Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MKZ32_RS01090 Genome accession   NZ_OU912926
Coordinates   242498..243634 (+) Length   378 a.a.
NCBI ID   WP_239795575.1    Uniprot ID   -
Organism   Candidatus Nitrotoga arctica strain 6680 isolate 6680     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 237498..248634
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKZ32_RS01060 (NTG6680_0215) - 238138..238590 (+) 453 WP_239795569.1 hypothetical protein -
  MKZ32_RS01065 (NTG6680_0216) - 238893..239159 (-) 267 WP_239795570.1 DUF167 domain-containing protein -
  MKZ32_RS01070 (NTG6680_0217) - 239198..239761 (-) 564 WP_239795571.1 YggT family protein -
  MKZ32_RS01075 (NTG6680_0218) proC 239755..240570 (-) 816 WP_239795572.1 pyrroline-5-carboxylate reductase -
  MKZ32_RS01080 (NTG6680_0219) - 240567..241265 (-) 699 WP_239795573.1 YggS family pyridoxal phosphate-dependent enzyme -
  MKZ32_RS01085 (NTG6680_0220) pilT 241429..242472 (+) 1044 WP_239795574.1 type IV pilus twitching motility protein PilT Machinery gene
  MKZ32_RS01090 (NTG6680_0221) pilU 242498..243634 (+) 1137 WP_239795575.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MKZ32_RS01095 (NTG6680_0223) phoU 243821..244528 (-) 708 WP_239795576.1 phosphate signaling complex protein PhoU -
  MKZ32_RS01100 (NTG6680_0224) rpiA 244596..245255 (-) 660 WP_239795577.1 ribose-5-phosphate isomerase RpiA -
  MKZ32_RS01105 (NTG6680_0225) - 245502..246059 (+) 558 WP_239795578.1 class I SAM-dependent methyltransferase -
  MKZ32_RS15585 - 246162..246389 (-) 228 WP_420887750.1 helix-turn-helix domain-containing protein -
  MKZ32_RS15590 - 246441..246809 (-) 369 Protein_223 response regulator -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42213.73 Da        Isoelectric Point: 5.9638

>NTDB_id=1151574 MKZ32_RS01090 WP_239795575.1 242498..243634(+) (pilU) [Candidatus Nitrotoga arctica strain 6680 isolate 6680]
MERDQATELMHNLLRGMITQKASDLFITTGFPPAFKVDGKMTPVSQQVLTPQHTMELANSIMNDRQAAEFEASHECNFAI
SPAGIGRFRVNVFMQQQRIGMVLRTITTKIPDLTQMGMPEVLKDVAMTKRGLVVLVGGTGSGKSTTLAAMLGYRNQNTYG
HIITIEDPVEFVHEHINCLITHREVGVDTDSWQVALKNTLRQAPDVIFIGEIRDRETMEYAVTFAETGHLCMATLHANSA
NQALDRIINFFPEERREQLLMDLSLNIKALISQRLIPRVDGIGRSVAMEIMLNSPLISDLIFKGEVLAIKGVMSKSRELG
MQTFDQALFDLHEANLISYAEALKNADSVNDLRLRIKLESQSSKDIDIMAGLDHLQMT

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=1151574 MKZ32_RS01090 WP_239795575.1 242498..243634(+) (pilU) [Candidatus Nitrotoga arctica strain 6680 isolate 6680]
ATGGAAAGAGATCAGGCTACCGAGCTAATGCATAATCTGTTGCGAGGGATGATTACCCAGAAGGCATCTGACCTGTTTAT
TACAACCGGATTCCCACCCGCATTCAAGGTGGACGGCAAGATGACCCCCGTTTCACAACAGGTGCTGACACCGCAACACA
CTATGGAATTAGCGAATAGCATCATGAACGACCGCCAGGCCGCTGAATTCGAAGCGTCACACGAATGCAACTTTGCGATC
AGTCCGGCTGGCATTGGTCGCTTCCGCGTTAATGTGTTTATGCAACAGCAGCGTATAGGCATGGTGTTACGCACCATCAC
TACGAAGATTCCCGATCTTACTCAAATGGGGATGCCAGAAGTACTAAAGGATGTGGCAATGACCAAACGTGGACTGGTCG
TTCTGGTCGGTGGTACTGGCAGTGGCAAATCCACAACATTGGCAGCCATGCTTGGCTACCGTAACCAGAATACGTATGGT
CACATCATTACCATCGAAGATCCGGTTGAGTTTGTGCATGAGCACATCAACTGTCTCATCACTCACCGCGAAGTTGGAGT
GGACACGGATTCTTGGCAAGTCGCATTGAAAAACACGTTGCGCCAAGCGCCGGATGTCATTTTCATCGGTGAGATACGCG
ACCGTGAAACCATGGAATATGCTGTGACATTTGCTGAGACCGGCCATCTGTGTATGGCCACATTACACGCCAACAGTGCG
AATCAGGCACTGGATCGCATTATCAATTTCTTCCCGGAAGAGCGTCGCGAGCAATTGTTAATGGATTTATCTCTCAATAT
CAAGGCACTTATTTCACAGCGCCTAATCCCGCGTGTGGATGGGATAGGTCGTTCAGTGGCCATGGAAATCATGCTCAACT
CACCGCTCATCTCTGATTTGATTTTTAAGGGCGAGGTGCTTGCAATCAAGGGAGTGATGTCTAAATCGCGTGAGCTTGGG
ATGCAGACCTTCGATCAAGCGCTGTTCGATCTGCATGAAGCCAACCTGATCAGTTATGCAGAAGCGTTAAAGAATGCTGA
CTCGGTCAATGATCTGCGCCTGCGTATTAAGCTGGAAAGTCAAAGTTCCAAGGATATCGACATAATGGCGGGGCTGGATC
ACTTGCAGATGACTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(87-256)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.143

92.593

0.566
  pilU Acinetobacter baylyi ADP1

57.103

94.974

0.542
  pilU Vibrio cholerae strain A1552

55.747

92.063

0.513
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.776

88.624

0.397
  pilT Pseudomonas aeruginosa PAK

44.343

86.508

0.384
  pilT Pseudomonas stutzeri DSM 10701

43.731

86.508

0.378
  pilT Acinetobacter baylyi ADP1

41.964

88.889

0.373
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.949

83.069

0.365
  pilT Vibrio cholerae strain A1552

43.949

83.069

0.365
  pilT Acinetobacter baumannii D1279779

42.025

86.243

0.362
  pilT Acinetobacter baumannii strain A118

42.025

86.243

0.362
  pilT Legionella pneumophila strain Lp02

42.025

86.243

0.362
  pilT Acinetobacter nosocomialis M2

42.025

86.243

0.362
  pilT Legionella pneumophila strain ERS1305867

42.025

86.243

0.362

Multiple sequence alignment