Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   VTAP4600_RS00215 Genome accession   NZ_LT960611
Coordinates   34505..35611 (+) Length   368 a.a.
NCBI ID   WP_102520950.1    Uniprot ID   A0A2N8Z807
Organism   Vibrio tapetis subsp. tapetis isolate Vibrio tapetis CECT4600     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 29505..40611
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VTAP4600_RS00180 (VTAP4600_A0030) - 29878..30477 (-) 600 WP_102520943.1 XTP/dITP diphosphatase -
  VTAP4600_RS00185 (VTAP4600_A0031) - 30531..30962 (-) 432 WP_102520944.1 DUF4426 domain-containing protein -
  VTAP4600_RS00190 (VTAP4600_A0032) yggU 30986..31276 (-) 291 WP_102520945.1 DUF167 family protein YggU -
  VTAP4600_RS00195 (VTAP4600_A0033) - 31276..31833 (-) 558 WP_102520946.1 YggT family protein -
  VTAP4600_RS00200 (VTAP4600_A0034) proC 31850..32668 (-) 819 WP_102520947.1 pyrroline-5-carboxylate reductase -
  VTAP4600_RS00205 (VTAP4600_A0035) - 32724..33434 (-) 711 WP_102520948.1 YggS family pyridoxal phosphate-dependent enzyme -
  VTAP4600_RS00210 (VTAP4600_A0036) pilT 33459..34496 (+) 1038 WP_102520949.1 type IV pilus twitching motility protein PilT Machinery gene
  VTAP4600_RS00215 (VTAP4600_A0037) pilU 34505..35611 (+) 1107 WP_102520950.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  VTAP4600_RS00220 (VTAP4600_A0038) ruvX 35755..36177 (-) 423 WP_102520951.1 Holliday junction resolvase RuvX -
  VTAP4600_RS00225 (VTAP4600_A0039) - 36281..36847 (-) 567 WP_102520952.1 YqgE/AlgH family protein -
  VTAP4600_RS00230 (VTAP4600_A0040) gshB 36911..37858 (-) 948 WP_102520953.1 glutathione synthase -
  VTAP4600_RS00235 (VTAP4600_A0041) rsmE 37872..38603 (-) 732 WP_102520954.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  VTAP4600_RS00240 (VTAP4600_A0042) - 38685..39383 (-) 699 WP_102520955.1 endonuclease -
  VTAP4600_RS00245 (VTAP4600_A0043) - 39456..39956 (-) 501 WP_102520956.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 40847.68 Da        Isoelectric Point: 6.0899

>NTDB_id=1148293 VTAP4600_RS00215 WP_102520950.1 34505..35611(+) (pilU) [Vibrio tapetis subsp. tapetis isolate Vibrio tapetis CECT4600]
MGLTEILAQMVSQQASDIYITVGSPCLLRIDGEMQPLGESLTEHQVHALLNSSMDEVRHGEYLRDKEANFAIVRDSGRFR
VSAFFQRELPGAVIRRIETQIPTFSELSLPDILQDMSIAKRGLVLVVGATGSGKSTTLAAMTGFRNQQRSGHILTVEDPI
EFVHQHGKCIVTQREVGLDTDSYEIALKNSLRQAPDMILIGEIRSRDTMQYAMTFAETGHLCMATLHANNANQAIERILH
LVPKEQKEQFLFDLSMNLRGVVAQQLIRDKNGKGRHGVFEILLNTPRVSELIRRGDLHELKSTMAKSNEAGMKTFDQSLY
DLVIADKITEEDAMHAADSANDLRLMLKTKRGDSFGGGALDGVQIDMG

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=1148293 VTAP4600_RS00215 WP_102520950.1 34505..35611(+) (pilU) [Vibrio tapetis subsp. tapetis isolate Vibrio tapetis CECT4600]
ATGGGATTAACTGAAATCTTGGCGCAAATGGTGTCTCAGCAAGCTTCGGATATTTACATCACCGTGGGTTCACCTTGTTT
ACTGCGTATTGATGGTGAGATGCAACCTTTAGGTGAGTCGTTAACCGAGCACCAAGTTCACGCGCTGTTAAATAGCAGCA
TGGATGAAGTCCGTCATGGTGAGTATTTGCGTGATAAAGAAGCAAACTTTGCCATTGTGAGGGACAGCGGCCGATTTCGT
GTGAGTGCTTTTTTTCAGCGTGAATTACCGGGTGCGGTGATCCGACGCATTGAAACCCAGATCCCAACTTTTTCTGAACT
AAGCTTGCCTGATATTTTACAGGACATGTCGATTGCCAAGCGAGGCTTGGTGTTGGTTGTTGGTGCTACAGGATCGGGTA
AATCGACGACACTTGCCGCCATGACAGGGTTTCGTAATCAGCAGCGCAGTGGTCATATTCTGACGGTAGAAGATCCCATA
GAGTTTGTGCATCAACATGGCAAGTGCATTGTCACTCAGCGTGAAGTTGGTCTTGATACTGACAGTTATGAAATTGCATT
GAAGAATTCATTAAGACAAGCGCCTGATATGATTTTGATCGGAGAGATCCGCTCAAGAGATACGATGCAATATGCCATGA
CCTTTGCCGAAACGGGGCACCTTTGTATGGCGACTTTGCATGCTAATAACGCGAATCAGGCGATCGAGCGGATCTTACAT
TTAGTACCCAAAGAGCAAAAAGAGCAGTTTTTGTTTGATCTATCGATGAACTTACGTGGTGTTGTCGCTCAACAGTTGAT
CCGAGATAAAAACGGAAAAGGCCGTCACGGTGTGTTTGAAATTCTGTTAAATACCCCAAGAGTCTCAGAGTTGATCCGTC
GTGGTGATCTTCATGAACTGAAGAGTACCATGGCCAAATCAAATGAAGCGGGTATGAAAACGTTCGATCAAAGCTTATAC
GATCTGGTGATTGCGGATAAGATCACCGAAGAAGATGCAATGCACGCTGCAGACTCTGCTAACGACCTACGCTTAATGCT
CAAAACAAAACGTGGTGATAGTTTTGGTGGTGGTGCATTGGATGGTGTGCAGATAGATATGGGGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2N8Z807

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

75.477

99.728

0.753
  pilU Pseudomonas stutzeri DSM 10701

55.367

96.196

0.533
  pilU Acinetobacter baylyi ADP1

51.567

95.38

0.492
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.027

91.576

0.394
  pilT Legionella pneumophila strain ERS1305867

42.773

92.12

0.394
  pilT Legionella pneumophila strain Lp02

42.773

92.12

0.394
  pilT Acinetobacter baylyi ADP1

42.229

92.663

0.391
  pilT Acinetobacter baumannii strain A118

42.478

92.12

0.391
  pilT Acinetobacter nosocomialis M2

42.478

92.12

0.391
  pilT Acinetobacter baumannii D1279779

42.478

92.12

0.391
  pilT Pseudomonas aeruginosa PAK

41.593

92.12

0.383
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.462

88.315

0.375
  pilT Vibrio cholerae strain A1552

42.462

88.315

0.375
  pilT Pseudomonas stutzeri DSM 10701

39.823

92.12

0.367

Multiple sequence alignment