Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KOD61_RS03880 Genome accession   NZ_OU015430
Coordinates   835980..837017 (+) Length   345 a.a.
NCBI ID   WP_215219744.1    Uniprot ID   -
Organism   Novilysobacter luteus strain CECT 30171     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 830980..842017
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KOD61_RS03850 (LYB30171_00777) - 831427..832224 (+) 798 WP_215219738.1 cobalamin-binding protein -
  KOD61_RS03855 (LYB30171_00778) - 832305..832685 (+) 381 WP_215219739.1 PA2779 family protein -
  KOD61_RS03860 (LYB30171_00779) - 832730..833692 (+) 963 WP_215219740.1 PA2778 family cysteine peptidase -
  KOD61_RS03865 (LYB30171_00780) - 833696..834172 (-) 477 WP_215219741.1 DUF4426 domain-containing protein -
  KOD61_RS03870 (LYB30171_00781) proC 834182..835024 (-) 843 WP_215219742.1 pyrroline-5-carboxylate reductase -
  KOD61_RS03875 (LYB30171_00782) - 835088..835771 (-) 684 WP_215219743.1 YggS family pyridoxal phosphate-dependent enzyme -
  KOD61_RS03880 (LYB30171_00783) pilT 835980..837017 (+) 1038 WP_215219744.1 type IV pilus twitching motility protein PilT Machinery gene
  KOD61_RS03885 (LYB30171_00784) pilU 837075..838256 (+) 1182 WP_215219745.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KOD61_RS03890 (LYB30171_00785) - 838264..839202 (-) 939 WP_215219746.1 DUF72 domain-containing protein -
  KOD61_RS03895 (LYB30171_00786) - 839245..841005 (-) 1761 WP_215219747.1 dipeptidyl-peptidase 3 family protein -
  KOD61_RS03900 (LYB30171_00787) - 841065..841457 (-) 393 WP_215219748.1 response regulator transcription factor -
  KOD61_RS03905 (LYB30171_00788) - 841471..841911 (-) 441 WP_215219749.1 response regulator -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38432.18 Da        Isoelectric Point: 6.7297

>NTDB_id=1150990 KOD61_RS03880 WP_215219744.1 835980..837017(+) (pilT) [Novilysobacter luteus strain CECT 30171]
MDIAELLAFSVKNKASDLHLSAGMPPMIRVDGDVRRINIPALDHKAVHALVYDIMSDKQRRDFEEFLEVDFSFEIPGLAR
FRVNAFNQNRGAGAVFRTIPSDILSLEDLGTPRIFKELIDQPQGLILVTGPTGSGKSTTLAAMIDHINKNEYAHILSVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDYILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTVDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQQSGMMTLDQHL
QDLVKRGLILKPQAREYAKDKRLFD

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1150990 KOD61_RS03880 WP_215219744.1 835980..837017(+) (pilT) [Novilysobacter luteus strain CECT 30171]
ATGGATATCGCCGAACTGTTGGCGTTTTCGGTCAAGAACAAGGCATCCGACCTGCACCTGTCGGCGGGCATGCCGCCGAT
GATCCGGGTCGACGGCGACGTCCGCCGCATCAACATCCCGGCGCTGGACCACAAGGCGGTGCACGCGCTGGTCTACGACA
TCATGTCGGACAAGCAGCGGCGCGACTTCGAGGAGTTCCTCGAGGTCGACTTCTCCTTCGAGATCCCGGGCCTGGCCCGC
TTCCGCGTCAACGCGTTCAACCAGAACCGCGGCGCCGGCGCGGTGTTCCGCACGATTCCGTCGGACATCCTGTCGCTGGA
GGACCTCGGCACGCCGCGGATCTTCAAGGAACTGATCGACCAGCCGCAGGGCCTGATCCTGGTGACCGGTCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCGACCACATCAACAAGAACGAGTACGCGCACATCCTCTCGGTCGAGGAT
CCGATCGAGTTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTCCACCGCGACACCCACGGCTTCAACGA
GGCGCTGCGTTCGGCGCTGCGCGAGGACCCCGACTACATCCTGGTCGGCGAGTTGCGCGACCTGGAGACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCTCGTCGGCGGCCAAGACCGTCGACCGCATC
ATCGACGTGTTCCCCGCCGGCGAGAAGCCGATGGTGCGCTCGATGCTGTCGGAGTCGCTGCGCGCGGTGATCTCGCAGGC
GCTGCTGAAGAAGGTCGGCGGTGGCCGCACCGCCGCCTGGGAAATCATGGTCGGCACCCCGGCCATCCGCAACCTGATCC
GCGAGGACAAGGTCGCGCAGATGTATTCGGCGATCCAGACCGGCCAGCAGTCCGGAATGATGACCCTGGACCAGCACTTG
CAGGACCTCGTCAAGCGCGGCCTGATCCTCAAGCCGCAGGCCCGTGAGTACGCCAAGGACAAGCGCCTGTTCGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

79.36

99.71

0.791
  pilT Acinetobacter baumannii strain A118

79.36

99.71

0.791
  pilT Acinetobacter nosocomialis M2

79.36

99.71

0.791
  pilT Acinetobacter baylyi ADP1

77.035

99.71

0.768
  pilT Pseudomonas stutzeri DSM 10701

77.035

99.71

0.768
  pilT Pseudomonas aeruginosa PAK

77.876

98.261

0.765
  pilT Legionella pneumophila strain Lp02

75

99.71

0.748
  pilT Legionella pneumophila strain ERS1305867

75

99.71

0.748
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.213

95.362

0.67
  pilT Vibrio cholerae strain A1552

70.213

95.362

0.67
  pilT Neisseria meningitidis 8013

66.276

98.841

0.655
  pilT Neisseria gonorrhoeae MS11

65.982

98.841

0.652
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.696

100

0.487
  pilU Acinetobacter baylyi ADP1

40.882

98.551

0.403
  pilU Pseudomonas stutzeri DSM 10701

41.818

95.652

0.4
  pilU Vibrio cholerae strain A1552

40.615

94.203

0.383

Multiple sequence alignment