 1035 WP_298186429.1 type IV pilus twitching motility protein PilT Machinery gene
  Q0V31_RS07660 pilU 1612896..1614041 (+) 1146 WP_298186431.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Q0V31_RS07665 - 1614143..1614544 (+) 402 WP_298186432.1 NINE protein -
  Q0V31_RS07670 - 1614653..1615924 (-) 1272 WP_298186434.1 dihydroorotase -
  Q0V31_RS07675 - 1615924..1616934 (-) 1011 WP_298186437.1 aspartate carbamoyltransferase catalytic subunit -
  Q0V31_RS07680 pyrR 1616945..1617451 (-) 507 WP_298186439.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  Q0V31_RS07685 ruvX 1617484..1617927 (-) 444 WP_298186441.1 Holliday junction resolvase RuvX -
  Q0V31_RS07690 - 1617927..1618496 (-) 570 WP_298186444.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 42544.78 Da        Isoelectric Point: 6.6648

>NTDB_id=1154225 Q0V31_RS07660 WP_298186431.1 1612896..1614041(+) (pilU) [MAG: uncultured Pseudomonas sp. isolate AG_MB4]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPITKTPMSPEQTRETVHAVMNEQQRRDFTENHECNFAISARGIGR
FRVSAFYQRNLAGMVLRRIETNIPTLDELKLPEVLKKLALTKRGLVLFVGATGTGKSTSLAAMIGYRNKNSSGHIISIED
PIEYIHQHQSCIVTQREVGIDTESFEVALKNTLRQAPDVILIGEVRTRETMDHAVAFAETGHLCLATLHANNANQALDRI
INFFPADRQNQVWMDLSLNLKAIVAQQLIPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKALMKRSTEQGMQTFDQA
LYNLYSQGEITYEDALLYADSANDLRLMIKLGSETDGDHLSSMAQGLSLEVSDEEPGRRFR

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=1154225 Q0V31_RS07660 WP_298186431.1 1612896..1614041(+) (pilU) [MAG: uncultured Pseudomonas sp. isolate AG_MB4]
ATGGAATTCGAGAAACTGCTGCGTCTGATGGTCGAGAAGGGTGGCTCCGACCTGTTTATTACCGCAGGCGTGCCGCCGTC
GATGAAGGTCAACGGCAAAATTATGCCGATTACCAAGACGCCCATGTCGCCAGAGCAAACCCGTGAAACCGTACACGCGG
TGATGAACGAGCAGCAGCGCCGTGACTTTACTGAGAATCACGAGTGTAACTTCGCCATCAGCGCCCGTGGTATTGGGCGT
TTCCGGGTCAGCGCCTTCTATCAGCGCAACCTGGCCGGCATGGTCTTGCGTCGTATCGAGACCAATATTCCGACCCTGGA
TGAGCTGAAGTTGCCGGAAGTTCTCAAGAAGCTGGCGCTGACCAAGCGCGGGCTGGTGCTCTTTGTGGGTGCCACTGGTA
CCGGTAAGTCGACTTCTCTGGCGGCGATGATCGGCTATCGCAACAAGAACAGTAGCGGTCACATCATCTCCATTGAAGAC
CCGATCGAATACATCCATCAGCATCAGAGCTGCATTGTCACCCAGCGTGAAGTGGGTATCGACACCGAATCGTTTGAGGT
GGCGCTGAAAAACACCCTGCGTCAGGCTCCGGATGTGATCTTGATCGGTGAGGTGCGCACCCGCGAAACCATGGACCATG
CCGTGGCCTTCGCCGAAACCGGGCACCTGTGCCTGGCCACCTTGCACGCCAACAACGCTAACCAGGCGTTGGACCGGATC
ATCAACTTCTTCCCGGCCGATCGGCAGAATCAGGTGTGGATGGACTTGTCGCTGAACCTCAAGGCCATCGTCGCACAGCA
ACTGATACCAACTCCCGATGGCAAAGGCCGGCGTGCGGTGATCGAAGTGTTGATCAACACCCCGCTGGCGGCGGACTTGA
TCCGCAAAGGCGAGGTGCACGAGCTCAAGGCGCTGATGAAGCGCTCCACCGAGCAGGGTATGCAAACCTTCGATCAGGCG
CTGTACAACCTCTACAGCCAGGGCGAGATCACCTACGAAGATGCGCTGCTGTACGCAGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAACCGATGGCGATCACCTGAGCAGCATGGCTCAGGGCCTCTCGTTGGAAGTCAGTGATG
AGGAGCCCGGCCGGCGTTTCCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(110-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

92.388

100

0.924
  pilU Acinetobacter baylyi ADP1

63.866

93.701

0.598
  pilU Vibrio cholerae strain A1552

59.714

91.864

0.549
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.452

88.189

0.383
  pilT Pseudomonas aeruginosa PAK

41.493

87.927

0.365

Multiple sequence alignment