Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KSMBR1_RS14330 Genome accession   NZ_LT934425
Coordinates   3072490..3073539 (+) Length   349 a.a.
NCBI ID   WP_099325933.1    Uniprot ID   -
Organism   Candidatus Kuenenia stuttgartiensis isolate kuenenia_mbr1_ru-nijmegen     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3067490..3078539
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KSMBR1_RS14315 (KSMBR1_2857) - 3067562..3068518 (-) 957 WP_099325930.1 hypothetical protein -
  KSMBR1_RS14320 (KSMBR1_2858) ligA 3068975..3070999 (+) 2025 WP_099325931.1 NAD-dependent DNA ligase LigA -
  KSMBR1_RS14325 (KSMBR1_2859) - 3071128..3072489 (+) 1362 WP_099325932.1 TldD/PmbA family protein -
  KSMBR1_RS14330 (KSMBR1_2860) pilT 3072490..3073539 (+) 1050 WP_099325933.1 type IV pilus twitching motility protein PilT Machinery gene
  KSMBR1_RS14335 (KSMBR1_2861) pilU 3073559..3074665 (+) 1107 WP_099325934.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KSMBR1_RS14340 (KSMBR1_2862) - 3074763..3075008 (-) 246 WP_099325935.1 HicB family protein -
  KSMBR1_RS14345 (KSMBR1_2863) - 3075022..3075213 (-) 192 WP_099325936.1 type II toxin-antitoxin system HicA family toxin -
  KSMBR1_RS14350 (KSMBR1_2864) - 3075291..3076166 (-) 876 WP_099325937.1 exonuclease domain-containing protein -
  KSMBR1_RS14355 (KSMBR1_2865) - 3076185..3076556 (-) 372 WP_099325938.1 hypothetical protein -
  KSMBR1_RS14360 (KSMBR1_2866) - 3076836..3077234 (-) 399 WP_099325939.1 hypothetical protein -
  KSMBR1_RS14365 (KSMBR1_2867) - 3077527..3077739 (-) 213 WP_099325940.1 type II toxin-antitoxin system HicB family antitoxin -
  KSMBR1_RS14370 (KSMBR1_2868) - 3077739..3077993 (-) 255 WP_099325941.1 toxin HicA -

Sequence

Protein


Download         Length: 349 a.a.        Molecular weight: 38809.91 Da        Isoelectric Point: 6.6654

>NTDB_id=1148290 KSMBR1_RS14330 WP_099325933.1 3072490..3073539(+) (pilT) [Candidatus Kuenenia stuttgartiensis isolate kuenenia_mbr1_ru-nijmegen]
MDIMELLAFATKESASDVHISSGEPPMIRIHGDMRKIDMPPLMREDVHTLLYNILNDQQRKVYEEHYELDFAIAITGVGR
FRVNAFLQNRGESIVFRTIPEKIPLLEQLNMPPIVAELTKKEKGLILVTGPTGSGKSTTLAAMLDLINREYKCHILTVED
PIEFVHKSKNSLVNQRELGSNTHSFSNALRSALREDPDVILVGEMRDLETISLALTAAETGHLVLATLHTSSAPKTVDRI
IDVFPAEQQQQIRTMLSESLQAVIAQQLIKRKEGVGRIAALEIMIGTPAVRNLIRENKIAQIPSALQTGRQHGMQTMDQA
LLDLCRNGLVSKESIRNFVSAPSVLDGIK

Nucleotide


Download         Length: 1050 bp        

>NTDB_id=1148290 KSMBR1_RS14330 WP_099325933.1 3072490..3073539(+) (pilT) [Candidatus Kuenenia stuttgartiensis isolate kuenenia_mbr1_ru-nijmegen]
ATGGATATAATGGAACTCCTCGCTTTTGCAACCAAAGAAAGCGCGTCGGATGTGCATATAAGTTCCGGTGAACCGCCCAT
GATCCGTATACATGGTGATATGAGAAAAATTGACATGCCGCCATTGATGAGGGAGGATGTGCATACGCTTTTGTATAATA
TTCTTAATGACCAGCAACGCAAGGTATATGAGGAACATTATGAATTGGATTTTGCCATTGCAATAACGGGCGTTGGCCGT
TTCAGGGTGAACGCCTTTCTGCAGAACAGGGGCGAGTCTATTGTGTTCAGAACCATTCCAGAAAAAATCCCGCTTCTGGA
ACAACTTAATATGCCCCCTATTGTGGCAGAATTAACGAAAAAGGAGAAGGGGCTCATCCTCGTAACAGGACCTACCGGCA
GCGGAAAATCAACCACGCTGGCGGCTATGCTAGACCTCATTAACCGGGAATATAAATGTCATATCCTTACCGTTGAAGAC
CCCATAGAATTTGTCCATAAGTCCAAAAACAGCCTCGTTAATCAAAGGGAATTGGGGTCAAATACCCATAGTTTCTCTAA
CGCCCTGAGATCGGCGCTTCGCGAAGACCCTGATGTTATTCTGGTGGGAGAAATGAGAGACCTTGAGACCATATCGCTGG
CGCTCACCGCCGCAGAAACCGGTCATCTCGTACTTGCCACGTTGCATACTTCCAGTGCGCCGAAAACGGTTGACAGGATA
ATTGATGTATTTCCCGCGGAACAACAACAGCAAATCCGCACCATGCTGTCCGAATCGCTTCAGGCGGTAATTGCCCAACA
ACTGATCAAGAGAAAGGAAGGGGTTGGGCGTATCGCAGCGTTAGAAATTATGATCGGCACCCCTGCCGTAAGAAATTTGA
TCAGGGAGAATAAGATTGCCCAAATCCCTTCGGCATTGCAAACCGGACGCCAGCACGGCATGCAGACAATGGACCAGGCG
CTGTTGGATTTGTGCAGAAACGGCCTTGTTTCCAAAGAATCAATCAGGAATTTTGTAAGCGCCCCCTCTGTCCTCGACGG
CATAAAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

63.158

97.994

0.619
  pilT Acinetobacter nosocomialis M2

62.573

97.994

0.613
  pilT Acinetobacter baumannii D1279779

62.573

97.994

0.613
  pilT Acinetobacter baumannii strain A118

62.573

97.994

0.613
  pilT Pseudomonas stutzeri DSM 10701

62.573

97.994

0.613
  pilT Legionella pneumophila strain ERS1305867

63.174

95.702

0.605
  pilT Legionella pneumophila strain Lp02

63.174

95.702

0.605
  pilT Vibrio cholerae strain A1552

62.018

96.562

0.599
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

62.018

96.562

0.599
  pilT Acinetobacter baylyi ADP1

60.819

97.994

0.596
  pilT Neisseria meningitidis 8013

61.31

96.275

0.59
  pilT Neisseria gonorrhoeae MS11

61.012

96.275

0.587
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.652

95.415

0.493
  pilU Acinetobacter baylyi ADP1

41.618

99.14

0.413
  pilU Pseudomonas stutzeri DSM 10701

41.441

95.415

0.395
  pilU Vibrio cholerae strain A1552

40.541

95.415

0.387

Multiple sequence alignment