Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   HUW69_RS11765 Genome accession   NZ_LT992490
Coordinates   2633161..2634267 (-) Length   368 a.a.
NCBI ID   WP_032478770.1    Uniprot ID   -
Organism   Vibrio cholerae strain 4295STDY6534200     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2628161..2639267
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUW69_RS11740 (SAMEA4374365_02391) gshB 2628774..2629730 (+) 957 WP_000593313.1 glutathione synthase -
  HUW69_RS11745 (SAMEA4374365_02392) - 2629766..2630329 (+) 564 WP_001054771.1 YqgE/AlgH family protein -
  HUW69_RS11750 (SAMEA4374365_02393) ruvX 2630410..2630832 (+) 423 WP_000091869.1 Holliday junction resolvase RuvX -
  HUW69_RS11755 (SAMEA4374365_02394) tyrS 2630857..2632137 (-) 1281 WP_174566577.1 tyrosine--tRNA ligase -
  HUW69_RS11760 (SAMEA4374365_02395) - 2632246..2633043 (-) 798 WP_174566578.1 helix-turn-helix transcriptional regulator -
  HUW69_RS11765 (SAMEA4374365_02396) pilU 2633161..2634267 (-) 1107 WP_032478770.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  HUW69_RS11770 (SAMEA4374365_02397) pilT 2634282..2635319 (-) 1038 WP_071179069.1 type IV pilus twitching motility protein PilT Machinery gene
  HUW69_RS11775 (SAMEA4374365_02398) - 2635345..2636055 (+) 711 WP_029627832.1 YggS family pyridoxal phosphate-dependent enzyme -
  HUW69_RS11780 (SAMEA4374365_02399) proC 2636108..2636926 (+) 819 WP_071179067.1 pyrroline-5-carboxylate reductase -
  HUW69_RS11785 (SAMEA4374365_02400) - 2636980..2637537 (+) 558 WP_001087261.1 YggT family protein -
  HUW69_RS11790 (SAMEA4374365_02401) yggU 2637537..2637827 (+) 291 WP_000741914.1 DUF167 family protein YggU -
  HUW69_RS11795 (SAMEA4374365_02402) - 2637873..2638304 (+) 432 WP_001233673.1 DUF4426 domain-containing protein -
  HUW69_RS11800 (SAMEA4374365_02403) - 2638374..2638976 (+) 603 WP_000725010.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41337.30 Da        Isoelectric Point: 6.5076

>NTDB_id=1150286 HUW69_RS11765 WP_032478770.1 2633161..2634267(-) (pilU) [Vibrio cholerae strain 4295STDY6534200]
MELNQYLDGMLTHKASDLYITVGAPILYRVDGELRAQGEALSVADVTALLHAMMDDARQAEFKQTREANFAVVRDSGRFR
VSAFFQRELPGAVIRRIETRIPTFEELKLPEVLQNLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNQHRTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQREQFLLDLSLNLKGVIAQQLLRDKNGKGRHGVFEILLNSPRISDLIRRGELHELKATMARSQEVGMQTFDQALY
QLVVDDKISEQDALHSADSANDLRLMLKTKRGDDYGSGSLQNVKIDME

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=1150286 HUW69_RS11765 WP_032478770.1 2633161..2634267(-) (pilU) [Vibrio cholerae strain 4295STDY6534200]
ATGGAGTTGAATCAATATCTGGATGGCATGCTGACTCATAAAGCATCGGATCTGTACATTACGGTGGGGGCACCGATTTT
GTATCGAGTCGATGGTGAATTGCGTGCGCAAGGTGAAGCGCTCAGCGTGGCGGATGTGACGGCCTTATTGCATGCGATGA
TGGATGACGCAAGGCAAGCAGAATTTAAGCAGACGCGCGAAGCGAACTTTGCTGTGGTGCGTGACAGTGGCCGTTTTCGG
GTCAGTGCATTTTTCCAACGTGAATTGCCGGGGGCGGTGATCCGCCGGATTGAAACGCGCATTCCCACCTTTGAAGAACT
GAAATTGCCTGAAGTGCTGCAAAATTTGGCGATTGCCAAACGCGGTTTAGTGTTGGTGGTCGGCGCAACCGGTTCGGGTA
AATCGACCACTATGGCAGCCATGACTGGCTATCGTAATCAGCATCGCACCGGGCATATTTTGACGGTCGAAGATCCAATT
GAGTTTGTGCATGAACACAAGCGCTGTATCGTGACGCAGCGAGAAGTGGGGCTCGATACCGAAAGTTATGAAGTGGCGCT
GAAAAACTCGCTGCGCCAAGCCCCCGATATGATTTTGATTGGCGAAATCCGTAGCCGAGAAACCATGGAATACGCGATGA
CTTTCGCGGAAACCGGGCATTTGTGTATGGCCACTTTGCACGCCAATAACGCCAACCAAGCATTAGAGCGGATTTTGCAT
CTGGTGCCCAAAGAGCAGCGTGAGCAGTTTTTGCTCGATTTGTCACTCAACCTCAAAGGCGTGATTGCTCAGCAATTATT
GCGCGATAAAAATGGCAAAGGTCGACACGGGGTGTTTGAAATTCTGCTCAATAGCCCGCGCATTTCCGATTTGATCCGCC
GTGGCGAGCTACACGAGCTGAAAGCGACCATGGCTCGTTCGCAGGAAGTGGGGATGCAAACCTTCGATCAAGCCTTATAC
CAGCTTGTGGTGGATGACAAAATCAGTGAGCAAGATGCCCTGCACAGTGCCGATTCAGCCAACGATCTGCGTTTGATGCT
CAAAACCAAGCGTGGTGATGACTACGGCAGTGGCTCTTTGCAGAACGTGAAGATTGATATGGAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

99.457

100

0.995
  pilU Pseudomonas stutzeri DSM 10701

58.286

95.109

0.554
  pilU Acinetobacter baylyi ADP1

54.701

95.38

0.522
  pilT Legionella pneumophila strain Lp02

43.068

92.12

0.397
  pilT Legionella pneumophila strain ERS1305867

43.068

92.12

0.397
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.129

84.239

0.389
  pilT Acinetobacter baumannii D1279779

41.888

92.12

0.386
  pilT Acinetobacter baumannii strain A118

41.888

92.12

0.386
  pilT Acinetobacter nosocomialis M2

41.888

92.12

0.386
  pilT Vibrio cholerae strain A1552

42.515

90.761

0.386
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.515

90.761

0.386
  pilT Pseudomonas aeruginosa PAK

41.593

92.12

0.383
  pilT Pseudomonas stutzeri DSM 10701

41.298

92.12

0.38
  pilT Acinetobacter baylyi ADP1

42.769

88.315

0.378
  pilT Neisseria meningitidis 8013

38.841

93.75

0.364
  pilT Neisseria gonorrhoeae MS11

38.617

94.293

0.364

Multiple sequence alignment