Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   HUW69_RS11770 Genome accession   NZ_LT992490
Coordinates   2634282..2635319 (-) Length   345 a.a.
NCBI ID   WP_071179069.1    Uniprot ID   -
Organism   Vibrio cholerae strain 4295STDY6534200     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2629282..2640319
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUW69_RS11745 (SAMEA4374365_02392) - 2629766..2630329 (+) 564 WP_001054771.1 YqgE/AlgH family protein -
  HUW69_RS11750 (SAMEA4374365_02393) ruvX 2630410..2630832 (+) 423 WP_000091869.1 Holliday junction resolvase RuvX -
  HUW69_RS11755 (SAMEA4374365_02394) tyrS 2630857..2632137 (-) 1281 WP_174566577.1 tyrosine--tRNA ligase -
  HUW69_RS11760 (SAMEA4374365_02395) - 2632246..2633043 (-) 798 WP_174566578.1 helix-turn-helix transcriptional regulator -
  HUW69_RS11765 (SAMEA4374365_02396) pilU 2633161..2634267 (-) 1107 WP_032478770.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HUW69_RS11770 (SAMEA4374365_02397) pilT 2634282..2635319 (-) 1038 WP_071179069.1 type IV pilus twitching motility protein PilT Machinery gene
  HUW69_RS11775 (SAMEA4374365_02398) - 2635345..2636055 (+) 711 WP_029627832.1 YggS family pyridoxal phosphate-dependent enzyme -
  HUW69_RS11780 (SAMEA4374365_02399) proC 2636108..2636926 (+) 819 WP_071179067.1 pyrroline-5-carboxylate reductase -
  HUW69_RS11785 (SAMEA4374365_02400) - 2636980..2637537 (+) 558 WP_001087261.1 YggT family protein -
  HUW69_RS11790 (SAMEA4374365_02401) yggU 2637537..2637827 (+) 291 WP_000741914.1 DUF167 family protein YggU -
  HUW69_RS11795 (SAMEA4374365_02402) - 2637873..2638304 (+) 432 WP_001233673.1 DUF4426 domain-containing protein -
  HUW69_RS11800 (SAMEA4374365_02403) - 2638374..2638976 (+) 603 WP_000725010.1 XTP/dITP diphosphatase -
  HUW69_RS11805 (SAMEA4374365_02404) hemW 2638970..2640145 (+) 1176 WP_071179065.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38208.81 Da        Isoelectric Point: 6.6672

>NTDB_id=1150287 HUW69_RS11770 WP_071179069.1 2634282..2635319(-) (pilT) [Vibrio cholerae strain 4295STDY6534200]
MDIAELLEFSVKHNASDLHLSAGVPPMVRIDGEVRKLGVPAFTHSDVHRLIFEIMNDGQRSEYEEKLEVDFSFELPSVGR
FRVNAFHQARGCSAVFRTIPTVIPTLEQLDAPEIFSKIANYEKGLVLVTGPTGSGKSTTLAAMVNYVNAHHNKHILTIED
PIEFVHSNNKCLINQREVHRDTHSFKNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRAVIAQKLLKRVGGGRVACHEIMLATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLMARGVVDAQEVQSKIELDLKAF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1150287 HUW69_RS11770 WP_071179069.1 2634282..2635319(-) (pilT) [Vibrio cholerae strain 4295STDY6534200]
ATGGATATCGCTGAGTTACTGGAATTTAGTGTAAAGCATAACGCCTCAGATCTACATCTTTCCGCAGGTGTTCCGCCTAT
GGTACGGATTGATGGCGAAGTTAGGAAGCTTGGCGTGCCTGCTTTTACCCATTCTGATGTACACCGTTTGATTTTTGAGA
TTATGAACGATGGCCAACGCAGTGAATATGAAGAGAAATTAGAAGTCGATTTTTCTTTTGAACTGCCTAGTGTTGGCCGT
TTCCGGGTTAACGCGTTTCATCAAGCGCGCGGATGCTCGGCGGTGTTTCGTACCATTCCCACTGTTATCCCGACGTTAGA
GCAATTGGATGCTCCTGAGATCTTCAGCAAAATTGCCAATTATGAAAAAGGGTTAGTTTTAGTGACTGGGCCAACGGGTT
CGGGTAAGTCGACGACCTTGGCGGCGATGGTGAACTATGTCAATGCCCATCACAATAAGCATATTTTGACTATTGAAGAT
CCGATTGAATTTGTGCACAGCAACAATAAGTGTTTGATCAACCAGCGCGAAGTGCACCGTGATACCCACAGTTTTAAAAA
TGCATTGCGTTCGGCATTGCGTGAAGACCCAGATGTGATCCTCGTCGGTGAGCTGCGTGACCAAGAAACGATTAGCTTGG
CGCTTACCGCGGCAGAAACCGGTCACTTGGTGTTTGGCACTCTGCACACCAGCTCGGCGGCGAAAACCATTGACCGGATT
ATCGATGTTTTTCCGGGCAGCGACAAAGACATGGTGCGTTCCATGCTGTCGGAATCGCTGCGTGCGGTTATCGCGCAAAA
GCTCCTAAAACGCGTCGGGGGTGGTCGTGTTGCGTGTCATGAAATCATGCTGGCAACGCCAGCGATCCGGAACTTGATCC
GTGAAGATAAAGTGGCGCAGATGTATTCGATCATCCAAACCGGTGCCGCACACGGCATGCAAACTATGGAGCAAAACGCT
AAACAGCTGATGGCGCGTGGTGTGGTGGATGCGCAAGAAGTGCAGAGCAAAATCGAGTTGGACTTAAAAGCATTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

99.13

100

0.991
  pilT Vibrio cholerae strain A1552

99.13

100

0.991
  pilT Acinetobacter baumannii D1279779

71.429

97.391

0.696
  pilT Acinetobacter baumannii strain A118

71.429

97.391

0.696
  pilT Acinetobacter nosocomialis M2

71.131

97.391

0.693
  pilT Acinetobacter baylyi ADP1

72.121

95.652

0.69
  pilT Pseudomonas stutzeri DSM 10701

68.843

97.681

0.672
  pilT Pseudomonas aeruginosa PAK

66.765

98.551

0.658
  pilT Legionella pneumophila strain Lp02

67.173

95.362

0.641
  pilT Legionella pneumophila strain ERS1305867

67.173

95.362

0.641
  pilT Neisseria meningitidis 8013

64.242

95.652

0.614
  pilT Neisseria gonorrhoeae MS11

63.939

95.652

0.612
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.988

94.783

0.493
  pilU Vibrio cholerae strain A1552

42.216

96.812

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.828

97.971

0.4
  pilU Acinetobacter baylyi ADP1

40.625

92.754

0.377

Multiple sequence alignment