Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MKZ32_RS01085 Genome accession   NZ_OU912926
Coordinates   241429..242472 (+) Length   347 a.a.
NCBI ID   WP_239795574.1    Uniprot ID   -
Organism   Candidatus Nitrotoga arctica strain 6680 isolate 6680     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 236429..247472
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKZ32_RS01055 (NTG6680_0214) - 237228..237509 (+) 282 WP_239795568.1 hypothetical protein -
  MKZ32_RS01060 (NTG6680_0215) - 238138..238590 (+) 453 WP_239795569.1 hypothetical protein -
  MKZ32_RS01065 (NTG6680_0216) - 238893..239159 (-) 267 WP_239795570.1 DUF167 domain-containing protein -
  MKZ32_RS01070 (NTG6680_0217) - 239198..239761 (-) 564 WP_239795571.1 YggT family protein -
  MKZ32_RS01075 (NTG6680_0218) proC 239755..240570 (-) 816 WP_239795572.1 pyrroline-5-carboxylate reductase -
  MKZ32_RS01080 (NTG6680_0219) - 240567..241265 (-) 699 WP_239795573.1 YggS family pyridoxal phosphate-dependent enzyme -
  MKZ32_RS01085 (NTG6680_0220) pilT 241429..242472 (+) 1044 WP_239795574.1 type IV pilus twitching motility protein PilT Machinery gene
  MKZ32_RS01090 (NTG6680_0221) pilU 242498..243634 (+) 1137 WP_239795575.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MKZ32_RS01095 (NTG6680_0223) phoU 243821..244528 (-) 708 WP_239795576.1 phosphate signaling complex protein PhoU -
  MKZ32_RS01100 (NTG6680_0224) rpiA 244596..245255 (-) 660 WP_239795577.1 ribose-5-phosphate isomerase RpiA -
  MKZ32_RS01105 (NTG6680_0225) - 245502..246059 (+) 558 WP_239795578.1 class I SAM-dependent methyltransferase -
  MKZ32_RS15585 - 246162..246389 (-) 228 WP_420887750.1 helix-turn-helix domain-containing protein -
  MKZ32_RS15590 - 246441..246809 (-) 369 Protein_223 response regulator -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38168.18 Da        Isoelectric Point: 7.3504

>NTDB_id=1151573 MKZ32_RS01085 WP_239795574.1 241429..242472(+) (pilT) [Candidatus Nitrotoga arctica strain 6680 isolate 6680]
MDITELLAFGVKNKASDLHLSAGLPPMIRVHGDIRKINLPAMLHTEVHAMVYDIMNDGQRKIYEENKEIDFSFEIPGLAR
FRVNAFVHQRGAGAVMRTIPSKILTLADLKAPKILESIADYPRGMVLVTGPTGSGKSTTLAAMVNHRNEHEMGHILTVED
PIEFVHESKKCLVNQREVGAHTLSFQNALRSALREDPDIILVGEMRDLETIRLAMSAAETGHLVFGTLHTSSAAKTIDRI
IDVFPADEKEMVRAMLSESLRAVISQTLLKTKDGTGRVAAHEIMICTPAIRNLIREAKVPQMYSSIQTGGALGMQTLDQC
LQDLVKRNVVSSAEARTKAANKDTFVG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1151573 MKZ32_RS01085 WP_239795574.1 241429..242472(+) (pilT) [Candidatus Nitrotoga arctica strain 6680 isolate 6680]
ATGGACATTACCGAACTGCTTGCTTTTGGTGTCAAAAACAAGGCTTCCGATTTGCACCTATCTGCTGGGTTGCCGCCGAT
GATTCGTGTGCATGGTGACATACGCAAAATCAACCTGCCAGCGATGCTACATACGGAAGTGCACGCCATGGTATATGACA
TCATGAACGATGGGCAGCGCAAAATTTATGAAGAAAATAAAGAAATCGATTTCTCTTTTGAGATCCCGGGACTCGCACGT
TTTCGCGTAAATGCCTTTGTCCATCAGCGCGGTGCAGGTGCGGTGATGCGTACCATTCCATCTAAAATATTGACCTTGGC
AGACCTCAAGGCCCCCAAAATTTTGGAGTCTATTGCCGATTATCCGCGTGGCATGGTGTTAGTGACTGGCCCGACTGGAT
CGGGTAAGTCTACCACGTTGGCCGCGATGGTTAATCATCGCAATGAACATGAAATGGGGCACATCCTGACCGTGGAGGAT
CCGATTGAATTTGTCCACGAAAGTAAAAAGTGTTTGGTCAACCAGCGCGAAGTCGGCGCGCATACTTTGTCATTTCAAAA
CGCTTTACGTAGCGCATTGCGCGAAGACCCGGACATTATCCTGGTAGGTGAAATGCGCGACTTAGAAACGATACGCCTGG
CCATGTCTGCGGCTGAAACCGGTCATTTGGTATTTGGAACACTCCATACCAGTTCTGCGGCCAAGACCATCGACCGCATC
ATTGACGTTTTCCCCGCGGACGAAAAGGAAATGGTGCGCGCCATGCTGTCAGAATCACTGCGAGCAGTCATTTCCCAAAC
CTTGCTTAAAACCAAAGACGGTACCGGACGTGTGGCAGCCCATGAAATTATGATTTGCACTCCAGCTATCCGTAATCTGA
TTCGTGAAGCCAAGGTGCCACAGATGTATTCCTCTATCCAGACCGGGGGTGCTCTTGGAATGCAGACGCTAGACCAATGC
CTGCAAGATCTGGTCAAGCGCAACGTTGTATCATCTGCAGAAGCGCGCACCAAGGCAGCTAACAAAGATACGTTTGTCGG
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

73.988

99.712

0.738
  pilT Acinetobacter baumannii D1279779

73.988

99.712

0.738
  pilT Acinetobacter baumannii strain A118

73.988

99.712

0.738
  pilT Pseudomonas stutzeri DSM 10701

73.913

99.424

0.735
  pilT Acinetobacter baylyi ADP1

73.333

99.424

0.729
  pilT Pseudomonas aeruginosa PAK

72.754

99.424

0.723
  pilT Legionella pneumophila strain Lp02

71.304

99.424

0.709
  pilT Legionella pneumophila strain ERS1305867

71.304

99.424

0.709
  pilT Neisseria meningitidis 8013

69.565

99.424

0.692
  pilT Neisseria gonorrhoeae MS11

69.275

99.424

0.689
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.751

97.406

0.66
  pilT Vibrio cholerae strain A1552

67.751

97.406

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

99.424

0.496
  pilU Pseudomonas stutzeri DSM 10701

43.284

96.542

0.418
  pilU Acinetobacter baylyi ADP1

42.56

96.83

0.412
  pilU Vibrio cholerae strain A1552

42.09

96.542

0.406

Multiple sequence alignment