Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   KSMBR1_RS14335 Genome accession   NZ_LT934425
Coordinates   3073559..3074665 (+) Length   368 a.a.
NCBI ID   WP_099325934.1    Uniprot ID   A0A2C9CI02
Organism   Candidatus Kuenenia stuttgartiensis isolate kuenenia_mbr1_ru-nijmegen     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3068559..3079665
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KSMBR1_RS14320 (KSMBR1_2858) ligA 3068975..3070999 (+) 2025 WP_099325931.1 NAD-dependent DNA ligase LigA -
  KSMBR1_RS14325 (KSMBR1_2859) - 3071128..3072489 (+) 1362 WP_099325932.1 TldD/PmbA family protein -
  KSMBR1_RS14330 (KSMBR1_2860) pilT 3072490..3073539 (+) 1050 WP_099325933.1 type IV pilus twitching motility protein PilT Machinery gene
  KSMBR1_RS14335 (KSMBR1_2861) pilU 3073559..3074665 (+) 1107 WP_099325934.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KSMBR1_RS14340 (KSMBR1_2862) - 3074763..3075008 (-) 246 WP_099325935.1 HicB family protein -
  KSMBR1_RS14345 (KSMBR1_2863) - 3075022..3075213 (-) 192 WP_099325936.1 type II toxin-antitoxin system HicA family toxin -
  KSMBR1_RS14350 (KSMBR1_2864) - 3075291..3076166 (-) 876 WP_099325937.1 exonuclease domain-containing protein -
  KSMBR1_RS14355 (KSMBR1_2865) - 3076185..3076556 (-) 372 WP_099325938.1 hypothetical protein -
  KSMBR1_RS14360 (KSMBR1_2866) - 3076836..3077234 (-) 399 WP_099325939.1 hypothetical protein -
  KSMBR1_RS14365 (KSMBR1_2867) - 3077527..3077739 (-) 213 WP_099325940.1 type II toxin-antitoxin system HicB family antitoxin -
  KSMBR1_RS14370 (KSMBR1_2868) - 3077739..3077993 (-) 255 WP_099325941.1 toxin HicA -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41598.96 Da        Isoelectric Point: 6.5990

>NTDB_id=1148291 KSMBR1_RS14335 WP_099325934.1 3073559..3074665(+) (pilU) [Candidatus Kuenenia stuttgartiensis isolate kuenenia_mbr1_ru-nijmegen]
MEIKDLLQEMVRLDASDIYITVGLPPVYRKEGNNIPAGQEMVTGDDTRTLAESIMSEKQRADFYEQMEMNLALYYPELGR
FRVNIFFQQRNVGLVIRQIKINIQSIDDLRLPQVFKDIAMTKRGLVLVVGATGSGKSTTLAAMIDHRNENNSGHIITVED
PIEFVHRHKKSVITQREIGLDSLSFHNALKNTLRQAPDVILVGEIRDTETMESAITFAETGHLCLGTLHANNANQAIERI
INFFPPERHEQIYMLLSLNLRAIISQRLIPAKDGKRSGAFEVLLDTPRIKDLILKKEIGLLKETMAKGNQEGMMTLDQSL
FHLLKKGEITYENAIAYADSANDLRLRIKVEGIEGEKEEGQAARFKLK

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=1148291 KSMBR1_RS14335 WP_099325934.1 3073559..3074665(+) (pilU) [Candidatus Kuenenia stuttgartiensis isolate kuenenia_mbr1_ru-nijmegen]
ATGGAAATAAAAGATTTATTACAAGAAATGGTGCGTTTGGATGCTTCTGATATTTACATTACCGTTGGGCTGCCCCCTGT
GTACAGGAAGGAAGGGAACAACATTCCCGCCGGTCAGGAAATGGTAACGGGCGATGACACCCGCACCCTTGCCGAATCAA
TAATGAGCGAAAAACAAAGGGCAGATTTTTACGAACAGATGGAAATGAATTTAGCCCTGTATTATCCGGAACTTGGACGT
TTCAGGGTAAACATTTTTTTTCAGCAAAGGAATGTCGGCCTTGTTATCCGGCAGATAAAGATAAATATTCAGTCTATTGA
CGATTTACGGCTACCCCAGGTGTTCAAAGACATTGCCATGACAAAACGGGGGCTTGTGCTGGTAGTTGGCGCAACCGGTT
CAGGGAAATCAACCACACTGGCGGCGATGATTGATCACAGGAATGAGAATAATTCAGGCCACATAATTACGGTAGAAGAC
CCCATTGAGTTTGTGCACCGCCATAAAAAGTCTGTTATCACACAAAGAGAAATCGGACTGGACAGCTTGTCGTTTCACAA
TGCACTAAAAAATACCTTGCGCCAGGCGCCGGACGTCATCCTGGTGGGAGAAATCCGCGATACCGAAACAATGGAGTCTG
CCATTACCTTTGCGGAAACGGGCCATCTTTGTTTGGGAACACTTCATGCAAACAACGCAAACCAGGCGATTGAAAGGATT
ATAAACTTCTTTCCTCCGGAACGGCATGAACAAATATACATGCTCCTCTCGCTGAATCTACGGGCAATAATCTCGCAAAG
ATTAATTCCAGCAAAAGATGGAAAGCGGTCAGGCGCCTTTGAAGTATTGCTGGACACCCCAAGGATAAAGGATCTTATCT
TAAAAAAGGAAATAGGGTTATTGAAGGAAACAATGGCAAAAGGGAATCAGGAAGGGATGATGACCCTGGACCAGTCCTTA
TTTCATCTTTTAAAAAAGGGGGAAATAACTTACGAAAACGCAATTGCCTACGCCGATAGCGCGAATGATCTGCGTCTGCG
GATAAAGGTGGAAGGGATTGAAGGTGAGAAAGAAGAAGGGCAGGCTGCGCGTTTTAAATTAAAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(93-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2C9CI02

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.986

95.38

0.524
  pilU Vibrio cholerae strain A1552

53.125

95.652

0.508
  pilU Acinetobacter baylyi ADP1

52.841

95.652

0.505
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.903

92.12

0.432
  pilT Acinetobacter baylyi ADP1

45.058

93.478

0.421
  pilT Acinetobacter baumannii D1279779

44.97

91.848

0.413
  pilT Acinetobacter baumannii strain A118

44.97

91.848

0.413
  pilT Acinetobacter nosocomialis M2

44.97

91.848

0.413
  pilT Legionella pneumophila strain Lp02

43.787

91.848

0.402
  pilT Legionella pneumophila strain ERS1305867

43.787

91.848

0.402
  pilT Pseudomonas stutzeri DSM 10701

43.491

91.848

0.399
  pilT Pseudomonas aeruginosa PAK

43.195

91.848

0.397
  pilT Neisseria meningitidis 8013

42.773

92.12

0.394
  pilT Neisseria gonorrhoeae MS11

42.773

92.12

0.394
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.553

89.402

0.38
  pilT Vibrio cholerae strain A1552

42.553

89.402

0.38

Multiple sequence alignment