Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LQ108_RS18980 Genome accession   NZ_OW968328
Coordinates   3997226..3998206 (-) Length   326 a.a.
NCBI ID   WP_029883139.1    Uniprot ID   A0AAE2JRL6
Organism   Enterobacter cloacae isolate 1382     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3992226..4003206
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LQ108_RS18950 (AI2999V1_3683) - 3993102..3993599 (+) 498 WP_028027997.1 SprT family zinc-dependent metalloprotease -
  LQ108_RS18955 (AI2999V1_3684) endA 3993694..3994401 (+) 708 WP_148791262.1 deoxyribonuclease I -
  LQ108_RS18960 (AI2999V1_3685) rsmE 3994454..3995185 (+) 732 WP_013098662.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  LQ108_RS18965 (AI2999V1_3686) gshB 3995205..3996152 (+) 948 WP_058680984.1 glutathione synthase -
  LQ108_RS18970 (AI2999V1_3687) - 3996239..3996799 (+) 561 WP_013098664.1 YqgE/AlgH family protein -
  LQ108_RS18975 (AI2999V1_3688) ruvX 3996799..3997215 (+) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  LQ108_RS18980 (AI2999V1_3689) pilT 3997226..3998206 (-) 981 WP_029883139.1 type IV pilus twitching motility protein PilT Machinery gene
  LQ108_RS18985 (AI2999V1_3690) - 3998224..3998925 (+) 702 WP_023620785.1 YggS family pyridoxal phosphate-dependent enzyme -
  LQ108_RS18990 (AI2999V1_3691) - 3998947..3999513 (+) 567 WP_013098667.1 YggT family protein -
  LQ108_RS18995 (AI2999V1_3692) yggU 3999510..3999797 (+) 288 WP_020687057.1 DUF167 family protein YggU -
  LQ108_RS19000 (AI2999V1_3693) - 3999810..4000403 (+) 594 WP_058680986.1 XTP/dITP diphosphatase -
  LQ108_RS19005 (AI2999V1_3694) hemW 4000396..4001544 (+) 1149 WP_020687055.1 radical SAM family heme chaperone HemW -
  LQ108_RS19010 (AI2999V1_3695) - 4001601..4001939 (+) 339 WP_040021717.1 endonuclease domain-containing protein -
  LQ108_RS19015 (AI2999V1_3696) - 4002022..4002738 (-) 717 WP_023621456.1 DUF2884 domain-containing protein -
  LQ108_RS19020 (AI2999V1_3697) - 4002795..4003121 (-) 327 WP_003862421.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35652.66 Da        Isoelectric Point: 6.3038

>NTDB_id=1153459 LQ108_RS18980 WP_029883139.1 3997226..3998206(-) (pilT) [Enterobacter cloacae isolate 1382]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRVGRLEPAPFPSPDVDALLKTWLNDEQQGAWWASGQVDFAVTLTGNQRLRA
SAFKQMKGNSITLRLLPRACPQLSALGVPRAIPELLSHDSGLILVTGATGSGKSTTLAAMVDFLNHQTDGHILTLEDPVE
FVYQSERCLIQQREIGLHSPSFAEALRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQRLLPDLQGGRVALYELLVNTPAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=1153459 LQ108_RS18980 WP_029883139.1 3997226..3998206(-) (pilT) [Enterobacter cloacae isolate 1382]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAATGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGAGTAGGCCGCCTTGAACCGGCTCCCTTTCCCTCTCCCGATGTGGATGCGTTACTGAAAACGTGGCTCAACG
ATGAGCAGCAGGGGGCATGGTGGGCAAGCGGGCAGGTGGATTTTGCCGTGACCCTTACGGGAAACCAACGCCTGCGCGCA
AGCGCCTTTAAACAGATGAAGGGCAACTCCATCACGCTACGCCTGTTGCCCCGGGCCTGTCCGCAGCTCTCCGCCCTGGG
GGTACCCCGGGCTATCCCGGAACTCTTATCCCACGACAGTGGGCTGATACTGGTGACGGGGGCAACCGGCAGCGGCAAAT
CTACCACCCTTGCGGCAATGGTGGATTTTCTTAATCACCAGACGGATGGGCATATTCTGACGCTGGAAGATCCGGTGGAG
TTTGTCTATCAGAGTGAACGTTGCCTGATTCAGCAGCGAGAGATTGGCCTGCACAGTCCCTCATTTGCCGAGGCGCTGCG
CGCTGCGTTGCGTGAAGATCCGGATGTCATCCTGCTGGGAGAACTGCGCGACAGCGAAACGATCCGCCTGGCGCTGACGG
CCGCTGAAACCGGGCATCTGGTGCTGGCGACGCTGCACACGCGCGGTGCCTCTCAGGCGATTGAACGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGACCCGGTGCGTAATCAGCTGGCCGGCAGCCTGCGGGCGGTGCTGGCGCAAAGACTGCTTCC
CGACCTGCAGGGTGGGCGCGTCGCTCTGTATGAACTGCTGGTGAATACGCCAGCGGCAGCGAACCTTATCCGCGAAGGTA
AAACGTGGCAGTTGCCCGGGATTATTCAAACCGGTCAGCAGGCGGGGATGCAGAACTTTGACCAGAGCCTGGCAGAGCGA
CGGGCGCAGGGGCGATTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(63-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.153

100

0.503
  pilT Vibrio cholerae strain A1552

50.153

100

0.503
  pilT Pseudomonas stutzeri DSM 10701

48.624

100

0.488
  pilT Legionella pneumophila strain ERS1305867

49.068

98.773

0.485
  pilT Legionella pneumophila strain Lp02

49.068

98.773

0.485
  pilT Acinetobacter baumannii strain A118

48.012

100

0.482
  pilT Acinetobacter baumannii D1279779

48.012

100

0.482
  pilT Acinetobacter baylyi ADP1

47.706

100

0.479
  pilT Pseudomonas aeruginosa PAK

47.706

100

0.479
  pilT Acinetobacter nosocomialis M2

48.438

98.16

0.475
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.168

98.773

0.426
  pilU Pseudomonas stutzeri DSM 10701

38.788

100

0.393
  pilU Vibrio cholerae strain A1552

39.441

98.773

0.39
  pilB Haemophilus influenzae 86-028NP

33.708

100

0.368
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362

Multiple sequence alignment