Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   VTAP4600_RS00210 Genome accession   NZ_LT960611
Coordinates   33459..34496 (+) Length   345 a.a.
NCBI ID   WP_102520949.1    Uniprot ID   A0A2N8Z7X0
Organism   Vibrio tapetis subsp. tapetis isolate Vibrio tapetis CECT4600     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 28459..39496
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VTAP4600_RS00175 (VTAP4600_A0029) hemW 28718..29878 (-) 1161 WP_102520942.1 radical SAM family heme chaperone HemW -
  VTAP4600_RS00180 (VTAP4600_A0030) - 29878..30477 (-) 600 WP_102520943.1 XTP/dITP diphosphatase -
  VTAP4600_RS00185 (VTAP4600_A0031) - 30531..30962 (-) 432 WP_102520944.1 DUF4426 domain-containing protein -
  VTAP4600_RS00190 (VTAP4600_A0032) yggU 30986..31276 (-) 291 WP_102520945.1 DUF167 family protein YggU -
  VTAP4600_RS00195 (VTAP4600_A0033) - 31276..31833 (-) 558 WP_102520946.1 YggT family protein -
  VTAP4600_RS00200 (VTAP4600_A0034) proC 31850..32668 (-) 819 WP_102520947.1 pyrroline-5-carboxylate reductase -
  VTAP4600_RS00205 (VTAP4600_A0035) - 32724..33434 (-) 711 WP_102520948.1 YggS family pyridoxal phosphate-dependent enzyme -
  VTAP4600_RS00210 (VTAP4600_A0036) pilT 33459..34496 (+) 1038 WP_102520949.1 type IV pilus twitching motility protein PilT Machinery gene
  VTAP4600_RS00215 (VTAP4600_A0037) pilU 34505..35611 (+) 1107 WP_102520950.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VTAP4600_RS00220 (VTAP4600_A0038) ruvX 35755..36177 (-) 423 WP_102520951.1 Holliday junction resolvase RuvX -
  VTAP4600_RS00225 (VTAP4600_A0039) - 36281..36847 (-) 567 WP_102520952.1 YqgE/AlgH family protein -
  VTAP4600_RS00230 (VTAP4600_A0040) gshB 36911..37858 (-) 948 WP_102520953.1 glutathione synthase -
  VTAP4600_RS00235 (VTAP4600_A0041) rsmE 37872..38603 (-) 732 WP_102520954.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  VTAP4600_RS00240 (VTAP4600_A0042) - 38685..39383 (-) 699 WP_102520955.1 endonuclease -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 37987.24 Da        Isoelectric Point: 6.1843

>NTDB_id=1148292 VTAP4600_RS00210 WP_102520949.1 33459..34496(+) (pilT) [Vibrio tapetis subsp. tapetis isolate Vibrio tapetis CECT4600]
MDITELLDFSVKHNASDLHLSAGVPPMVRVDGDVRKLGVPAFSHSDVHKMVFDIMNDAQRAEFEEKLETDFSFELPNVGR
FRVNAFNQSRGCSAVFRTIPTTVPTLDQISAPDIFTQISNYEKGLVLVTGPTGSGKSTTLAAMVDYVNRNHNKHILTIED
PIEFVHESQKCLINQREVHRDTHSFSAALRSALREDPDVILVGELRDKETISLALTAAETGHLVFGTLHTSSAAKTVDRI
IDVFPGADKDMVRSMLSESLRAVISQKLLKRLGGGRVACHEIMLATPAIRNLIREDKVAQMYSVIQTGAAMGMQTTEQHA
RQLIAQGAVDPQEVADKLESNNLGF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1148292 VTAP4600_RS00210 WP_102520949.1 33459..34496(+) (pilT) [Vibrio tapetis subsp. tapetis isolate Vibrio tapetis CECT4600]
ATGGATATCACTGAGTTATTGGATTTTAGTGTAAAGCATAATGCATCAGATCTACATCTTTCTGCGGGTGTTCCTCCAAT
GGTTCGCGTAGATGGCGATGTACGAAAGCTCGGAGTGCCTGCATTTAGTCATAGCGATGTGCATAAAATGGTGTTCGACA
TTATGAACGATGCGCAACGTGCCGAGTTTGAAGAAAAGCTAGAAACGGATTTTTCATTTGAACTGCCTAATGTGGGGCGA
TTCCGTGTTAATGCTTTTAATCAGTCCCGTGGTTGTTCTGCTGTGTTTCGAACCATCCCGACCACGGTGCCGACATTAGA
CCAAATATCCGCTCCTGATATTTTTACCCAGATCTCCAATTACGAAAAAGGCCTTGTTCTTGTGACGGGTCCTACCGGAT
CGGGTAAATCAACGACCCTTGCAGCCATGGTTGATTATGTTAATCGCAATCACAACAAACACATTTTAACGATTGAAGAC
CCGATTGAATTTGTTCATGAAAGCCAAAAATGCTTGATTAACCAACGTGAAGTTCACCGAGATACCCATAGCTTTAGTGC
GGCACTGCGTTCTGCTTTACGTGAAGATCCTGATGTTATTCTGGTGGGCGAGTTACGAGACAAAGAAACCATTAGCCTTG
CGTTAACGGCGGCAGAAACGGGTCACTTAGTGTTTGGTACGCTACACACCAGCAGCGCGGCAAAAACAGTAGACAGAATC
ATTGATGTTTTCCCAGGTGCAGATAAAGACATGGTGCGTTCTATGTTATCTGAGTCTTTGCGTGCGGTTATTTCTCAGAA
GTTACTTAAACGCCTGGGTGGCGGGCGCGTTGCCTGTCATGAAATCATGCTGGCTACTCCCGCAATTCGTAACCTTATTA
GAGAAGACAAAGTCGCGCAGATGTATTCTGTTATTCAAACGGGCGCGGCAATGGGGATGCAAACGACAGAGCAACACGCA
AGGCAATTGATAGCGCAAGGTGCGGTTGACCCACAAGAAGTGGCTGACAAGCTTGAATCGAATAACTTAGGCTTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2N8Z7X0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

86.136

98.261

0.846
  pilT Vibrio cholerae strain A1552

86.136

98.261

0.846
  pilT Acinetobacter baumannii D1279779

72.892

96.232

0.701
  pilT Acinetobacter baumannii strain A118

72.892

96.232

0.701
  pilT Acinetobacter nosocomialis M2

72.59

96.232

0.699
  pilT Acinetobacter baylyi ADP1

72.121

95.652

0.69
  pilT Pseudomonas stutzeri DSM 10701

70.03

97.681

0.684
  pilT Pseudomonas aeruginosa PAK

69.436

97.681

0.678
  pilT Legionella pneumophila strain Lp02

68.085

95.362

0.649
  pilT Legionella pneumophila strain ERS1305867

68.085

95.362

0.649
  pilT Neisseria meningitidis 8013

65.015

99.42

0.646
  pilT Neisseria gonorrhoeae MS11

65.465

96.522

0.632
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.023

100

0.522
  pilU Vibrio cholerae strain A1552

40.816

99.42

0.406
  pilU Pseudomonas stutzeri DSM 10701

40.233

99.42

0.4
  pilU Acinetobacter baylyi ADP1

39.375

92.754

0.365

Multiple sequence alignment